We narrowed to 1,524 results for: piggyBac

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pJL306

Plasmid

#243703

Purpose

A piggyBac vector containing the NDUFB9 coding sequence (CDS) flanked by the 5' and 3' UTRs of the Actb gene

Depositor

Jonathan Weissman

Publication

Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

Insert

ACTB 5'UTR-NDUFB9 CDS-ACTB 3'UTR (NDUFB9 Human)

Expression

Mammalian

Promoter

EF1a

Available since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-Puro-mCherry-RAB7-S72A

Plasmid

#221556

Purpose

Expresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

Depositor

Shawn Ferguson

Publication

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

Rab7A (RAB7A Human)

Use

Piggybac

Tags

mCherry

Expression

Mammalian

Mutation

Change serine 72 to alanine

Promoter

EF1a

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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Pb459-mU6-sgRNA-EF1a-PuroR

Plasmid

#195507

Purpose

piggybac vector expressing non-targeting control sgRNA cloned using BlpI and BstXI sites

Depositor

Joanna Wysocka

Publication

Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Puromycin

Use

CRISPR; Piggybac

Expression

Mammalian

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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PB-Blast-Dest-LifeAct-mRuby

Plasmid

#194907

Purpose

To generate LifeAct-mRuby expressing stable cell lines with piggybac transposase co-expression and blastocidin selections.

Depositor

Clare Buckley

Publication

Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

Insert

LifeAct

Use

Mouse Targeting

Tags

mRuby

Expression

Mammalian

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Antares2-N1

Plasmid

#120869

Purpose

Piggybac transposon plasmid for live animal optical imaging

Depositor

Jordan Green

Publication

Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

Insert

Akaluc

Expression

Mammalian

Promoter

CMV Promoter

Available since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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KA2944_pPB-TREtight-Esrrb-FLAG-InO

Plasmid

#124179

Purpose

piggyBac-based Dox-inducible expression of Esrrb-FLAG

Depositor

Hans Schöler

Publication

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Esrrb-FLAG-IRES-H2B-mKO2 (Esrrb Mouse)

Expression

Mammalian

Promoter

tetO-CMVmin

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-FullLength-TET1-CXXC/LLAA

Plasmid

#124399

Purpose

Full-length mutant TET1 (L897A/L900A), missing zinc finger domain (585-624), used to PiggyBAC rescue the function of TET1/2 DKO cells

Depositor

Shaun Cowley

Publication

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

FLAG-FullLength-TET1 CXXC zinc finger deletion (585-624) and L897A/L900A mutations (TET1 Human)

Tags

Flag

Expression

Mammalian

Mutation

CXXC zinc finger deletion (aa 585-624) and L897A/…

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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KA2896_pPB-TREtight-Tkmin-cHA-IRESH2BmKO2

Plasmid

#124175

Purpose

piggyBac-based Dox-inducible expression (very weak)

Depositor

Hans Schöler

Publication

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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KA2895_pPB-TREtight-TATAmin-cHA-IRESH2BmKO2

Plasmid

#124174

Purpose

piggyBac-based Dox-inducible expression (weak)

Depositor

Hans Schöler

Publication

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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KA0718_pPB-TREtight-cHA-IRESVenus

Plasmid

#124171

Purpose

piggyBac-based Dox-inducible expression

Depositor

Hans Schöler

Publication

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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KA1265_pPB-gPou5f1_3.5kb-UBCminEGFP-IP

Plasmid

#124180

Purpose

piggyBac-based reporter (EGFP) driven by the Pou5f1 enhancer

Depositor

Hans Schöler

Publication

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

EGFP-IRES-Pac, Pou5f1 enhancer

Use

Reporter

Promoter

UBCmin

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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XZ066-(PB)EF1a-LIDARST-CCR6V2-IRESv0-T2A-PuroR-P2A-BFP

Plasmid

#233418

Purpose

PiggyBac donor plasmid with CCR6-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES

Depositor

Xiaojing Gao

Publication

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available since

June 13, 2025

Availability

Academic Institutions and Nonprofits only

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pPB.DEST-ITGB1(YYFF)-mRuby2

Plasmid

#215449

Purpose

Piggybac expression vector with integrin beta1 NPxY mutant (YYFF) tagged with mRuby2

Depositor

Johanna Ivaska

Publication

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Transposon-based stable expression

Tags

mRuby2

Expression

Mammalian

Mutation

Mutations in the NPxY sites Y783F, Y795F; Silent …

Promoter

CAG

Available since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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XZ079 (PB)EF1a-CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Plasmid

#233435

Purpose

PiggyBac donor plasmid with CCR6-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES

Depositor

Xiaojing Gao

Publication

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

CCR6-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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LSM042 (PB)EF1a-AVPR-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Plasmid

#233426

Purpose

PiggyBac donor plasmid AVPR2-V2-ADAR2dd(E488Q,T501A) and ARRB2-tdMCP separated by EMCV IRES

Depositor

Xiaojing Gao

Publication

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

AVPR-ADARdd-P2A-PuroR-IRES-ARRB2-MCP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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LSM061 (PB)SFFV-LIDARsensor(mcherry-BFP) (hygR)

Plasmid

#233427

Purpose

PiggyBac donor plasmid with SFFV driven LIDAR reporter RNA (mCherry as marker, TagBFP as output)

Depositor

Xiaojing Gao

Publication

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

LIDARsensor(mcherry-BFP)

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ054 (PB)SFFV-LIDARsensor (hygR)

Plasmid

#233419

Purpose

PiggyBac donor plasmid with SFFV driven LIDAR reporter RNA (mCherry as marker, EGFP as output) for stable integration

Depositor

Xiaojing Gao

Publication

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

mCherry-SGc(stem-1(3xMS2))cSG-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-HALO-hLRRK2 (LIR1 mutant WEVL -> AEVA)

Plasmid

#229742

Purpose

Expresses Halo tagged human LRRK2 with LIR1 point mutations in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Publication

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

LRRK2 (LRRK2 Human)

Tags

Halo

Expression

Mammalian

Mutation

LIR1 mutant WEVL -> AEVA

Promoter

EF1A

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-HALO-hLRRK2 (LIR2 mutant WTFI -> ATFA)

Plasmid

#229743

Purpose

Expresses Halo tagged human LRRK2 with LIR2 point mutations in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Publication

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

LRRK2 (LRRK2 Human)

Tags

Halo

Expression

Mammalian

Mutation

LIR2 mutant WTFI -> ATFA

Promoter

EF1A

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-HALO-hLRRK2 (LIR1+2 mutant WEVL -> AEVA / WTFI -> ATFA)

Plasmid

#229744

Purpose

Expresses Halo tagged human LRRK2 with LIR1 and LIR2 point mutations in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Publication

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

LRRK2 (LRRK2 Human)

Tags

Halo

Expression

Mammalian

Mutation

LIR1+2 mutant WEVL -> AEVA / WTFI -> ATFA

Promoter

EF1A

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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