We narrowed to 11,803 results for: nsf

1. pB PUb-integrase

   Plasmid

   #183966

   Purpose

   piggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent marker

   Depositor

   Eric Marois

   Article

   Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

   Insert

   PhiC31 integrase
   

   Use

   Piggybac insect transgenesis destination vector

   Promoter

   Aedes aegypti Polyubiquitin

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-DYRK3-2A-GFP

   Plasmid

   #118286

   Purpose

   Expresses mouse DYRK3 tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.

   Depositor

   Vance Lemmon

   Article

   Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

   Insert

   dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 (Dyrk3 Mouse)
   

   Use

   AAV

   Tags

   2A peptide and GFP

   Expression

   Mammalian

   Promoter

   CMV with beta global intron

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CamkII-bPac(WT)-mCherry-minWPRE.sbd

   Plasmid

   #118278

   Purpose

   Expresses bPAC(WT)-mCherry under a minimal CamKII Promoter

   Depositor

   Dietmar Schmitz

   Article

   Bernal Sierra et al Nat Commun. 2018 Nov 5;9(1):4611. doi: 1

   Insert

   bPAC(WT)
   

   Use

   AAV

   Tags

   mCherry and myc

   Promoter

   CamKII

   Available Since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAc-His-hAR-2

   Plasmid

   #89091

   Purpose

   baculovirus transfer vector for histidine-tagged full-length human androgen receptor, starts Met-Ala-His6-Met-hAR

   Depositor

   Elizabeth Wilson

   Article

   Liao et al Biochemistry. 1999 Jul 27;38(30):9718-27

   Insert

   hAR (AR Human)
   

   Tags

   His

   Expression

   Mammalian

   Available Since

   April 19, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-TRE-DIO-GCaMP6s

   Plasmid

   #183809

   Purpose

   his plasmid is for use with neuronal cell-type selective activity tagging. The GCaMP6 expression requires both neural activity and Cre recombinase.

   Depositor

   William Wisden

   Article

   Yu et al Science. 2022 Jul;377(6601):63-72. doi:

   Insert

   AAV transgene TetO promoter-DIO/flex-GCaMP6
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Expression

   Mammalian

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-AAVS1-PGK-Puro-moxGFP

   Plasmid

   #227275

   Purpose

   AAVS1 targeting donor for the insertion of Puro and a medium strength PGK expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   AAVS1 Homology Arms flanking a 2A-Puro-PGK-moxGFP cassette (AAVS1 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGreenII 0229/ProRUBY:GOXL1-Citrine:NOSt

   Plasmid

   #175563

   Purpose

   pSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-1

   Depositor

   George Haughn

   Article

   Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

   Insert

   GOXL1
   

   Tags

   Citrine

   Expression

   Plant

   Promoter

   RUBY

   Available Since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-MCS-shCHD5 #1

   Plasmid

   #68876

   Purpose

   CHD5 shRNA expressed from Adeno-associated viral (AAV) vector

   Depositor

   Adrian Bracken

   Article

   Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

   Insert

   CHD5
   

   Use

   AAV and RNAi

   Expression

   Mammalian

   Promoter

   H1

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pAAV2_hSyn_NES-Caprola_on-mEGFP_WRPE-SV40

   Plasmid

   #194691

   Purpose

   hSyn1 driven expression of the consitutively active calcium recorder positive control Caprola_on fused to mEGFP for neuronal expression through AAV transduction

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_on-mEGFP
   

   Use

   AAV

   Tags

   mEGFP

   Promoter

   hSyn1

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-CaMKIIa-eArchT 3.0-EYFP

    Plasmid

    #35513

    Purpose

    Lentiviral expression of CaMKIIa-driven ArchT 3.0 for optical inhibition.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    eArchT 3.0-EYFP
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Enhanced trafficking signal and ER export signal …

    Promoter

    CaMKIIa

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-MCS-shCHD5 #2

    Plasmid

    #68877

    Purpose

    CHD5 shRNA expressed from Adeno-associated viral (AAV) vector

    Depositor

    Adrian Bracken

    Article

    Egan et al Dev Cell. 2013 Aug 12;26(3):223-36. doi:

    Insert

    CHD5
    

    Use

    AAV and RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pAAV-DIO-NS1

    Plasmid

    #175273

    Purpose

    AAV vector mediating Cre-depenent expression of NS1 gene of YFV-17D

    Depositor

    Wei Xu

    Article

    Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

    Insert

    Nonstructural protein 1 (NS1) of YFV-17D (POLY Yellow fever virus strain 17D)
    

    Use

    AAV

    Promoter

    Synapsin

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV_Efs_hSpCas9_NLS_FLAG-SV40

    Plasmid

    #97307

    Purpose

    AAV vector for encoding a human codon-optimized SpCas9 driven by EFs promoter

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    humanized S. pyogenes Cas9
    

    Use

    AAV and Mouse Targeting

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-ChRmine-eYFP-WPRE

    Plasmid

    #130992

    Purpose

    AAV vector to drive the expression of ChRmine-eYFP under the control of human synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    eYFP

    Promoter

    hSyn

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. HT109_pAAV_hSyn-DiO-SomQuasAr6a_EGFP

    Plasmid

    #190878

    Purpose

    Cre-on expression of soma-targeted QuasAr6a under an neuronal promoter

    Depositor

    Adam Cohen

    Article

    Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

    Insert

    somQuasAr6a_EGFP
    

    Use

    AAV

    Tags

    EGFP

    Promoter

    hSyn

    Available Since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CAG-ArchT-tdTomato

    Plasmid

    #29778

    Purpose

    AAV-mediated expression of ArchT-tdTomato for neuronal inhibition

    Depositor

    Edward Boyden

    Article

    Han et al Front Syst Neurosci. 2011;5:18. Epub 201

    Insert

    ArchT-tdTomato
    

    Use

    AAV; Adeno-associated virus

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40

    Plasmid

    #100850

    Purpose

    AAV expression of Cre recombinase-activated jRCaMP1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1

    Insert

    jRCaMP1b
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    March 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV ORANGE Gria1-HaloTag KI

    Plasmid

    #139655

    Purpose

    Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and HaloTag donor
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBrain-GFP-FKBP-TACC3-shTACC3

    Plasmid

    #59354

    Purpose

    Knocks down endogenous TACC3 and expresses RNAi-resistant human TACC3 tagged with GFP & FKBP in mammalian cells.

    Depositor

    Stephen Royle

    Article

    Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

    Insert

    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    

    Use

    RNAi

    Tags

    EGFP and FKBP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTFG005_Spike_Inducible_3xFlag

    Plasmid

    #191578

    Purpose

    Inducible expression of SARS-CoV-2 Spike protein with C-terminal 3x FLAG tag under TRE3G promoter and cloned into a 2nd generation lentiviral transfer plasmid (modified pLVX with Hygro resistance).

    Depositor

    Joshua Leonard

    Article

    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

    Insert

    SARS-CoV-2 Spike Protein (S Synthetic)
    

    Use

    Lentiviral

    Tags

    3x FLAG

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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