We narrowed to 5,093 results for: puc

1. pUC19-SARS-CoV-2-N-gene-truncated

   Plasmid

   #240635

   Purpose

   pUC19-SARS-CoV-2-N-gene-truncated plasmid is a plasmid that contains the SARS-CoV-2 N-gene and can be used as a positive control in SARS-CoV-2 research or diagnostics assays targeting the N gene.

   Depositor

   Kevin Yehl

   Insert

   SARS-CoV-2-N-gene-truncated (N )
   

   Use

   Synthetic Biology

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19_msfGFP-NPM1-repair-template for U2OS

   Plasmid

   #238246

   Purpose

   For knock-in msfGFP to NPM1 locus

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   NPM1
   

   Use

   CRISPR

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19_msfGFP-NPM1-repair-template for HCT116

   Plasmid

   #238245

   Purpose

   For knock-in msfGFP to NPM1 locus

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   NPM1
   

   Use

   CRISPR

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC57-Homology Arms-BRD4-HaloTag

   Plasmid

   #228575

   Purpose

   CRISPR donor plasmid to tag human BRD4 with Halo tag; for CRISPR-mediated gene insertion

   Depositor

   Marcin Imielinski

   Insert

   Homology Arms-BRD4-HaloTag (BRD4 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC-hU6-crBACH1-array_EF1a-BFP

   Plasmid

   #224787

   Purpose

   BACH1 targeting crRNA array for RfxCas13d expressed from multiple hU6 promoter and reporter BFP protein expressed from EF1a promoter

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Insert

   crBACH1-1, crBACH1-2, crBACH1-3,
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   hU6 and hU6-2xTetO

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC-hU6-crCoV-Mutiplex_EF1a-BFP

   Plasmid

   #224788

   Purpose

   SARS-COV-2 targeting crRNA array for RfxCas13d expressed from single hU6 promoter and reporter BFP protein expressed from EF1a promoter

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Insert

   crCoV20, crCoV21, crCoV24
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   hU6 and hU6-2xTetO

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC57 mini-FLAG-HA-Regnase-3 D252N

   Plasmid

   #222652

   Purpose

   For in vitro transcription of mouse Zc3h12c D252N mutant tagged with FLAG-HA at N-term

   Depositor

   Silvia Monticelli

   Article

   Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

   Insert

   Zc3h12c (Zc3h12c Mouse)
   

   Use

   In vitro transcription

   Tags

   FLAG and HA

   Mutation

   mutated aspartic acid 252 to asparagine to abroga…

   Promoter

   T7

   Available Since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC18T-mini-Tn7T-hph-lacZ

   Plasmid

   #218370

   Purpose

   Empty backbone for lacZ fusions

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   May 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC-tRNAGln-BbsI-sU6-Kan

   Plasmid

   #213016

   Purpose

   Vector for Cloning of multiple gRNAs driven by distinct promoters (tRNA-Gln and synthetic U6)

   Depositor

   Stephanie Roessler

   Insert

   tRNAGln promoter, sU6 promoter, gRNA scaffolds
   

   Promoter

   tRNAGln promoter

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC-ODN-hsRPL3-CterTwStrep-ΔPAM62&56

    Plasmid

    #208645

    Purpose

    Remove PAM region in HomoArms by introduce SNP rs7495557 and rs760194863, to prevent cleavage after success edit. Digest with nt.BspQI and nb.BsrDI to get ssDNA.

    Depositor

    Quanfu Ma

    Insert

    Twin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
    

    Use

    CRISPR; Ssdna donnor preparation for knock-in of…

    Tags

    Twin Strep

    Mutation

    Two SNP (rs749555789, rs760194863) were introduce…

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIG-724pUC19-tNGFR-P2A-EVER2

    Plasmid

    #186105

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    EVER2 (TMC8 Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIG-748pUC19-tNGFR-P2A-AP3B1

    Plasmid

    #186106

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    AP3B1 (AP3B1 Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIG-734pUC19-tNGFR-P2A-PIK3R1

    Plasmid

    #186107

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    PIK3R1 (PIK3R1 Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIG-710pUC19-tNGFR-P2A-IL10R1

    Plasmid

    #186108

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    IL10R1 (IL10RA Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIG-713pUC19-tNGFR-P2A-CD45

    Plasmid

    #186109

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CD45 (PTPRC Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIG-717pUC19-tNGFR-P2A-CD3E

    Plasmid

    #186111

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CD3E (CD3E Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIG-737pUC19-tNGFR-P2A-CD18

    Plasmid

    #186113

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CD18 (ITGB2 Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIG-745pUC19-tNGFR-P2A-CD4

    Plasmid

    #186115

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CD4 (CD4 Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIG-721pUC19-tNGFR-P2A-RhoH

    Plasmid

    #186116

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    RHOH (RHOH Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIG-744pUC19-tNGFR-P2A-CD2

    Plasmid

    #186118

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CD2 (CD2 Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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