We narrowed to 11,803 results for: nsf

1. (565) pAAV Alb-AAT KRAB-SadCas9 U6-gSTOP

   Plasmid

   #163030

   Purpose

   Expression of CRISPRi with gSTOP gRNA

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   AAV

   Tags

   KRAB, SV40 pA, and myc NLS

   Expression

   Mammalian

   Promoter

   Alb-AAT

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV Syn CaMPARI2-F391W_L398V-human-a-Tubulin (high affinity TubuTag)

   Plasmid

   #164193

   Purpose

   Freeze-frame imaging of dendritic calcium signals High calcium affinity (CaMPARI2_F391W_L398V moiety Kd=174nM)

   Depositor

   Thomas Oertner

   Article

   Perez-Alvarez et al Front Mol Neurosci. 2021 Mar 4;14:635820

   Inserts

   CamPARI2
   Human α Tubulin
   

   Use

   AAV

   Tags

   6 His tag and NES

   Expression

   Mammalian

   Mutation

   F391W-L398V

   Promoter

   Syn

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Hbb

   Plasmid

   #99692

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Hbb, vector allows for strong activation of mouse Hbb, can be packaged and delivered as AAV

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9 and gRNA targeting Hbb (Hbb Synthetic)
   

   Use

   AAV

   Tags

   VPR mini

   Mutation

   dead Cas9

   Available Since

   Jan. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hsyn-GRAB_rDA-mut

   Plasmid

   #140558

   Purpose

   Expresses the red fluorescent DA-insensitive control sensor GRAB_rDA-mut in neurons

   Depositor

   Yulong Li

   Article

   Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

   Has Service

   AAV9

   Insert

   Red fluorescent DA-insensitive sensor GRAB_rDA-mut (control sensor)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CBh-IgKL-mNG

   Plasmid

   #183467

   Purpose

   To produce AAV with the CBh promoter to express IgkL-mNeonGreen (IgKL is a secretory signal peptide)

   Depositor

   Hajime Hirase

   Article

   Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

   Insert

   IgKL-mNeonGreen
   

   Use

   AAV

   Tags

   IgKL secretory signal

   Available Since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-DIO-ChroME2f-ST-P2A-H2B-mRuby3

   Plasmid

   #171150

   Purpose

   Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

   Depositor

   Hillel Adesnik

   Article

   Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

   Insert

   ChroME2f-P2A-H2B-mRuby3
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV CMV-DIO-(mCherry-U6)-shRNA(scrambled)

   Plasmid

   #214733

   Purpose

   Expresses mCherry protein in infected and cre positive cells

   Depositor

   Félix Leroy

   Article

   de Leon Reyes et al Cell. 2023 Sep 14;186(19):4152-4171.e31.

   Insert

   anti-Crh shRNA (scrambled) / mCherry
   

   Use

   AAV

   Available Since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-DIO-Bmal1

   Plasmid

   #239045

   Purpose

   Overexpression of Bmal1

   Depositor

   Qingchun Tong

   Article

   Kim et al Nat Commun. 2020 Jul 30;11(1):3794. doi:

   Insert

   mArntl (Bmal1 Mouse)
   

   Use

   AAV

   Promoter

   Bmal1

   Available Since

   May 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-actin prom-dsRed-scramble shRNA

   Plasmid

   #67879

   Purpose

   AAV vector to express dsRed sh RNA (scramble)

   Depositor

   William Wisden

   Article

   Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

   Insert

   actin prom-dsRed-scramble shRNA
   

   Use

   AAV and RNAi

   Expression

   Mammalian

   Promoter

   chicken beta actin

   Available Since

   Aug. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

10. PP_071_pAAV_CAG_DIO-LR-Voltron2-P2A-LR-CheRiff-TS-eYFP-ER2

    Plasmid

    #203229

    Purpose

    Cre-dependent version of PP_070. Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

    Depositor

    Adam Cohen

    Insert

    Cre-dependent, membrane-localized Optopatch
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSYN-DRD2-V2tail-TevN-BLITz1-TetR-VP16-bGHpA

    Plasmid

    #89874

    Purpose

    expresses DRD2-iTango component in mammalian cells for AAV production

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    DRD2-V2tail-TevN-BLITz1-TetR-VP16
    

    Use

    AAV

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    short isoform of DRD2 (deletion of 242-270)

    Promoter

    Human synapsin

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-GRAB_NEmut

    Plasmid

    #123310

    Purpose

    Expresses the genetically-encoded fluorescent norepinephrine(NE) control sensor GRAB_NEmut in neurons

    Depositor

    Yulong Li

    Article

    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d

    Has Service

    AAV Retrograde and AAV9

    Insert

    GPCR activation based NE control sensor GRAB_NEmut
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV CMV-DIO-(mCherry-U6)-shRNA(anti-Crh)

    Plasmid

    #214732

    Purpose

    Expresses shRNA against CRH, marked with mCherry, in infected and cre positive cells

    Depositor

    Félix Leroy

    Article

    de Leon Reyes et al Cell. 2023 Sep 14;186(19):4152-4171.e31.

    Insert

    anti-Crh shRNA / mCherry
    

    Use

    AAV

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-gfaABC1D-HA-eLACCO2.1-NGR

    Plasmid

    #208098

    Purpose

    Biosensor for extracellular L-lactate

    Depositor

    Robert Campbell, Marie-Eve Paquet

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    R-iLACCO1
    

    Use

    AAV

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTRE3G-Bi-gePSI-pGFAP-AcGFP

    Plasmid

    #133733

    Purpose

    Expresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a GFAP promoter.

    Depositor

    Erin Schuman

    Article

    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Inserts

    gePSI-β-chain
    gePSI-α-chain
    AcGFP1
    

    Use

    AAV

    Tags

    murine ornithine decarboxylase - degron (ODC36)

    Expression

    Mammalian

    Promoter

    pGFAP and pTRE3G-Bi

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGFP-HBO1-G485A pc2201

    Plasmid

    #167570

    Purpose

    The human HBO1 is fused to C-terminal GFP of EGFP-C1 (Clontech)

    Depositor

    Cristina Cardoso

    Article

    Heinz et al Epigenetics Chromatin. 2019 Mar 14;12(1)

    Insert

    histone acetyltransferase 1 (KAT7 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    G485A

    Promoter

    CMV

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-actin prom-dsRed-adra2a.1227.shRNA

    Plasmid

    #67880

    Purpose

    expresses dsRed and shRNA to knock down adra2a receptors

    Depositor

    William Wisden

    Article

    Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

    Insert

    dsRed and shRNA to knock down the mouse adra2 receptor
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    chicken beta actin

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pAAV ORANGE Dlg4-HaloTag KI

    Plasmid

    #139656

    Purpose

    Endogenous tagging of PSD95: C-terminal (amino acid position: R721)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and HaloTag donor
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAVTRE3_Clover

    Plasmid

    #135179

    Purpose

    AAV expression of TRE(TRE3G)-driven, Clover expression for fluorescent labling

    Depositor

    Tetsuo Yamamori

    Article

    Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

    Inserts

    Clover
    TRE3G
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    a variant of GFP

    Promoter

    TRE3G

    Available Since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-anti-GFP(LaG17) PAGER(TF)

    Plasmid

    #229997

    Purpose

    Expresses anti-GFP PAGER(TF) (high reversibility) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    IL2SP-Aro6-LaG17-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    V360A/R361A on KORD

    Promoter

    CMV

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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