We narrowed to 17,816 results for: Met;

1. pSems-leader-mEGFP-MBP-TMD(ALA7)

   Plasmid

   #193051

   Purpose

   Expression of artificial transmembrane protein tagged with mEGFP and MBP at the plasma membrane. Control construct for single molecule co-localization and co-tracking microscopy.

   Depositor

   Jacob Piehler

   Article

   Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

   Insert

   ALA7-TMD
   

   Use

   Tags

   Ig k-chain leader sequence, MBP, and mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-VTKS2-Dreadd-hM3Dq-HA-P2A-tBFP

   Plasmid

   #178707

   Purpose

   AAV vector for Cre-dependent transgene expression of Dreadd-hM3Dq-HA-P2A-tBFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

   Depositor

   Jordane Dimidschstein

   Article

   Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

   Insert

   Dreadd-hM3Dq-HA-P2A-tBFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-CIB1-mRFP1-MP

   Plasmid

   #58367

   Purpose

   For use in light-induced protein inactivation through the interaction with CRY2

   Depositor

   Won Do Heo

   Article

   Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

   Inserts

   CIB1[1-147] (CIB1 Mustard Weed)
   Camk2a[315-478] (Camk2a Rat)
   

   Use

   Tags

   mRFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpdCas9-huTET1CD-T2A-GFP (PX458)

   Plasmid

   #129025

   Purpose

   targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA cloning site
   

   Use

   Tags

   HA-Tag, NLS and T2A-EGFP

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpdCas9-hudTET1CD-T2A-GFP (PX458)

   Plasmid

   #129026

   Purpose

   inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA cloning site
   

   Use

   Tags

   HA-Tag, NLS and T2A-EGFP

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1α1.1-FLPX-rc [Chronos-GFP]

   Plasmid

   #122102

   Purpose

   AAV-mediated expression of Chronos-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a（1.1kb short version)

   Available since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-hGH

   Plasmid

   #182425

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-human growth hormone fusion proteins

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Inserts

   VNp-LZ-mNeongreen-hGH (GH1 Human)
   VNp-LZ
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp) and Vesicle Nucl…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223-H-RAS V12

   Plasmid

   #31201

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   H-Ras (HRAS Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   G12V constitutively active

   Promoter

   Available since

   July 12, 2011

   Availability

   Academic Institutions and Nonprofits only

9. mIFP24-LaminA-C-18

   Plasmid

   #56267

   Purpose

   Localization: Nuclear Envelope, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   LaminA (LMNA Human)
   

   Use

   Tags

   mIFP24

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. Mouse Ism1 with C-terminal Myc-6X-his tag

    Plasmid

    #173046

    Purpose

    Expresses Ism1 in mammalian cells

    Depositor

    Katrin Svensson

    Article

    Jiang et al Cell Metab. 2021 Jul 28. pii: S1550-4131

    Insert

    Ism1 (Ism1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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