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pLminP_Luc2P_RE58

Plasmid

#247035

Purpose

IRF1 pathway reporter; lentiviral expression of IRF1 DNA response element cloned 5' to firefly luciferase. Coexpresses EmGFP to monitor lentiviral transduction.

Depositor

Ramnik Xavier

Publication

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

IRF1

Available since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE2

Plasmid

#90336

Purpose

ATF6 - Unfolded protein response element (UPRE) gene reporter

Depositor

Ramnik Xavier

Publication

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

ATF6

Available since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

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TFORF2038

Plasmid

#144327

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Publication

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TP53BP1 (TP53BP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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DDX24

Plasmid

#155512

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DDX24 (DDX24 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Neo_h53BP1_gRNA_D

Plasmid

#110300

Purpose

Expresssion of Cas9-T2A-neomycin resistant gene and a gRNA targeting exon 10 of human 53BP1

Depositor

Chris Kok-Lung Chan

Publication

Addis Jones et al Nat Commun. 2019 Jun 28;10(1):2861. doi:

Insert

p53-binding protein 1 (TP53BP1 Human)

Expression

Mammalian

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-RAMP4-IRES-Puromycin

Plasmid

#134863

Purpose

Expresses EGFP-tagged endoplasmic reticulum marker

Depositor

David Andrews

Publication

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

RAMP4 (SERP1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pmiR-report-hVEGFA

Plasmid

#112553

Purpose

3'UTR reporter assay

Depositor

Shusheng Wang

Publication

Zhou et al Mol Ther. 2016 Oct;24(10):1823-1835. doi

Insert

VEGFA (VEGFA Human)

Use

Luciferase

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAct:MS2-p65-HSF

Plasmid

#78902

Purpose

Expresses MS2-p65-HSF under actin promoter for SAM CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Publication

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

MS2-p65-HSF

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available since

Sept. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pET11a-CA(A14C, E45C, A92E)

Plasmid

#79046

Purpose

Bacterial vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Publication

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

CA (A14C, E45C, A92E)

Expression

Bacterial

Mutation

A14C, E45C, A92E mutations in HIV-1 CA protein

Promoter

T7

Available since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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CSII-IDR2-rhesusTRIM5-FOS

Plasmid

#79059

Purpose

Express rhesus TRIM5 in mammalian cells

Depositor

Wesley Sundquist

Publication

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

rhesusTRIM5-FOS

Tags

OneSTrEP-FLAG

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIM5alpha(human)

Plasmid

#79034

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Publication

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(human) (TRIM5 Human)

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

polyhedrin

Available since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

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CAGhCBFA2T2

Plasmid

#84363

Purpose

FLAG-HA tagged human CBFA2T2

Depositor

Danny Reinberg

Publication

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

CBFA2T2 (CBFA2T2 Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CAG

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pISH-Msx1

Plasmid

#84313

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Msx1. Not for gene expression.

Depositor

Peter Mombaerts

Publication

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Msx1 (Msx1 Mouse)

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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CSII-IDR2-owlTRIMCyp-FOS

Plasmid

#79067

Purpose

Express owl monkey TRIMCyp in mammalan cells

Depositor

Wesley Sundquist

Publication

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5Cyp (owl monkey)-FOS

Tags

OneSTrEP-FLAG

Expression

Mammalian

Promoter

CMV

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAav-MCS-PQS1-3xFLAG

Plasmid

#84883

Purpose

rAAV-based template for genome engineering of protein C-termini containing PQS1 and 3xFLAG tags and a selection cassette

Depositor

Sven Eyckerman

Publication

Vandemoortele et al Sci Rep. 2016 Jun 6;6:27220. doi: 10.103

Insert

Multicloning sites and selection cassette

Use

AAV

Promoter

PGK

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-En2

Plasmid

#84310

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for En2. Not for gene expression.

Depositor

Peter Mombaerts

Publication

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

En2 (En2 Mouse)

Available since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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J23117-mRFP1-331Bb

Plasmid

#78280

Purpose

mRFP1 behind constitutive promoter J23117 in pSEVA331Bb backbone

Depositor

Tom Ellis

Publication

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

J23117-mRFP1

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pLux02

Plasmid

#78282

Purpose

mRFP1 expressed behind an AHL-inducible construct

Depositor

Tom Ellis

Publication

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

AHL inducible mRFP1

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pCFD3-MS2tail-SAM

Plasmid

#78905

Purpose

Vector for expressing a single sgRNA with SAM MS2 tail, under U6:3 promoter

Depositor

Norbert Perrimon

Publication

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Type

Empty backbone

Use

CRISPR

Expression

Insect

Available since

Aug. 27, 2016

Availability

Academic Institutions and Nonprofits only

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PML-ER-pBabe

Plasmid

#73556

Purpose

vector encoding a fusion protein between PML-IV and ER (estrogen receptor)

Depositor

Gerardo Ferbeyre

Publication

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

PML-IV (PML Human)

Use

Retroviral

Tags

ER

Expression

Mammalian

Promoter

SV40

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only