We narrowed to 1,267 results for: piggyBac

1. pAS_4xMma PylT(UUA)_FLAG-AcKRS

   Plasmid

   #140010

   Purpose

   Plasmid with 4xMmaP cassette and Mma PylRS for ochre (TAA) suppression; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   AcKRS-3 (pylS Methanosarcina mazei)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   L301M, L305I, Y306F, L309A, C348F

   Promoter

   EF1

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. BII-gR-BtW-eSpCas9

   Plasmid

   #133358

   Purpose

   Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   eSpCas9
   

   Use

   Tags

   Flag-tagged eSpCas9

   Expression

   Mammalian

   Mutation

   eSpCas9 mutations

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. CAG::Gdnf

   Plasmid

   #78209

   Purpose

   piggybac vector for mouse Gdnf expression

   Depositor

   Ivo Lieberam

   Article

   Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

   Insert

   Gdnf (Gdnf Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

4. AAEL007097-Cas9

   Plasmid

   #100705

   Purpose

   The promoter of AAEL007097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

   Depositor

   Omar Akbari

   Article

   Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

   Inserts

   AAEL007097-hspcas9-GFP
   Opie2-dsRed
   

   Use

   CRISPR

   Tags

   GFP and dsRED

   Expression

   Insect

   Mutation

   Promoter

   AAEL007097 and Opie2

   Available since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-CAG-DD-3xFLAG-hSOX17-GS-TagBFP-BGHpA

   Plasmid

   #172226

   Purpose

   Expression vector with Shield-1 stabilizable hSOX17 fused to TagBFP.

   Depositor

   Alexander Meissner

   Article

   Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

   Insert

   SOX17 (SOX17 Human)
   

   Use

   Piggybac transposon

   Tags

   3XFLAG, DD-domain, GS-linker, and TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAEL003877-Cas9

   Plasmid

   #100581

   Purpose

   The promoter of AAEL003877 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

   Depositor

   Omar Akbari

   Article

   Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

   Inserts

   AAEL003877-hspcas9-GFP
   Opie-dsRed
   

   Use

   CRISPR

   Tags

   GFP and dsRED

   Expression

   Insect

   Mutation

   Promoter

   AAEL003877 and Opie2

   Available since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAS_4xMx PylT C41CA_FLAG_Mx PylRS

   Plasmid

   #140013

   Purpose

   Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   Mx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   May 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. U6b-T

   Plasmid

   #117210

   Purpose

   Expresses gRNA in Aedes aegypti

   Depositor

   Omar Akbari

   Article

   Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

   Insert

   u6b promoter-gRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6

   Available since

   Jan. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAS_4xMx PylT C41CA_FLAG_Mx PylRS YA

   Plasmid

   #140014

   Purpose

   Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   Mx1201 PylRS YA (MMALV_RS05520 Methanomethylophilus alvus Mx1201)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Y126A, Y197F

   Promoter

   EF1

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFH2.102_PB-EPN11-MCP_I-T2A-eGFP-PGK-Blast

    Plasmid

    #205547

    Purpose

    Express EPN11-MCP-I-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN11-MCP-I
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. JPF0510

    Plasmid

    #124001

    Purpose

    Encodes pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFH2.103_PB-EPN11-MCP_p6-T2A-eGFP-PGK-Blast

    Plasmid

    #205548

    Purpose

    Express EPN11-MCP-p6-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN11-MCP-p6
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. BII-gR-PtW-eSpCas9

    Plasmid

    #133357

    Purpose

    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    eSpCas9
    

    Use

    Tags

    HA-tagged eSpCas9

    Expression

    Mammalian

    Mutation

    eSpCas9 mutations

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. JPF0512

    Plasmid

    #124003

    Purpose

    Encodes promoter-less expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_spacer-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. JPF0511

    Plasmid

    #124002

    Purpose

    Encodes pHUBC driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_phubc-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. JPF0520

    Plasmid

    #124011

    Purpose

    Encodes pCMV driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pCMV-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MTK0_002

    Plasmid

    #123960

    Purpose

    Encodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB Destination - 2xHS4 - HygroR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAG 442TAA

    Plasmid

    #154779

    Purpose

    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAG 277TAA, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    LaG17-SynNotch
    

    Use

    Tags

    LaG17

    Expression

    Mammalian

    Mutation

    204TAG 442TAA in LaG17-SynNotch, hybrid PylT with…

    Promoter

    EF1

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAEL007584-Cas9

    Plasmid

    #100706

    Purpose

    The promoter of AAEL007584 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL007584-hspcas9-GFP
    Opie2-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Mutation

    Promoter

    AAEL007584 and Opie2

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAS_4xG1 PylT A41AA C55A sfGFP 150TAG

    Plasmid

    #154770

    Purpose

    plasmid with 4xG1 PylT cassette (G1 PylT mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG in GFP reporter, G1 PylT with A41AA and C5…

    Promoter

    EF1

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge