We narrowed to 3,167 results for: tef
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Plasmid#213786PurposeIntegrates at the CAN1 locus the BB-Tv TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassetteDepositorInsertBB-Tv TP-DNAP1
UseSynthetic BiologyExpressionBacterial and YeastAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGAN230
Plasmid#131122Purposereverse yTRAP, RNQ1-aTF reporting on tetR-mKate2DepositorTypeEmpty backboneExpressionYeastPromoterSUP35Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6-mTFP1-His3MX6
Plasmid#69850PurposeC-terminal tagging with mTFP1 using His3MX6 as a selection marker in S. cerevisiae.DepositorInsertmTFP1
ExpressionYeastAvailable sinceApril 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDZ585 pKAN 2x-mCherry
Plasmid#72236PurposeFloxed Kanamycin (G418) selection maker; 3' tagging vector used to create C-terminal fusion proteins with 2x-mCherryDepositorInsert2x-mCherry
ExpressionBacterialAvailable sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomKeima-SpHis5
Plasmid#44847PurposeYeast tagging vector to create gene fusion with yeast optimized mKeima with SpHis5 selectionDepositorInsertyomKeima
ExpressionYeastAvailable sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDB5322
Plasmid#204832PurposeAn integrating plasmid containing both the inducible enotetSW4 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW4 promoterAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCast
Plasmid#200169PurposeE. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozymeDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAV0765
Plasmid#133501PurposeExpression of mCherry targeted to the nucleus with one NLS signalsDepositorInsertpLys3(BstZ17I)-p(tdh1)-NLSSV40-mCherry-terminator(ScADH1)-kanMX
ExpressionYeastAvailable sinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTL032
Plasmid#80928Purpose14x MS2 sense + 14x PP7 antisense with loxP-kanMX-loxPDepositorInsert14xMS2 sense + 14xPP7 antisense, loxP-kanMX-loxP
ExpressionBacterialAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
p41Nat MagicFluor
Plasmid#58563Purposep41Nat carrying both mating type markers buffered by the Cyc1 terminatorDepositorInsertSte2p-Citrine, Cyc1 term, Ste3p-mCherry
ExpressionYeastAvailable sinceNov. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG25-L-IFP-F[1] (clon 3)
Plasmid#52899PurposePlasmid template for PCR of cassettes for HR, used to introduce IFP F[1] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertL-IFP-F[1]
UseVector for homologous recombinationPromoterTEF promoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG32-L-IFP-F[2] (clon 3)
Plasmid#52900PurposePlasmid template for PCR of cassettes for HR, used to introduce IFP F[2] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertL-IFP-F[2]
UseVector for homologous recombinationPromoterTEF promoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-HSV-kanMX6
Plasmid#20775DepositorTypeEmpty backboneUsePcr-based c-terminal taggingExpressionYeastAvailable sinceMarch 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAV0757
Plasmid#133509PurposeExpression of 3GFP tagged CRIB domain to monitor active Cdc42DepositorInsertpHis5(StuI)-p(pak1)-CRIB(gic2aa2-181)-3GFP-terminator(ScADH1)-kanMX
ExpressionYeastAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pADH140
Plasmid#90988PurposeNAT-marked CAS9 expression construct; part 1 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH143-series gRNA expression constructs.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsC. maltosa LEU2 1 of 2
pENO1
Cas9
NAT 1 of 2
UseCRISPRAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-bleMX6-P3nmt1-3FLAG
Plasmid#19351DepositorInsertbleMX6
ExpressionYeastAvailable sinceSept. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-fSNAP-URA
Plasmid#188917PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-fSNAP-URA
ExpressionYeastAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXNgate21-3HA
Plasmid#105078PurposeYeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloningDepositorTypeEmpty backboneTagsNubGExpressionYeastAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNXgate32-3HA
Plasmid#105076PurposeYeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloningDepositorTypeEmpty backboneTags3xHA and NubGExpressionYeastAvailable sinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB3041(gRNA X-3)
Plasmid#73283PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-3DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only