We narrowed to 2,608 results for: ubiquitin

1. pHAGE-FLAG-APEX2-LGALS8

   Plasmid

   #175758

   Purpose

   Lentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS8

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   LGALS8 (LGALS8 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-OTUB1 C91S-pcDNA3.1

   Plasmid

   #118210

   Purpose

   Expresses FLAG-OTUB1 C91S catalytic inactive mutant in mammalian cells

   Depositor

   Cynthia Wolberger

   Article

   Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

   Insert

   OTUB1 (OTUB1 Human)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   changed cysteine 91 to serine

   Promoter

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-myc-hIFITM3-Y20A

   Plasmid

   #58462

   Purpose

   Expresses human IFITM3-Y20A with an N-terminal myc tag in mammalian cells.

   Depositor

   Jacob Yount

   Article

   Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

   Insert

   IFITM3 (IFITM3 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Tyrosine 20 to alanine

   Promoter

   CMV IE

   Available since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pCI HA NEDD4L DD

   Plasmid

   #27001

   Depositor

   Joan Massague

   Article

   Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

   Insert

   NEDD4L (NEDD4L Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C962A

   Promoter

   Available since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3 MDM2 S166D S186D

   Plasmid

   #16236

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

   Insert

   MDM2 (MDM2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Ser166 and Ser186 replaced by Asp

   Promoter

   Available since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pLENTI_RPS2_Keima

   Plasmid

   #127139

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   RPS2_Keima (RPS2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-parkin WT

   Plasmid

   #45969

   Depositor

   Kalle Gehring

   Article

   Trempe et al Science. 2013 May 9.

   Insert

   Parkin (Prkn Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression

   Promoter

   tac

   Available since

   Sept. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. px458_2A_GFP_sgRNA_PELO

   Plasmid

   #127124

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   gRNA PELO (PELO Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac_HT_B_6xHis-TEV-GFP-NBR1

   Plasmid

   #190928

   Purpose

   Plasmid for the expression and purification of GFP labelled NBR1. Internal reference: SMC 914

   Depositor

   Sascha Martens

   Article

   Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

   Insert

   NBR1 (NBR1 Human)
   

   Use

   Tags

   Strep-TEV-GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRS-puro shNEDD4L #1

    Plasmid

    #27016

    Depositor

    Joan Massague

    Article

    Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

    Insert

    shRNA NEDD4L #1 (NEDD4L Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-ALK5 K232R

    Plasmid

    #80878

    Purpose

    mammalian expression of ALK5 K232R

    Depositor

    Aristidis Moustakas

    Article

    Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

    Insert

    ALK5 (TGFBR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K232R (kinase dead)

    Promoter

    CMV

    Available since

    Aug. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. HUWE1-sgRNA-1

    Plasmid

    #86924

    Purpose

    To express sgRNA target HUWE1 gene

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    HUWE1 sgRNA1 (HUWE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

13. HUWE1-sgRNA-2

    Plasmid

    #86925

    Purpose

    To express sgRNA target HUWE1 gene

    Depositor

    Yihong Ye

    Article

    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

    Insert

    HUWE1 sgRNA2 (HUWE1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pCS2-FLAG-hEcto/Tif1g

    Plasmid

    #20902

    Depositor

    Stefano Piccolo

    Article

    Dupont et al Cell. 2005 Apr 8. 121(1):87-99.

    Insert

    Ectodermin (TRIM33 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N-terminal deletion of 37 aminoacids; Ectodermin …

    Promoter

    Available since

    April 22, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA5-FRT/TO-Flag-RNF169

    Plasmid

    #74243

    Purpose

    Expresses RNF169 in mammalian cells

    Depositor

    Daniel Durocher

    Article

    Panier et al Mol Cell. 2012 Aug 10;47(3):383-95. doi:

    Insert

    RNF169 (RNF169 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 20, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pCDNA3-Myc3-Cul3 delta N41

    Plasmid

    #21590

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

    Insert

    cullin 3 (CUL3 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Residues 34-74 deleted from N terminus.

    Promoter

    Available since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pMSCV-puro-Flag-mMcl-1 KR

    Plasmid

    #32983

    Depositor

    Joseph Opferman

    Article

    Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

    Insert

    Mcl-1 (Mcl1 Mouse)
    

    Use

    Retroviral

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    All Lys to Arg

    Promoter

    LTR

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

18. SRa-HA-SUMO1 (Sentrin 1)

    Plasmid

    #17359

    Depositor

    Edward Yeh

    Article

    Kamitani et al J Biol Chem. 1998 Feb 6. 273(6):3117-20.

    Insert

    SUMO1 (SUMO1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRvUBC-mEGFP-NLS

    Plasmid

    #205010

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the ubiquitin c gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP-NLS flanked by 1kbp upstream and downstream of the ubiquitin c gene from R. varieornatus
    

    Use

    Tardigrade expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX6P1-UBE2S-UBD

    Plasmid

    #66713

    Purpose

    Expresses the UBE2S-UBD fusion protein in E. coli

    Depositor

    David Komander

    Article

    Bremm et al Nat Struct Mol Biol. 2010 Aug;17(8):939-

    Inserts

    e2-epf (UBE2S )
    isot (USP5 )
    

    Use

    Tags

    GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only