We narrowed to 8,637 results for: pcDNA3

1. pcDNA3-ΔN-RNMT

   Plasmid

   #112708

   Purpose

   Expresses FLAG-NES-RNMT 121-476 D203A ("ΔN-RNMT") in mammalian cells. Overexpression inhibits cytoplasmic cap methylation.

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   RNMT (RNMT Human)
   

   Tags

   FLAG and HIV Rev nuclear export signal (NES)

   Expression

   Mammalian

   Mutation

   deleted amino acids 2-120, mutated active-site D2…

   Promoter

   CMV

   Available Since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcdna3mciap1 mut

   Plasmid

   #11476

   Depositor

   Jon Ashwell

   Article

   Conze et al Mol Cell Biol. 2005 Apr . 25(8):3348-56.

   Insert

   mciap1mut (Birc2 Mouse)
   

   Expression

   Mammalian

   Mutation

   582 to alanine

   Available Since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1-VinculinI997A_TurboID_Venus

   Plasmid

   #200274

   Purpose

   Expressing actin-binding-deficient vinculin mutant with co-localization labeler TurboID and fluorescence reporter Venus

   Depositor

   Brenton Hoffman

   Article

   Tao et al Dev Cell. 2023 Mar 10:S1534-5807(23)0007

   Insert

   VinculinI997A_TurboID_Venus
   

   Expression

   Mammalian

   Mutation

   change isoleucine to alanine at 997 of vinculin

   Promoter

   T7

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Barkor (FL)

   Plasmid

   #24394

   Depositor

   Qing Zhong

   Article

   Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

   Insert

   Barkor (ATG14 Human)
   

   Tags

   His and Myc

   Expression

   Mammalian

   Available Since

   April 1, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.1/Hygro(-)-B2-cT

   Plasmid

   #104355

   Purpose

   Expresses human beta 2 integrin tension sensor

   Depositor

   Timothy Springer, Pontus Nordenfelt

   Article

   Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10

   Insert

   Human integrin b2 with tension sensor (ITGB2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1_RapACR_T111C_N248Q-EYFP

   Plasmid

   #119197

   Purpose

   Mammalian expression plasmid encoding fast anion channelrhodopsin mutant RapACR_T111C_N248Q

   Depositor

   John Spudich

   Insert

   Anion channelrhodopsin RapACR fast mutant T111C_N248Q
   

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   changed threonine 111 to cysteine and asparagine …

   Promoter

   CMV

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)mGAT1YFP8

   Plasmid

   #41669

   Depositor

   Henry Lester

   Article

   Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

   Insert

   Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   “Monomerizing” YFP A206K mutation; 8 C-terminal-m…

   Promoter

   CMV

   Available Since

   Jan. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1 GW-mCit-PA

   Plasmid

   #113449

   Purpose

   ProteinA-mCitrine Gateway shuttle vector for C-terminal fusions

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Type

   Empty backbone

   Use

   Gateway shuttle vector

   Tags

   mCitrine-ProteinA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. tubulin-ReNL_pcDNA3

   Plasmid

   #89530

   Purpose

   Red color variant of bright luminescent protein, fused to b-tubulin

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   ReNL
   

   Tags

   b-tubulin

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-mNox1

    Plasmid

    #58340

    Purpose

    expresses mouse Nox1 in mammalian cells

    Depositor

    Karl-Heinz Krause, Botond Banfi

    Article

    Banfi et al J Biol Chem. 2003 Feb 7;278(6):3510-3. E

    Insert

    Mus musculus NADPH oxidase 1 alpha (Nox1 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-hPMCA4b

    Plasmid

    #47595

    Depositor

    Emanuel Strehler

    Insert

    PMCA4b (ATP2B4 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1+-PAFAH1B1_R22-

    Plasmid

    #92431

    Purpose

    mammalian expression of PAFAH1B1 R22 mutant. The protein is truncated and non functional (negative control).

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

    Insert

    PAFAH1B1 (PAFAH1B1 Human)
    

    Expression

    Mammalian

    Mutation

    R22 (stop codon in position 22)

    Promoter

    CMV

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-IL-2 signal_rabbit Fc

    Plasmid

    #128031

    Purpose

    Mammalian expression vector with N terminal IL-2 signal peptide and rabbit Fc

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Tags

    IL-2 signal peptide and rabbit Fc

    Expression

    Mammalian

    Available Since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-mGnptg

    Plasmid

    #78106

    Purpose

    Mammalian expression of mouse Gnptg with C-terminal His tag

    Depositor

    Thomas Braulke

    Article

    Encarnacao et al J Biol Chem. 2011 Feb 18;286(7):5311-8.

    Insert

    Gnptg (Gnptg Mouse)
    

    Tags

    His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

15. NF-155 pcDNA3

    Plasmid

    #197290

    Purpose

    Plasmid expressing an antigen targeting the Plasmid expressing an antigen targeting Neurofascin-155

    Depositor

    Peter Brophy

    Article

    Collinson et al Glia. 1998 May;23(1):11-23

    Insert

    Pan-Neurofascin (extracellular) (Nfasc Rat)
    

    Expression

    Mammalian

    Available Since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-EphB4-Fc

    Plasmid

    #200986

    Purpose

    Mammalian expression plasmid for soluble EphB4 fused to IgG1 Fc

    Depositor

    Erik Procko

    Article

    Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

    Insert

    EphB4 (EPHB4 Human)
    

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Soluble ectodomain of EphB4

    Promoter

    CMV

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)_UGControl

    Plasmid

    #133875

    Purpose

    Expresses UGControl in mammalian cells

    Depositor

    Sumio Terada

    Article

    Nakai et al Microscopy (Oxf). 2019 Jul 2. pii: 55252

    Insert

    UtrCH (UTRN Human)
    

    Tags

    flexible linker - mEGFP

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(-)_CMVtrunc_877

    Plasmid

    #105828

    Purpose

    A modified pcDNA3.1- vector with reduced expression activity of a truncated CMV promoter

    Depositor

    Harald Janovjak

    Article

    Kainrath et al Angew Chem Int Ed Engl. 2017 Apr 10;56(1

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)-sapphireAKAR(T/A)

    Plasmid

    #118465

    Purpose

    Negative-control mutant for sapphireAKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    sapphireAKAR(T/A)
    

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3HA-ErbB4CTF

    Plasmid

    #17803

    Depositor

    Marius Sudol

    Article

    Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

    Insert

    ErbB4 (ERBB4 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Carboxy-terminal fragment, amino acids 676-1292

    Available Since

    May 9, 2008

    Availability

    Academic Institutions and Nonprofits only