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phH1-gRNA

Plasmid

#53186

Purpose

Expresses the S. pyogenes sgRNA from the H1 promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

human H1

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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pmU6-gRNA

Plasmid

#53187

Purpose

Expresses the S. pyogenes sgRNA from the mouse U6 promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

mouse U6

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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lentiGuide-mCherry

Plasmid

#217005

Purpose

lentiviral plasmid for gRNA cloning with selectable mCherry expression

Depositor

Deepta Bhattacharya

Article

D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pPAmCherry-a-tubulin

Plasmid

#31930

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

Insert

PAmCherry1

Tags

a-tubulin

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pMP71-BFP

Plasmid

#141355

Purpose

pMP71 expression vector containing BFP

Depositor

Ton Schumacher

Article

Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

Insert

tagBFP

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSAM_Cam

Plasmid

#91570

Purpose

Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Lac promoter expresses transposase. Chloramphenicol resistance on backbone.

Depositor

Harry Mobley

Article

Bachman et al MBio. 2015 Jun 9;6(3):e00775. doi: 10.11

Type

Empty backbone

Expression

Bacterial

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only
mTagBFP2-MannII-N-10

Plasmid

#55309

Purpose

Localization: Mannosidase/Golgi, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

MannII

Tags

mTagBFP2

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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BmTyr-Flag

Plasmid

#220779

Purpose

non-targeted BmTyr throughout the cytoplasm

Depositor

Itaru Hamachi

Article

Zhu et al J Am Chem Soc. 2024 Mar 20;146(11):7515-

Insert

Bacillus megaterium Tyrosinase

Tags

Flag tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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lentiGuide-tagBFP

Plasmid

#217007

Purpose

lentiviral plasmid for gRNA cloning with selectable tagBFP expression

Depositor

Deepta Bhattacharya

Article

D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA-eGFP-Reporter

Plasmid

#60719

Purpose

Expresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP reporter.

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

gRNA (5'-AAAGGTCGAGAAACTGCAAA-3')

Use

CRISPR

Expression

Mammalian

Promoter

U6 and mouse U6

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pPaxillin-PSmOrange

Plasmid

#31923

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Tags

Paxillin

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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prsTagRFP-C1

Plasmid

#31926

Depositor

Vladislav Verkhusha

Article

Subach et al Chem Biol. 2010 Jul 30;17(7):745-55.

Insert

rsTagRFP

Expression

Mammalian

Mutation

F84W/I125L/N148A/S165G/F181L/K189N/Y200F compared…

Promoter

CMV

Available Since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

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pVimentin-PSmOrange

Plasmid

#31922

Depositor

Vladislav Verkhusha

Article

Subach et al Nat Methods. 2011 Jul 31;8(9):771-7. doi

Insert

PSmOrange

Tags

Vimentin

Expression

Mammalian

Mutation

S21T, Q63L, F100Y, L125M, K166R, P192S in PSmOran…

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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PEG-Actinin-M-sstFRET

Plasmid

#61100

Purpose

Expression of α-actinin with internal sstFRET inserted to measure cytoskeletal stress in vivo

Depositor

Fred Sachs

Article

Meng et al J Cell Sci. 2011 Jan 15;124(Pt 2):261-9.

Insert

α-actinin-sstFRET

Expression

Mammalian

Mutation

sstFRET inserted at position 300

Promoter

CMV

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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pMP71-Kaede

Plasmid

#141352

Purpose

pMP71 expression vector containing Kaede

Depositor

Ton Schumacher

Article

Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

Insert

Kaede

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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VV063: 1xCox8 - superecliptic pHluorin in fck

Plasmid

#58500

Purpose

Expresses the pH sensor superecliptic pHluorin in the mitochondria of mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

1xCox8 - Superecliptic pHluorin

Use

Lentiviral

Expression

Mammalian

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-ZO1-C-14

Plasmid

#55335

Purpose

Localization: Tight Junctions, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

ZO1 (TJP1 Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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VV064: 1xCox8 - ratiometric pHluorin in fck

Plasmid

#58502

Purpose

Expresses the pH sensor ratiometric pHluorin in the mitochondria of mammalian cells

Depositor

Adam Cohen

Article

Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

Insert

1xCox8 - Ratiometric pHluorin

Use

Lentiviral

Expression

Mammalian

Promoter

a-CamKII

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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LV-Floxed Luc

Plasmid

#60622

Purpose

Expresses a conditional luciferase cassette dependent on Cre mediated recombination

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

Floxed Luciferase

Use

Cre/Lox, Lentiviral, and Luciferase

Promoter

human UbC

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pMP71-Katushka

Plasmid

#141351

Purpose

pMP71 expression vector containing Katushka

Depositor

Ton Schumacher

Article

Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

Insert

Katushka

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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