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pLKO-Cre sgAmotl2 v3

Plasmid

#158602

Purpose

lenti-viral construct with Cas9, Cre recombinbase and U6 driven sgRNA against mouse Amotl2

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Amotl2 sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Sept. 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pTracer_Cr-TRP1

Plasmid

#64881

Purpose

Expresses CrTRP1 in mammalian cells

Depositor

Sebastián Brauchi

Article

Arias-Darraz et al Plant Cell. 2015 Jan;27(1):177-88. doi:

Insert

Transient receptor potential 1

Tags

eGFP

Promoter

CMV

Available Since

June 20, 2016

Availability

Academic Institutions and Nonprofits only
Cas12f-GE ver3.0

Plasmid

#176508

Purpose

Expresses Cas12f-GE in mammalian cells

Depositor

Yong-Sam Kim

Article

Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

Inserts

Cas12f-GE ver3.0
Cas12f-GE ver3.0

Expression

Mammalian

Promoter

U6 and chicken β-actin

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAS_4xEcoLeuT(CUA)_EF1_sfGFP150TAG

Plasmid

#174892

Purpose

amber suppression reporter sfGFP150TAG with EcoLeuT(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG in sfGFP

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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Dynamin-I-K44A-6His/Flag

Plasmid

#32696

Depositor

Robert Lefkowitz

Article

Ahn et al J Biol Chem. 2002 Jul 19;277(29):26642-5

Insert

Dynamin I (Dnm1 Rat)

Tags

Flag and His6

Expression

Mammalian

Mutation

K44A

Promoter

CMV

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
SARS_COV2_ORF9B_HA_BASU

Plasmid

#153981

Purpose

Express Orf9b gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

orf9b

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR SV40 PuroR L3-L2

Plasmid

#62164

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and Puromycin module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Insert

Puromycin

Use

Mule gateway entry vector

Expression

Mammalian

Promoter

SV40

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pJ3 SHP2 CS

Plasmid

#8319

Depositor

Ben Neel

Article

Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

Insert

SHP2 (PTPN11 Human)

Expression

Mammalian

Mutation

C459S, mutation of essential catalytic cysteine

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

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SPLINTR GFP v1 backbone

Plasmid

#179777

Purpose

SPLINTR backbone vector

Depositor

Mark Dawson

Article

Fennell et al Nature. 2022 Jan;601(7891):125-131. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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Sa-dCas9-NLS-3xFLAG/pcDNA3.1

Plasmid

#98041

Purpose

Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interest

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Insert

Sa-dCas9-NLS-3xFLAG

Use

CRISPR

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Mutation

D10A + H557A

Promoter

CMV

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pX330.Pten.b

Plasmid

#66588

Purpose

sgRNA for Pten deletion

Depositor

Wen Xue

Article

Li et al Genome Biol. 2015 May 28;16(1):111.

Insert

Pten deletion (Pten Mouse)

Expression

Mammalian

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only
pAAV_GWRev_Dest

Plasmid

#86954

Purpose

Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. With cre, insert will flip backwards, causing it to turn off (cre-off)

Depositor

Joseph Dougherty

Article

Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-hSOX2 (Plath)

Plasmid

#17965

Depositor

Kathrin Plath

Article

Lowry et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

Insert

SRY (sex determining region Y)-box 2 (SOX2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

N/A

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pAG225 HIPK1 3'UTR mut

Plasmid

#12044

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

HIPK1 3'UTR mut (HIPK1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
pCD5-D/bovine/Nebraska/9-5/2012-HEFs57aED-GCN4-sfGFP-ST

Plasmid

#175020

Purpose

Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

Depositor

Robert de Vries

Article

Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

Insert

D/bovine/Nebraska/9-5/2012

Tags

GCN4-TEV-sfGFP-TwinStrep

Expression

Mammalian

Mutation

s57a esterase knock out mutant

Promoter

CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAGH10 - RlucFluc minus 1 HIV-1

Plasmid

#198224

Purpose

Fluorescence reporters for -1 ribosomal frameshifting in mammalian cells

Depositor

Isha Jain

Article

Baik et al Mol Cell. 2023 Mar 16;83(6):942-960.e9.

Inserts

Renilla luciferase
Firefly luciferase

Expression

Mammalian

Promoter

SV40

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAG177 HIPK1 3'UTR mut

Plasmid

#12035

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N10 3'UTR mut (HIPK1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only

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pCA26-pLenti.CAG.-NLS-dCas13b-NLS-VPR-T2A-HaloTag

Plasmid

#199457

Purpose

Expression of dCas13-VPR in mammalian cells

Depositor

Baohui Chen

Article

Liang et al Nat Commun. 2022 Nov 28;13(1):7329. doi:

Insert

dCas13

Use

Lentiviral

Tags

VPR-T2A-HaloTag

Expression

Mammalian

Mutation

Nuclease-dead H133A and H1058A

Promoter

CAG

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA-CRY2-mCh-superPLDx10-P2A-CIBN-CAAX

Plasmid

#188991

Purpose

A plasmid for dual expression of CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type) and plasma membrane-tagged CIBN

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

PLD

Tags

CAAX, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

F163L, K249R, G328S, G381V, G429D, D480G

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3F-BE3

Plasmid

#113414

Purpose

Expresses hA3F-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3F-BE3 (APOBEC3F Human, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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