We narrowed to 18,937 results for: Tec

1. pLEX307-HCoV-NL63-3CL

   Plasmid

   #160286

   Purpose

   Allows for constituitive mammalian expression of the HCoV-NL63 3CL protease via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIRES-hrGFP II-mTET1

   Plasmid

   #83569

   Purpose

   Expresses TET1 with a mutated catalytic domain in mammalian cells

   Depositor

   Jean-Pierre Issa

   Article

   Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

   Insert

   Tet Methylcytosine Dioxygenase 1 (TET1 Human)
   

   Tags

   3X FLAG tag and hr GFP II

   Expression

   Mammalian

   Mutation

   catalytic domain mutant H1672D, D1674A

   Promoter

   CMV

   Available Since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX307-Bat-CoV-HKU9-3CL

   Plasmid

   #160284

   Purpose

   Allows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV_UdgX-EE-UdgX-NG-nCas9-UdgX

   Plasmid

   #163572

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-EE-UdgX-NG-nCas9-UdgX
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACgp67B-Her2mP

   Plasmid

   #10793

   Depositor

   Judy Lieberman

   Article

   Song et al Nat Biotechnol. 2005 Jun . 23(6):709-17.

   Insert

   ErbB2 ScFv (ERBB2 Human)
   

   Tags

   6His and Truncated Protamine

   Expression

   Insect

   Available Since

   Aug. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. RPS6KA1 gRNA (BRDN0001148103)

   Plasmid

   #75498

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human RPS6KA1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   RPS6KA1 (RPS6KA1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. CLXSN 5myc-CDC6-mut

   Plasmid

   #109333

   Purpose

   express tagged Cdc6 mutant

   Depositor

   Jean Cook

   Article

   Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

   Insert

   CDC6 (CDC6 Human)
   

   Use

   Retroviral

   Tags

   5x Myc

   Expression

   Mammalian

   Mutation

   R56A, L59A, K81A, E82A, N83A

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. TBK1 gRNA (BRDN0001148392)

   Plasmid

   #76362

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TBK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TBK1 (TBK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. RPS6KA2 gRNA (BRDN0001148851)

   Plasmid

   #75734

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human RPS6KA2

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   RPS6KA2 (RPS6KA2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCold I-Hero11

    Plasmid

    #187928

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero11

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero11 (C19orf53 Human)
    

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Met1 diUb (1-152)

    Plasmid

    #110766

    Purpose

    Bacterial expression of Met1 diUb

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    UBB (UBB Human)
    

    Tags

    N-His6-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. TBK1 gRNA (BRDN0001145663)

    Plasmid

    #76361

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TBK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TBK1 (TBK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7-G-LbCas12a crRNA-BsaI cassette (MSP3495)

    Plasmid

    #114068

    Purpose

    T7 promoter crRNA entry vector used for in vitro transcription of LbCas12a guides

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for LbCas12a crRNAs
    

    Use

    In vitro transcription

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pINTO-NFH::mEzh2

    Plasmid

    #65925

    Purpose

    Expression of mouse Ezh2 fused to Flag-HA tag (N-terminus)

    Depositor

    Roberto Bonasio

    Article

    Beck et al J Proteome Res. 2014 Dec 5;13(12):6135-4

    Insert

    Enhancer of zeste homolog 2 (Ezh2 Mouse)
    

    Tags

    FLAG-HA

    Expression

    Mammalian

    Promoter

    CMV/TO (T-REx) and CMV/TetO2 (T-REx)

    Available Since

    July 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. RPS6KA2 gRNA (BRDN0001147334)

    Plasmid

    #75732

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RPS6KA2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RPS6KA2 (RPS6KA2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLEX307-SARS-CoV-3CL

    Plasmid

    #160280

    Purpose

    Allows for constituitive mammalian expression of the SARS-CoV 3CL protease via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Agarabi CHO Heavy Chain in pJ201

    Plasmid

    #111567

    Purpose

    Cloning vector containing CDS for the modified human heavy chain of the model chimeric mouse-human IgG1 expressed by the Agarabi CHO cell line

    Depositor

    Cyrus Agarabi

    Article

    Velugula-Yellela et al Biotechnol Prog. 2018 Jan;34(1):262-270.

    Insert

    Human heavy chain
    

    Use

    Synthetic Biology; Cloning vector

    Promoter

    None

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET19-MTdTc302

    Plasmid

    #207458

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    MTdTc302 (Dntt Mouse)
    

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Mutation

    Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser

    Available Since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHRIG-PRAS40

    Plasmid

    #53599

    Purpose

    lentiviral expression of constitutively active PRAS40 and EGFP

    Depositor

    Heng Zhao

    Article

    Xiong et al Neurobiol Dis. 2014 Jun;66:43-52. doi: 1

    Insert

    PRAS40 (AKT1S1 Human)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP and myc

    Expression

    Mammalian

    Mutation

    constitutively active (myristoylated version)

    Promoter

    CMV

    Available Since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

20. PKN1 gRNA (BRDN0001162558)

    Plasmid

    #76278

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PKN1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PKN1 (PKN1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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