We narrowed to 9,995 results for: pET

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pET-28a (+)-hTFIIB

Plasmid

#171084

Purpose

Expresses human TFIIB in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TFIIB

Depositor

Joan Conaway

Publication

He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

Insert

TF2B (GTF2B Human)

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(+6)

Plasmid

#199160

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Publication

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(+6)

Tags

MGHHHHHHGGA

Mutation

E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

Promoter

T7

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pET-DUET-1-mRhubarb719-HO

Plasmid

#141200

Purpose

Expresses mRhubarb719 fluorescent protein and heme oxygenase

Depositor

Elad Firnberg

Publication

Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

Inserts

mRhubarb719
heme oxygenase

Tags

6x-His

Expression

Bacterial

Mutation

R134H, Y198S, T202Y, V203I, W309R, Q310A compared…

Promoter

T7

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pET-Gate2 ccdB

Plasmid

#117116

Purpose

Destination vector

Depositor

Stephan Gruber

Publication

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Type

Empty backbone

Use

Backbone for golden gate assembly for gene target…

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET-PesaR-Clux

Plasmid

#47658

Purpose

PesaR-C promoter, luxCDABE, and terminator in pET17b(AmpR)

Depositor

Cynthia Collins

Publication

Shong et al ACS Synth Biol. 2013 Jul 23.

Insert

PesaR-C

Use

Synthetic Biology

Expression

Bacterial

Mutation

Additional esa box between -35 site and the origi…

Promoter

PesaR-C

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only
pET-24d-Flag-SKP1

Plasmid

#213690

Purpose

Expresses human SKP1 in bacteria cells

Depositor

Michele Pagano

Publication

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1 (SKP1 Human)

Tags

Flag

Expression

Mammalian

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET-deSpCas9-VP64-6xHis

Plasmid

#92117

Purpose

Expression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

dead/inactive eSpCas9-NLS-3xFLAG-VP64

Use

CRISPR

Tags

3xFLAG, 6xHis, NLS, and VP64

Expression

Bacterial

Mutation

D10A, H840A, K848A, K1003A, R1060A

Promoter

T7

Available since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-(pos14)GFP

Plasmid

#89252

Purpose

Expresses GFP with +14 theoretical charge in E.Coli

Depositor

David Liu

Publication

Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

Insert

His6-(pos16)-GFP

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pEThT-CAHS1

Plasmid

#90027

Purpose

To express His-tagged CAHS1 in bacteria

Depositor

Takekazu Kunieda

Publication

Yamaguchi et al PLoS One. 2012;7(8):e44209.

Insert

CAHS1

Tags

His

Expression

Bacterial

Promoter

T7

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET23a-HsRPS27a

Plasmid

#69561

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Publication

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

RPS27A (RPS27A Human)

Expression

Bacterial

Promoter

T7

Available since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pET23a-Mm/HsUBA52

Plasmid

#69560

Purpose

Bacterial expression; in vitro transcription/translation (T7)

Depositor

Jorge Eduardo Azevedo

Publication

Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

Insert

UBA52 (UBA52 Human)

Expression

Bacterial

Promoter

T7

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pET28a-Hisx6-Sirt6

Plasmid

#20277

Depositor

Leonard Guarente

Publication

Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

Insert

Sirt6 (Sirt6 Mouse)

Tags

Hisx6

Expression

Bacterial

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only
pET-Neq2X

Plasmid

#182365

Purpose

Expresses Neq2X DNA polymerase

Depositor

Morten Norholm

Publication

Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

Insert

Neq2X

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pET-X(5010)B1

Plasmid

#168019

Purpose

Expresses X(5010)B1-6xHis in bacterial cells

Depositor

David Liu

Publication

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

X(5010)B1-6xHis

Tags

Hisx6

Expression

Bacterial

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET-X(4130)B1

Plasmid

#168018

Purpose

Expresses X(4130)B1-6xHis in bacterial cells

Depositor

David Liu

Publication

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

X(4130)B1-6xHis

Tags

Hisx6

Expression

Bacterial

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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PET-3a-chpBK

Plasmid

#155201

Purpose

To express recombinant ChpBK protein

Depositor

Guanzheng Luo

Publication

Zhang et al Sci Adv. 2019 Jul 3;5(7):eaax0250. doi:

Inserts

chpS-chpBK-6×His-Tag
GyrA-CBD-10xHis-Tag

Tags

6xHis and 10xHis

Expression

Bacterial

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pET-gSTEP1

Plasmid

#159376

Purpose

Expresses gSTEP1 in E. coli

Depositor

Roberto Chica

Publication

Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

Insert

gSTEP1

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pET-NEMOL329P

Plasmid

#11969

Depositor

Jon Ashwell

Publication

Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

Insert

NEMOL329P (IKBKG Human)

Tags

His

Expression

Bacterial

Mutation

Leucine329 to Proline

Available since

July 13, 2006

Availability

Academic Institutions and Nonprofits only
pSG058

Plasmid

#214261

Purpose

PaFtsH1-6xHis cloned between XbaI/NheI in pET-28a(+)

Depositor

Ute Romling

Publication

Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

Insert

Core genome PaFtsH1 proteinase from Pseudomonas aeruginosa SG17M

Tags

6xHis-tag

Expression

Bacterial

Promoter

T7 promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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S019

Plasmid

#174552

Purpose

pET-iBclxL-v3, His tag for affinity purification

Depositor

Bruno Correia

Publication

Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:

Insert

iBclxL-v3, Bclxl inhibitor resistant protein

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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