We narrowed to 8,858 results for: sgRNA

Showing: 901 - 920 of 8858 results

Results per page:

Sort results by:

AAV-U6-sgRNA-hSyn-mCherry

Plasmid

#87916

Purpose

sgRNA expressing AAV construct with a mCherry reporter driven by hSyn promoter. (replaced the GFP in pX552 from Zhang lab with mCherry)

Depositor

Alex Hewitt

Article

Hung et al Invest Ophthalmol Vis Sci. 2016 Jun 1;57

Insert

mCherry

Use

AAV and CRISPR

Expression

Mammalian

Promoter

hSyn

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-U6-sgRNA-CMV-GFP

Plasmid

#85451

Purpose

Express sgRNA in mammalian cells

Depositor

Hetian Lei

Article

Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

Insert

pCMV-EGFP

Use

AAV

Promoter

pCMV-EGFP

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
plentiCRISPR-v2 Puro EIF2AK3/PERK_sgRNA

Plasmid

#218666

Purpose

sgRNA targeting human EIF2AK3/PERK

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

EIF2AK3 (EIF2AK3 Human)

Use

CRISPR and Lentiviral

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV enAsCas12f-HKRA-sgRNA_v7

Plasmid

#208357

Purpose

Expression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cells

Depositor

Atsushi Hoshino

Article

Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

Insert

enAsCas12f-HKRA

Expression

Mammalian

Mutation

I123H/D195K/D208R/V232A

Promoter

CMV

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-U6-DR-SgRNA-DR-EF1a-mCherry-SV40

Plasmid

#154002

Purpose

Empty SgRNA expression system matching CasRx

Depositor

Hui Yang

Article

He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

Insert

SgRNA expression system

Use

CRISPR

Promoter

U6, EF1a

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEM253 - sgRNA mosTI hygroR

Plasmid

#159827

Purpose

Single copy or array insertion by MosTI using split hygroR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI hygroR

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-U6-sgRNA-BFP-Puro

Plasmid

#229014

Purpose

Perturb Seq lentiviral sgRNA vector

Depositor

Gary Hon

Article

Sivakumar et al Stem Cell Reports. 2025 Dec 9;20(12):102

Type

Empty backbone

Use

Lentiviral

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459-sgRNA4-CTCF-3p-UTR

Plasmid

#195106

Purpose

Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting downstream of the human CTCF 3'UTR. Puromycin selection (2A-fusion).

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

sgRNA against CTCF 3' UTR region

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459-sgRNA3-CTCF-3p-UTR

Plasmid

#195105

Purpose

Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting downstream of the human CTCF 3'UTR. Puromycin selection (2A-fusion).

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

sgRNA against CTCF 3' UTR region

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
OpenCRISPR-1 sgRNA-16nt stem

Plasmid

#221566

Purpose

Express OpenCRISPR-1 sgRNA (16nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

Depositor

Ali Madani

Article

Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

Insert

OpenCRISPR-1 sgRNA 16nt stem loop

Expression

Mammalian

Promoter

U6

Available Since

Aug. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGL3-U6-sgRNA-PGK-puromycin

Plasmid

#51133

Purpose

expresses sgRNA from the U6 promoter.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV enAsCas12f-YHAM-sgRNA_v7

Plasmid

#208356

Purpose

Expression of enAsCas12f-YHAM and sgRNA_v7 in mammalian cells

Depositor

Atsushi Hoshino

Article

Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

Insert

enAsCas12f-YHAM

Expression

Mammalian

Mutation

F48Y/S188H/V232A/E316M

Promoter

CMV

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9(BB)-2A-tomato-TSC2-sgRNA

Plasmid

#196195

Purpose

Editing human TSC2 locus

Depositor

Jürgen Knoblich

Article

Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

Insert

TSC2 (TSC2 Human)

Use

CRISPR

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
sgRNA(MS2) cloning backbone

Plasmid

#61424

Purpose

sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

Plasmid

#217635

Purpose

AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.

Depositor

Martin Kampmann

Article

Ramani et al Nat Neurosci. 2025 Aug 22. doi: 10.1038/

Insert

mU6-sgRNA, Handle sequence, EF1a-mTagBFP2

Use

AAV

Promoter

EF1alpha and mU6

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
OpenCRISPR-1 sgRNA-12nt stem

Plasmid

#221567

Purpose

Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

Depositor

Ali Madani

Article

Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

Insert

OpenCRISPR-1 sgRNA 12nt stem loop

Expression

Mammalian

Promoter

U6

Available Since

Aug. 28, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pMD19-PBBa_J23117-sgRNA-Spr

Plasmid

#190793

Purpose

Template vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.

Depositor

Paul Hudson

Article

Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

Insert

sgRNA (dummy)

Use

Synthetic Biology

Expression

Bacterial

Promoter

BBa_J23117

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-FLEXfrt-SaCas9-U6-sgRNA

Plasmid

#124845

Purpose

Vector for FLP-dependent expression of SaCas9 with gRNA

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

SauCas9 gRNA scaffold

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
FASTHDR-tRNApromo-sgRNA-espCas91_1

Plasmid

#167210

Purpose

Plasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
DNA-PKcs N-terminal sgRNA

Plasmid

#207093

Purpose

pX330 based plasmid for expression of Cas9 and the AGCGGGACTCGGCGGCATGG sgRNA to target the DNA-PKcs locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

AGCGGGACTCGGCGGCATGG

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge