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Plasmid#203312PurposeTo all for PiggyBac gene insertion into GenomeDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only
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p101_CRISPRai_PiggyBac
Plasmid#213777PurposeExpresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.DepositorInsertsVPR-dSaCas9
dSpCas9-KRAB-BFP
zeocin
Tet-On transactivator
UseCRISPRTagsExpressionMutationPromoterEF1a (EF-1-alpha intron a) and Tet_On_TREAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac dCas9-KRAB
Plasmid#191314PurposepiggyBac integration and dox-inducible expression of KRAB-dCas9-HA-tagBFPDepositorInsertsKRAB-dCas9-HA-tagBFP
rtTA3-IRES-PURO
UseCRISPRTagsHA and tagBFPExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac dCas9-VPR
Plasmid#191269PurposepiggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFPDepositorInsertsVPR-dCas9-HA-tagBFP
rtTA3-IRES-PURO
UseCRISPRTagsHA and tagBFPExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
piggyBAC-5xTAPE-1-BlastR
Plasmid#175808PurposeEncodes 5xTAPE-1 construct that can be integrated using piggyBAC systemDepositorInsert5xTAPE-1 construct
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNS35 (multiAsCas12a piggyBac)
Plasmid#217336PurposepiggyBac expression of multiAsCas12aDepositorInsertmultiAsCas12a
UseCRISPRTags6xMycNLS and HA-SV40NLS-P2A-TagBFP2ExpressionMammalianMutationR1226A/E174R/S542R/K548RPromoterCAGAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
piggyBAC-U6-GFP-WPRE
Plasmid#200030PurposepiggyBAC vector backbone with GFP for cloning DNA Ticker Tapes and U6 for pegRNAsDepositorTypeEmpty backboneUseTagsGFPExpressionMammalianMutationPromoterU6, EF1aAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
piggyBAC-H2B FR-MQV
Plasmid#194079PurposeExpresses nuclear-localized FR-MQV in mammalian cellsDepositorInsertFR-MQV
UseTagsHistone H2BExpressionMammalianMutationMethionine 42 to Glutamine, Cystiene 159 to Valin…PromoterCMV7Available sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac-G13-sensor
Plasmid#137809PurposeA FRET sensor for G13 activation, encoded on a single plasmidDepositorInsertGalpha13 sensor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQLL-piggyBac-1_XT
Plasmid#217246PurposeDonor plasmid of piggyBac-1_XT TransposonDepositorInsertTIRs of piggyBac-1_XT transposon
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMV-piggyBac-1_AMi
Plasmid#217236PurposeDonor plasmid of piggyBac-1_AMi TransposonDepositorInsertTIRs of piggyBac-1_AMi transposon
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
piggyBAC-BRD4-Flag
Plasmid#214432PurposeA piggyBAC based plasmid for the stable expression of BRD4 with a flag tag.DepositorInsertBrd4 bromodomain containing 4 (Brd4 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterEF1Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
piggyBAC-H2B mCherry-XL (R125K)
Plasmid#189926PurposeExpresses nuclear-localized mCherry-XL (R125K) in mammalian cellsDepositorInsertmCherry-XL (R125K)
UseTagsH2BExpressionMammalianMutationdiffers from mCherry by R125K, W143S, I161V, Q163…PromoterCMV7Available sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac-TetO-Ascl1-neomycin
Plasmid#176482PurposeTetO-Ascl1 in a PiggyBac vector with neomycin resistanceDepositorInsertAscl1 (Ascl1 Mouse)
UseTagsExpressionMammalianMutationPromoterTetOAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Piggybac-TetO-hNurr1-blast
Plasmid#176485PurposeTetO-hNurr1 in a PiggyBac vector with Blasticidin resistanceDepositorInsertNurr1 (NR4A2 Human)
UseTagsExpressionMammalianMutationPromoterTetOAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
piggybac-EF1-H2AZ-halo
Plasmid#197907Purposesingle-particle tracking for H2AZDepositorInsertH2az2 (H2az2 Mouse)
UseTagsHalotagExpressionMammalianMutationPromoterEF1Available sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCH61 (multiAsCas12a-KRAB piggyBac)
Plasmid#217332PurposepiggyBac expression of multiAsCas12a-KRABDepositorInsertmultiAsCas12a
UseCRISPRTags6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2ExpressionMammalianMutationR1226A/E174R/S542R/K548RPromoterCAGAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
piggyBac CMV-GFP-FRT
Plasmid#92078Purposeproduction of transgenic animal and recombination using flipaseDepositorInsertCMV-eGFP and TK-Neo between FRT3 and FRT
UsePiggybacTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
piggybac-EF1-halo-mTBP
Plasmid#197908Purposesingle-particle tracking for mTBPDepositorInsertTBP (Tbp Mouse)
UseTagsHalotagExpressionMammalianMutationPromoterEF1Available sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCH62 (denAsCas12a-KRAB piggyBac)
Plasmid#217333PurposepiggyBac expression of denAsCas12a-KRABDepositorInsertdenAsCas12a
UseCRISPRTags6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2ExpressionMammalianMutationD908A/E174R/S542R/K548RPromoterCAGAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only