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pAAV_BiPVe3_dTomato_nlsdTomato

Plasmid

#213940

Purpose

AAV construct expressing dTomato driven by PV+ basket cell-targeting enhancer. Alias: pAAV_WP0003_dTomato_nlsdTomato

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB and AAV1

Insert

dTomato_P2A_nls.dTomato

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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eSrtA(2A-9) pET29b

Plasmid

#75145

Purpose

reprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificity

Depositor

David Liu

Article

Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

reprogrammed sortase A variant

Tags

6x His

Expression

Bacterial

Mutation

S102C A104H E105D K138P K152I N160K K162H T164N K…

Promoter

T7

Available Since

July 28, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-JEDI-2P-Kv-WPRE

Plasmid

#179463

Purpose

Soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector for neuron -specific expression using the promoter hSyn

Depositor

Francois St-Pierre

Article

Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

Has Service

AAV9

Insert

JEDI-2P-Kv

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn1.Twitch2B.WPRE.SV40

Plasmid

#100040

Purpose

AAV expression of Twitch2B driven from hSyn1 promoter. Twitch 2B is a fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Has Service

AAV1

Insert

Twitch2B

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-hG9a

Plasmid

#33025

Depositor

Martin Walsh

Article

Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

Insert

hG9a (EHMT2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2012

Availability

Academic Institutions and Nonprofits only

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pCI-Neo HA-hSki

Plasmid

#10910

Depositor

Bob Weinberg

Article

Sun et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

Insert

Ski (SKI Human)

Tags

HA

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV_BiCHATe27_ChR2_mCherry

Plasmid

#213830

Purpose

AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by cholinergic neuron-targeting enhancer. Alias: pAAV_S9E27_ChR2_mCherry

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB and AAV1

Insert

Channelrhodopsin 2-mCherry

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-NLRP1 codon-optimized

Plasmid

#166833

Purpose

Mammalian codon-optimized NLRP1 with N-terminal and C-terminal HIS tags

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

HIS

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pSICO-Flpo

Plasmid

#24969

Depositor

Tyler Jacks

Article

Young et al Proc Natl Acad Sci U S A. 2010 Jun 1. 10

Insert

Flp recombinase

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only

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pCL20C-ddIPpoI

Plasmid

#49053

Purpose

Expresses the restriction enzyme IPpoI under the control of Shield1

Depositor

Michael Kastan

Article

Goldstein et al Proc Natl Acad Sci U S A. 2013 Sep 30.

Insert

DD-HA-ER-IPpoI

Use

Lentiviral

Available Since

Dec. 17, 2013

Availability

Academic Institutions and Nonprofits only

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AiP12237 - pAAV-AiE0452h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2237)

Plasmid

#191707

Purpose

AI plasmid ALIAS: AiP12237. Expression of SYFP2 (cyan fluorescent protein) in striatal indirect pathway medium spiny neurons (D2-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has Service

AAV PHP.eB

Insert

SYFP2

Use

AAV

Mutation

none

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA HA HP1 beta

Plasmid

#24079

Depositor

Naoko Tanese

Article

Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

Insert

HP1 beta (CBX1 Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
Pmec-18 ced-3 (p15)::nz [TU#806]

Plasmid

#16080

Depositor

Martin Chalfie

Article

Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

Insert

ced-3 (p15) :: nz (ced-3 Nematode)

Expression

Worm

Available Since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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Pmec-18 cz::ced-3 (p17) [TU#807]

Plasmid

#16081

Depositor

Martin Chalfie

Article

Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

Insert

cz:: ced-3 (p17) (ced-3 Nematode)

Expression

Worm

Available Since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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pMSCV-FlagBcl10

Plasmid

#18718

Depositor

Jon Ashwell

Article

Wu et al Proc Natl Acad Sci U S A. 2008 Feb 26. 1

Insert

B cell lymphoma 10 (BCL10 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
ratGlp1-R

Plasmid

#14944

Depositor

Bernard Thorens

Article

Thorens et al Proc Natl Acad Sci U S A. 1992 Sep 15. 8

Insert

Glp1-R (Glp1r Rat)

Expression

Mammalian

Available Since

June 7, 2007

Availability

Academic Institutions and Nonprofits only
pP{ELAV-GeneSwitch}

Plasmid

#83957

Purpose

Expresses conditional RU486-dependent GAL4 protein under control of the elav promotor

Depositor

Haig Keshishian

Article

Osterwalder et al Proc Natl Acad Sci U S A. 2001 Oct 23;98

Insert

fusion protein consisting of the GAL4-DBD, human progesterone receptor-ligand-binding domain, and P65-AD (GAL4, RELA Human, Budding Yeast)

Expression

Insect

Promoter

elav

Available Since

Dec. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pCI-Neo HA-hSnoN

Plasmid

#10908

Depositor

Bob Weinberg

Article

Sun et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

Insert

SnoN (SKIL Human)

Tags

HA

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pTA-attB

Plasmid

#18937

Depositor

Michele Calos

Article

Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

Insert

phiC31 attB

Expression

Bacterial

Available Since

Oct. 17, 2008

Availability

Academic Institutions and Nonprofits only

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Shim_TrpRS_2406.7.1_SHI16564_TP_GFP_pK7fWG2_IVD_FP611

Plasmid

#228381

Purpose

Expresses N-term transit peptide from Sapria himalayana Tryptophan aminoacyl-tRNA synthetase (evm.model.fragScaff_scaffold_2406.7.1/SHI16564RA); to determine S. himalayana TrpRS target localization

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from S. himalayana TrpRS (evm.model.fragScaff_scaffold_2406.7.1/SHI16564RA)

Tags

Green Florescent Protien

Expression

Plant

Available Since

Feb. 26, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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