174,739 results

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Teto BRN3A

Plasmid

#62221

Purpose

human BRN3A cDNA

Depositor

Kristin Baldwin

Article

Blanchard et al Nat Neurosci. 2015 Jan;18(1):25-35. doi:

Insert

BRN3A (POU4F1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

deleted a.a. 142-181

Available Since

April 14, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRNA7-mGFP

Plasmid

#62629

Purpose

mammalian expression of human Alpha7 (CHRNA7) with mEGFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

hAlpha7 (CHRNA7 Human)

Tags

mEGFP inserted in M3-M4 loop

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2015

Availability

Academic Institutions and Nonprofits only

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PX852

Plasmid

#62884

Purpose

C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa536-1368)

Use

CRISPR

Tags

FKBP12, NLS SV40, and NLS nucleoplasmin

Expression

Mammalian

Promoter

CBh

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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PX851

Plasmid

#62883

Purpose

N-term SpCas9 piece of inducible split-4 (Cas9(N)-FRB-NES split-4).

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa 2-535)

Use

CRISPR

Tags

FRB and NES PTK2

Expression

Mammalian

Promoter

CBh

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2-epNTR

Plasmid

#62213

Purpose

epNTR (zebrafish optimized nitroreductase, T41L, N71S, F124W) in pCS2

Depositor

Harold Burgess

Article

Tabor et al J Neurophysiol. 2014 Aug 15;112(4):834-4

Insert

epNTR

Use

Mrna synthesis

Available Since

Feb. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB

Plasmid

#52889

Purpose

"CoinFLP-Gal4" - Expresses Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express Gal4, or FRT3-FRT3 pair to prevent Gal4 expression

Depositor

Iswar Hariharan

Article

Bosch et al Development. 2015 Feb 1;142(3):597-606.

Insert

Gal4 (GAL4 Budding Yeast)

Expression

Insect

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pHR SFFVp BFP-Erk2

Plasmid

#50848

Purpose

Fluorescent reporter for Erk nuclear translocation

Depositor

Wendell Lim

Article

Toettcher et al Cell. 2013 Dec 5;155(6):1422-34. doi: 10

Insert

Erk2 (Mapk1 Mouse)

Use

Lentiviral

Tags

TagBFP

Available Since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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Dendra2-Actin-C-18

Plasmid

#57701

Purpose

Localization: Actin, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Actin (ACTB Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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Nanog-2A-GFP

Plasmid

#59994

Purpose

Inserts GFP immediately downstream of the Nanog coding region

Depositor

Rudolf Jaenisch

Article

Faddah et al Cell Stem Cell. 2013 Jul 3;13(1):23-9. d

Insert

Nanog (Nanog Mouse)

Use

Mouse Targeting

Tags

2A-GFP

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry-Alpha-5-Integrin-12

Plasmid

#54970

Purpose

Localization: Focal Adhesions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Alpha-5 Integrin (ITGA5 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pJG/ALPHA MHC

Plasmid

#55594

Purpose

transgene expression driven by ALPHA MHC promoter

Depositor

Jeffrey Robbins

Article

Gulick et al J Biol Chem. 1991 May 15;266(14):9180-5.

Type

Empty backbone

Expression

Mammalian

Promoter

alpha MHC

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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mEmerald-Integrin-Beta3-N-18

Plasmid

#54130

Purpose

Localization: Integrin/Focal Adhesions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Integrin-Beta3

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mVenus-C1

Plasmid

#54651

Purpose

Localization: C1 Cloning Vector, Excitation: 515, Emission: 528

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

Type

Empty backbone

Tags

mVenus

Expression

Mammalian

Available Since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3.1 3xFlag IFIT2

Plasmid

#53555

Purpose

mammalian expression of IFIT2

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT2 (IFIT2 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pLXSN18E6E7

Plasmid

#53459

Purpose

The plasmid, pLXSN18E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV18 E6 and E7 genes.

Depositor

Denise Galloway

Article

Halbert et al J Virol. 1991 Jan;65(1):473-8.

Insert

HPV18E6E7

Use

Retroviral

Available Since

May 27, 2014

Availability

Academic Institutions and Nonprofits only

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pIRES-HA-14-3-3 ϵ/myc-14-3-3ζ

Plasmid

#48795

Purpose

expresses human HA-14-3-3 ϵ (epsilon) and myc-14-3-3ζ (zeta) in mammalian cells

Depositor

Huda Zoghbi

Article

Chen et al Cell. 2003 May 16;113(4):457-68.

Inserts

YWHAE (YWHAE Human)
YWHAZ (YWHAZ Human)

Tags

HA and myc

Expression

Mammalian

Promoter

CMV and CMV (IRES)

Available Since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

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PH-Akt(R25C)-GFP

Plasmid

#51466

Purpose

Non-PIP3 binding control for PH-Btk-GFP

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

AKT (aa 1–164)

Tags

EGFP

Expression

Mammalian

Mutation

R25C

Promoter

CMV

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only
pBEVY-GL

Plasmid

#51225

Purpose

bi-directional expression vector for yeast, galactose inducible, LEU2 selection

Depositor

Charles Miller

Article

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Expression

Yeast

Promoter

GAL1,10

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pET-53-RtcB

Plasmid

#51282

Purpose

E. coli expression vector for E. coli RtcB RNA ligase

Depositor

Jay Hesselberth

Article

Peach et al Nucleic Acids Res. 2015 May 22. pii: gkv

Insert

E. coli RtcB

Tags

6xHis

Expression

Bacterial

Promoter

T7 RNA Polymerase

Available Since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2+ Gal4-GBP2-IRES-GBP7-p65

Plasmid

#50020

Purpose

Multi-purpose expression of a GFP-dependent transcription factor

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

Gal4DBD-GBP2-IRES-NLS-GBP7-p65AD

Use

Multi-purpose expression

Promoter

CMV-immediate early promoter and SP6 promoter

Available Since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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