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pFS_0351_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1

Plasmid

#116963

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pET21a - SodA

Plasmid

#89612

Purpose

Expresses recombinant 6HIS tagged T. cruzi superoxide dismutase A protein in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Superoxide dismutase A

Use

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Mutation

Promoter

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-cMSPdH45

Plasmid

#199767

Purpose

Expresses split-intein fused MSPdH45 for circularization during expression (internal His-Tag)

Depositor

Franz Hagn

Article

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSPdH45

Use

Tags

His6-Tag

Expression

Bacterial

Mutation

internal His-Tag

Promoter

Available since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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Ypet-PBD

Plasmid

#105290

Purpose

To measure Rac or Cdc42 activity by binding and FRET analysis

Depositor

Kenneth Yamada

Article

Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

Insert

Pak1 (PAK1 Human)

Use

Tags

Ypet

Expression

Mammalian

Mutation

aa encoding 65-150

Promoter

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pET22-HT/plsA

Plasmid

#73625

Purpose

Produces Escherichia coli adenylate kinase (PlsA), N-terminal His6 tag

Depositor

James Williamson, T. J. Kappock

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

adenylate kinase (adk Escherichia coli str. K-12 substr. MG1655)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a Cdk2ap1CAN

Plasmid

#178032

Purpose

Expression vector for the purpose of protein purification.

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Nonviral

Tags

6xHIS - Thrombin - MBP- TEV-TRS

Expression

Bacterial

Mutation

Promoter

T7LacI

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pET32a-Spike-S-4P

Plasmid

#165094

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 spike protein site 550-570, 785- 805, 810-830 and 1146 1166 (S SARS-CoV2)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 2, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET28(+)-NudC

Plasmid

#175022

Purpose

Purification of NudC enzyme

Depositor

Jeppe Vinther

Article

Sherwood et al Nature. 2023 Jul;619(7971):811-818. doi:

Insert

nudC

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pET3a-DJ1

Plasmid

#51489

Purpose

Inducible expression of human DJ1 in E.coli

Depositor

Michael J Fox Foundation MJFF

Insert

DJ1 (PARK7 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 31, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pET23a-Mm/HsUBA52

Plasmid

#69560

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Article

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBA52 (UBA52 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pETCON-TNF

Plasmid

#67748

Purpose

Tumor Necrosis Factor Alpha Yeast Display Plasmid

Depositor

Timothy Whitehead

Article

Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

Insert

TNF-Alpha

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pET21b-RL015A

Plasmid

#42134

Depositor

Sheref Mansy

Article

Lentini et al ACS Synth Biol. 2013 Mar 15.

Insert

sfGFP-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Feb. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pYpet-Optop38i

Plasmid

#89749

Purpose

Expression of light-regulated p38 inhibitor, Ypet-fused, wild-type photosensor, inhibitor MK3BD3-13F

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

Optop38i3

Use

Fluorescent protein

Tags

Ypet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a(CTLA4)

Plasmid

#177905

Purpose

Bacterial expression of his10-tagged human CTLA4 Intracellular Domain

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

CTLA4 (aa 183-223 only) (CTLA4 Human)

Use

Tags

10xHis-Thrombin cut site-T7 tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 18, 2024

Availability

Academic Institutions and Nonprofits only

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pET-UbcH10-wt

Plasmid

#8503

Depositor

Joan Ruderman

Article

Bastians et al Mol Biol Cell 1999 Nov;10(11):3927-41.

Insert

UbcH10 (UBE2C Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

June 1, 2005

Availability

Academic Institutions and Nonprofits only
pEThT-RvCAHS12

Plasmid

#192468

Purpose

Bacterial expression of RvCAHS12

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS12

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET11b-HA2

Plasmid

#165992

Purpose

Influenza HA ministem with removable HisTag

Depositor

Vesa Hytönen

Article

Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

Insert

HA2

Use

Tags

Foldon and HisTag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pET32a-HD39Q

Plasmid

#11514

Depositor

Pamela Bjorkman

Article

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

huntingtin, exon 1, 39 glutamines (HTT Human)

Use

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

huntingtin exon 1 with 39 glutamines, vector m…

Promoter

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pFtnA-Ypet

Plasmid

#98280

Purpose

mammalian expression of FtnA-Ypet

Depositor

Mike Heilemann

Article

Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

Insert

FtnA

Use

Tags

Ypet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-Tyr-P(11)4

Plasmid

#185784

Purpose

Encodes enzyme tyrosinase linked to P(11)4 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

Tyr-P(11)4

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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