We narrowed to 23,637 results for: promoter

1. pSt1374m-EF1alpha-N-NLS-DNMT3L-L-DNMT3Amut-L-dcas9-NLS

   Plasmid

   #112215

   Purpose

   Targeted DNA methylation

   Depositor

   Xingxu Huang

   Article

   Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

   Insert

   DNMT3L-L-DNMT3Amut-L-dcas9 (DNMT3A, DNMT3L Human)
   

   Use

   CRISPR

   Tags

   mCherry

   Mutation

   D10A,H840A,N863A,C710S

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-CRISPR-V2-gYap-2

   Plasmid

   #166482

   Purpose

   Expresses spCas9 and a gRNA targeting mouse Yap

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   gRNA for mouse Yap (Yap1 Mouse)
   

   Use

   Lentiviral

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV-Neo-GFP/Flag-Gata5

   Plasmid

   #105508

   Purpose

   MSCV-driven retroviral Gata5 expression (Flag-tagged)

   Depositor

   Christopher Vakoc

   Article

   Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

   Insert

   Gata5 (Gata5 Mouse)
   

   Use

   Retroviral

   Tags

   Flag

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. mEGFP-N1 YAPS127A- L308E

   Plasmid

   #166462

   Purpose

   Expresses fusion of mEGFP and YAPS127A- L308E

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   mEGFP, YAPS127A- L308E (YAP1 Human)
   

   Expression

   Mammalian

   Mutation

   YAPS127A- L308E

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. mEGFP-N1 YAPS127A-deltasIDR

   Plasmid

   #166460

   Purpose

   Expresses fusion of mEGFP and YAPdeltasIDR (delta360-449)

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   mEGFP, YAPS127A-deltasIDR (YAP1 Human)
   

   Expression

   Mammalian

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. mEGFP-N1 YAPS127A-deltasTAD

   Plasmid

   #166461

   Purpose

   Expresses fusion of mEGFP and YAPdeltasTAD (delta450-504)

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   mEGFP, YAPS127A-deltasTAD (YAP1 Human)
   

   Expression

   Mammalian

   Available Since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-Cry2-mCherry-coiled coil

   Plasmid

   #166448

   Purpose

   Expresses fusion of CRY2PHR with mCherry and YAP Coiled-coil domain

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   Cry2, mCherry, coiled coil(298-359) (CRY2 Human)
   

   Use

   Lentiviral

   Available Since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cavin4a in pBluescript(SK+)

   Plasmid

   #126567

   Purpose

   For generating RNA probes for in situ hybridisation. Parton lab clone ISB

   Depositor

   Rob Parton

   Article

   Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

   Insert

   Cavin4a (cavin4a Zebrafish)
   

   Use

   Gateway entry clone

   Available Since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. mEGFP-N1-NF2

   Plasmid

   #166473

   Purpose

   Expresses fusion of mEGFP and NF2

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   mEGFP, NF2 (NF2 Human)
   

   Expression

   Mammalian

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. VN-PTEN

    Plasmid

    #89331

    Purpose

    expresses hPTEN fused to the N-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Marciniak et al J Exp Med. 2017 Aug 7;214(8):2257-2269.

    Insert

    PTEN1 (PTEN Human)
    

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-EF1α1.1-FLEX-rc [ChrimsonR-GFP]

    Plasmid

    #108273

    Purpose

    AAV-mediated expression of ChrimsonR-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    EF1α promoter (1.1kb short version)

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-FLAG_FBXL7 (R310H)

    Plasmid

    #171849

    Purpose

    pcDNA3-FLAG_FBXL7 (R310H)

    Depositor

    Michele Pagano

    Article

    Moro et al Nat Cell Biol. 2020 Sep;22(9):1130-1142.

    Insert

    F-box and leucine rich repeat protein 7 (FBXL7 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed Arginine 310 to Histidine

    Available Since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. mEGFP-N1 YAPS127A- L325E

    Plasmid

    #166464

    Purpose

    Expresses fusion of mEGFP and YAPS127A- L325E

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mEGFP, YAPS127A- L325E (YAP1 Human)
    

    Expression

    Mammalian

    Mutation

    YAPS127A- L325E

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. MIGR1-Cdc42-2

    Plasmid

    #179518

    Purpose

    Retroviral expression of Cdc42 splice variant 2

    Depositor

    Bo Porse

    Article

    Rehn et al Cell Rep. 2022 May 10;39(6):110793. doi:

    Insert

    Cdc42 splice variant 2 cDNA (CDC42 Human)
    

    Use

    Retroviral

    Tags

    IRES-EGFP

    Promoter

    LTR

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid

    #196074

    Purpose

    (Empty Backbone) Constitutive CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Inserts

    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    

    Expression

    Mammalian

    Mutation

    Catalytically dead mutant of the Cas9 endonucleas…

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL3-noSV40-humanEC1.45_min1-2_1XCoordinatorMutant

    Plasmid

    #173963

    Purpose

    Firefly luciferase enhancer reporter plasmid.

    Depositor

    Joanna Wysocka

    Article

    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

    Insert

    Minimal enhancer regions 1 and 2 (min1 and min2) from SOX9 enhancer cluster EC1.45 with 1 mutated Coordinator motif
    

    Use

    Luciferase

    Mutation

    1 mutated Coordinator motif

    Promoter

    SV40 promoter

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR221-SARS-CoV-2 S1 RBD

    Plasmid

    #167013

    Purpose

    Gateway-compatible Entry vector encoding spike (S1) RBD domain from SARS-CoV-2

    Depositor

    Spyros Petrakis

    Article

    Gkekas et al J Chem Inf Model. 2024 Nov 13. doi: 10.1

    Insert

    S (S SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Expression

    Mammalian

    Mutation

    No stop codon

    Available Since

    April 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pCMV-T7-ABE8e-SpCas9-HF1-P2A-EGFP (LM422)

    Plasmid

    #197505

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HF1(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-HF1-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-HF1(D10A/N497…

    Promoter

    CMV and T7

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. mEGFP-N1 YAPS127A- L318E

    Plasmid

    #166465

    Purpose

    Expresses fusion of mEGFP and YAPS127A- L318E

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    mEGFP, YAPS127A- L318E (YAP1 Human)
    

    Expression

    Mammalian

    Mutation

    YAPS127A- L318E

    Available Since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCEE

    Plasmid

    #159746

    Purpose

    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.

    Depositor

    Wolfgang Lukowitz

    Article

    Angulo et al bioRxiv

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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