We narrowed to 17,816 results for: Met;

1. pOTTC351 - pAAV CaMKIIa hChR2(H134R)-iRFP

   Plasmid

   #47905

   Purpose

   An AAV vector that expresses channel rhodopsin 2 (H134R) (fused to iRFP) under the CaM Kinase IIa promoter.

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   hChR2(H134R)
   

   Use

   AAV

   Tags

   iRFP

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   CaMKIIa

   Available since

   Sept. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. mIFP12-FilaminA-C-14

   Plasmid

   #56253

   Purpose

   Localization: Actin/Focal Adhesions, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   FilaminA (FLNA Human)
   

   Use

   Tags

   mIFP12

   Expression

   Mammalian

   Mutation

   D2626H

   Promoter

   CMV

   Available since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. mIFP12-LaminA-C-18

   Plasmid

   #56256

   Purpose

   Localization: Nuclear Envelope, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   LaminA (LMNA Human)
   

   Use

   Tags

   mIFP12

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pAAV-CAG-NLS-DNMT3A-L-N-spC9-N-intein-hGH

   Plasmid

   #112212

   Purpose

   Targeted DNA methylation

   Depositor

   Xingxu Huang

   Article

   Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

   Insert

   N-terminus of dCas9 and DNMT3A (DNMT3A Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   H840A,N863A

   Promoter

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268

   Plasmid

   #69219

   Purpose

   Expresses wild type SpCas9 fused to Zif268 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   Zif268 (Egr1 Mouse)
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. JA412

   Plasmid

   #49935

   Purpose

   Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human KLF4 gene and demethylate CpGs adjacent to the binding site

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   Tet1CD KL-1 (KLF4 Human)
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-EF1α1.1-Chronos-GFP

   Plasmid

   #122098

   Purpose

   AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α (1.1 kb short version)

   Available since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-8

   Plasmid

   #129036

   Purpose

   targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA8 targeting human TSDR

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA8 (huTSDR)
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. SL357

   Plasmid

   #49936

   Purpose

   Expresses a TALE-TET1FL (full length TET1) fusion protein engineered to bind a site in the human KLF4 gene and demethylate CpGs adjacent to the binding site

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   Tet1FL KL-1 (KLF4 Human)
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pEAHISMRSA2676

    Plasmid

    #96970

    Purpose

    expression of methicillin-resistant Staphylococcus aureus orf 2676

    Depositor

    James Naismith, Huanting Liu

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    Sar2676
    

    Use

    Tags

    N-ter TEV protease cleavable 6His

    Expression

    Mutation

    none

    Promoter

    T7

    Available since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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