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pEAK-Flag/INPP4B

Plasmid

#24324

Depositor

Lewis Cantley

Article

Gewinner et al Cancer Cell. 2009 Aug 4. 16(2):115-25.

Insert

Inositol Polyphosphate 4-Phosphatase Type II (INPP4B Human)

Tags

Flag and HA

Expression

Mammalian

Mutation

none

Available Since

May 11, 2010

Availability

Academic Institutions and Nonprofits only

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Flag-ECFP-betaPIXa

Plasmid

#15235

Depositor

Rick Horwitz

Article

Mayhew et al J Proteome Res. 2006 Sep . 5(9):2417-23.

Insert

beta-PIXa (Arhgef7 Mouse)

Tags

ECFP and Flag

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only
pBSSK WT p85 alpha flag

Plasmid

#13429

Depositor

Ronald Kahn

Article

Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

Insert

p85 alpha (PIK3R1 Human)

Tags

flag

Expression

Bacterial

Available Since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only

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Flag-SRm160

Plasmid

#11305

Depositor

Phil Sharp

Article

Cheng et al Mol Cell Biol. 2006 Jan . 26(1):362-70.

Insert

SRm160 (SRRM1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HRPT2

Plasmid

#11048

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

HRPT2 (CDC73 Human)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag p38 alpha

Plasmid

#20786

Depositor

Roger Davis

Article

Raingeaud et al J Biol Chem. 1995 Mar 31. 270(13):7420-6

Insert

pCMV5 Flag p38 alpha (Mapk14 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pLV hUbC-dCas9 KRAB-T2A-GFP

Plasmid

#67620

Purpose

Co-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFP

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Insert

humanized dead Cas9 KRAB T2A GFP

Use

CRISPR and Lentiviral

Tags

Flag

Mutation

D10A and H840A

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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plenti-ATF4

Plasmid

#125238

Purpose

ATF4 expression vector

Depositor

Rozita Yarmand

Article

Bagheri-Yarmand et al J Biol Chem. 2015 May 1;290(18):11749-61

Insert

ATF4 (ATF4 Human)

Use

Lentiviral

Tags

Flag

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pT3-EF1aH Yap S127A

Plasmid

#86497

Purpose

Mammalian expression of FLAG-tagged Yap containing S127A mutation

Depositor

Xin Chen

Article

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Yes associated protein 1 (YAP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S127A

Promoter

EF1a

Available Since

Feb. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hPar2

Plasmid

#53228

Purpose

mammalian expression of human Par2

Depositor

Shaun Coughlin

Article

Bohm et al Biochem J. 1996 Mar 15;314 ( Pt 3)(Pt 3)

Insert

Par2 (F2RL1 Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5-F-AF9

Plasmid

#49428

Purpose

Mammalian expression of Flag-tagged human AF9

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

AF-9 (MLLT3 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pLHCX-Flag-mPKM2

Plasmid

#42512

Depositor

Dimitrios Anastasiou

Article

Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

Insert

Pyruvate Kinase M2

Use

Retroviral

Tags

Flag

Promoter

CMV

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pLentiN

Plasmid

#37444

Purpose

Control lentiviral plasmid containing FLAG-HA tags

Depositor

Karl Munger

Article

Spangle et al J Virol. 2012 May 2.

Insert

FLAG-HA

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only
pSO2002

Plasmid

#25842

Depositor

Aziz Sancar

Article

Ozgur et al Biochemistry. 2003 Mar 18. 42(10):2926-3

Insert

human Cryptochrome 2 (CRY2 Human)

Tags

flag, his, and myc

Expression

Mammalian

Available Since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only

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pCMV-MMLVgag-3xNES-ABE7.10-NG

Plasmid

#181753

Purpose

MMLV-Gag fused to 3xNES-ABE7.10-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

Depositor

David Liu

Article

Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

Insert

MMLVgag-3xNES-ABE7.10-NG

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pLL3.7 FLAG-YAP1-TEAD-P-H2B-mCherry

Plasmid

#128327

Purpose

Reporter to evaluate YAP1/TEAD-mediated gene transcription

Depositor

Yutaka Hata

Article

Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

Inserts

H2B mCherry
YAP1 (YAP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pBiex-1 GFPNanobody_SNAP

Plasmid

#82711

Purpose

Expresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.

Depositor

Sivaraj Sivaramakrishnan

Article

Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

Insert

GFP Nanobody

Tags

6xHis Purification tag, FLAG purification tag, an…

Expression

Bacterial and Insect

Promoter

T7 Promoter

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pRRL_SRSF2_P95H_mCherry

Plasmid

#84023

Purpose

expression of SRSF2 mutant in mammalian cells

Depositor

Robert Bradley

Article

Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

Insert

SRSF2 (SRSF2 Human)

Use

Lentiviral

Tags

2TA-mCherry and Flag

Expression

Mammalian

Mutation

P95H

Promoter

PGK

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-MTOR-L1460P

Plasmid

#69006

Purpose

activating MTOR mutation

Depositor

David Sabatini

Article

Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

Insert

MTOR-L1460P (MTOR Human)

Tags

FLAG

Expression

Mammalian

Mutation

change Leucine 1460 to Proline

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCW-Flag-CtIP

Plasmid

#71108

Purpose

mammalian expession of Flag-CtlP

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

CtlP

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Please see Depositor Comments

Promoter

TRE promoter, Tet ON

Available Since

Nov. 11, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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