We narrowed to 5,108 results for: apoe

1. Zebrafish-gRNA-0001

   Plasmid

   #42241

   Purpose

   gRNA targeted to zebrafish gene apoea

   Depositor

   Keith Joung

   Article

   Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   gRNA-apoea (apoea Zebrafish)
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pCDNA5/FRT/TO–γ-tubulinWT-RNAi-resistant-GFP

   Plasmid

   #205603

   Purpose

   Tet-inducible expression of WT gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion

   Depositor

   Tarun Kapoor

   Article

   Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410

   Insert

   γ-tubulinWT-RNAi-resistant-GFP
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   G2228A, C2231T, C2237T, G2240A

   Promoter

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA5/FRT/TO–γ-tubulinΔGTP-RNAi-resistant-GFP

   Plasmid

   #205604

   Purpose

   Tet-inducible expression of N229A gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertion

   Depositor

   Tarun Kapoor

   Article

   Berman et al J Cell Biol. 2023 Mar 6;222(3):e20220410

   Insert

   γ-tubulinΔGTP-RNAi-resistant-GFP
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   G2228A, C2231T, C2237T, G2240A, N229A

   Promoter

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-Sa-gRNA-puro

   Plasmid

   #191652

   Purpose

   Lentiviral expression of puromycin resistance gene and S. aureus scrambled non-targeting gRNA ; useful for changing gRNA by mutagenesis.

   Depositor

   Moshe Szyf

   Article

   Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

   Insert

   S. aureus gRNAscr
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-dLbCpf1-BE-YE2

   Plasmid

   #154146

   Purpose

   Expresses dLbCpf1-BE-YE2(dLbCas12a-BE-YE2) in mammalian cells.

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

   Insert

   dLbCpf1-BE-YE2 (dCas12a-BE-YE2)
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   APOBEC1(W90Y+R132E), dLbCpf1(D832A+E925A+D1148A)

   Promoter

   CMV

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-dLbCpf1-BE-YEE

   Plasmid

   #154148

   Purpose

   Expresses dLbCpf1-BE-YEE(dLbCas12a-BE-YEE) in mammalian cells.

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

   Insert

   dLbCpf1-BE-YEE (dCas12a-BE-YEE)
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   APOBEC1(W90Y+R126E+R132E), dLbCpf1(D832A+E925A+D1…

   Promoter

   CMV

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJ414-PCY1:S495A

   Plasmid

   #98151

   Purpose

   Expresses PCY1:S495A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1:S495A
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Changed serine 495 to alanine

   Promoter

   T7

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJ414-PCY1:W603A

   Plasmid

   #98153

   Purpose

   Expresses PCY1:W603A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1:W603A
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Changed tryptophan 603 to alanine

   Promoter

   T7

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJ414-PCY1:H695Q

   Plasmid

   #98154

   Purpose

   Expresses PCY1:H695Q from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

   Depositor

   James Naismith

   Article

   Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

   Insert

   Peptide cyclase 1:H695Q
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Changed histidine 695 to glutamine

   Promoter

   T7

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJ414-PCY1:H695A

    Plasmid

    #98155

    Purpose

    Expresses PCY1H695A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1:H695A
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Changed histidine 695 to alanine

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJ414-PCY1:Y696G

    Plasmid

    #98156

    Purpose

    Expresses PCY1:R696G from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

    Depositor

    James Naismith

    Article

    Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

    Insert

    Peptide cyclase 1:Y696G
    

    Use

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Changed tyrosine 696 to glycine

    Promoter

    T7

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pACEBac1-gamma-tubulin-TEV-His6

    Plasmid

    #178067

    Purpose

    Insect cell expression of gamma-tubulin-TEV-His6

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Insert

    gamma-tubulin (TUBG1 Human)
    

    Use

    Tags

    TEV-His6

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-4T-3-cytXlATL2

    Plasmid

    #124063

    Purpose

    Fragment of Xenopus laevis Atlastin (cytATL). This fragment contains the GTPase domain and the three-helix bundle, but lacks the transmembrane segments and the cytosolic tail.

    Depositor

    Tom Rapoport

    Article

    Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

    Insert

    cytXIATL2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pProEx-Htb-cytATL2(R323Q)-6Gly-sfGFP

    Plasmid

    #124066

    Purpose

    E. coli. exp. and purif. of X. laevis cytATL-R232Q fragment fused to GFP in c-terminus.

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    cytATL-R232Q-sfGFP
    

    Use

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    R232Q

    Promoter

    trc

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-mCherry-Cre-blast

    Plasmid

    #179390

    Purpose

    Lentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

    Insert

    mCherry (P2A) Cre recombinase
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 13XLexAop2 IVS ChrimsonR mCherry_tr

    Plasmid

    #111548

    Purpose

    Optogenetics in Drosophila: LexAop2-induced expression of ChrimsonR mCherry

    Depositor

    Vivek Jayaraman

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR_mCherry_trafficked
    

    Use

    Tags

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHAGE2 mCherry-Rtn4a

    Plasmid

    #86683

    Purpose

    Lentivirus to stably express fluorescent human protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    Rtn4a (RTN4 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFastBac Dual α1B/β2B Tubulin

    Plasmid

    #170553

    Purpose

    Encodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.

    Depositor

    Tarun Kapoor

    Article

    Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

    Inserts

    TUBA1B (TUBA1B Human)
    TUBB2B (TUBB2B Human)
    

    Use

    Tags

    AP linker, GS linker (GGSGG), His10 tag, L21 enha…

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFastBac Dual α1B/β3 Tubulin

    Plasmid

    #170552

    Purpose

    Encodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.

    Depositor

    Tarun Kapoor

    Article

    Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

    Inserts

    TUBA1B (TUBA1B Human)
    TUBB3 (TUBB3 Human)
    

    Use

    Tags

    AP linker, GS linker (GGSGG), His10 tag, L21 enha…

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 5XUAS IVS CsChrimson tdtomato_tr

    Plasmid

    #111545

    Purpose

    Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato

    Depositor

    Vivek Jayaraman

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    CsChrimson_tdtomato_trafficked
    

    Use

    Tags

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge