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pHR-flag-GFP-JAM-B

Plasmid

#205191

Purpose

This vector encodes of a synCAM (JAM-B) and GFP

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM JAM-B and GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2_mSTING_scrambled_gRNA_2

Plasmid

#196628

Purpose

Scrambled control 2 for STING knock-out in murine cells.

Depositor

Nicolas Manel

Article

Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

Insert

mSTING scrambled gRNA 2

Use

CRISPR and Lentiviral

Mutation

WT

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCVII-mChe

Plasmid

#162753

Purpose

Retroviral (MSCV) expression of mCherry fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

mCherry

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-CD4-sTC-M13-GFP-IRES-CaM-V5-sTN

Plasmid

#219782

Purpose

CaST-IRES for HEK cell expression

Depositor

Christina Kim

Article

Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

Insert

CD4-sTC-M13-GFP-IRES-CaM-V5-sTN

Use

AAV

Tags

HA and V5

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-CEBPA

Plasmid

#219393

Purpose

Express CEBPA in mammalian cells

Depositor

Yoichi Furukawa

Article

Nakagawa et al Commun Biol. 2024 Apr 29;7(1):510. doi:

Insert

CEBPA (CEBPA Human)

Tags

N terminal Myc tag

Expression

Mammalian

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a-hP53DD

Plasmid

#214080

Purpose

Mammalian expression of human P53DD

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

hP53DD (TP53 Human)

Expression

Mammalian

Promoter

EF1a

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-hsRPA32 (MSW#497)

Plasmid

#208068

Purpose

Expression of GFP-tagged hsRPA2 in human cells

Depositor

Marc Wold

Article

Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

Insert

RPA2 (RPA2 Human)

Tags

GFP

Expression

Mammalian

Mutation

T88I- please see depositor comments

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+N-eGFP_SAMD9L

Plasmid

#206173

Purpose

mammalian expression of WT SAMD9L

Depositor

Christopher Goodnow

Article

Russell et al Proc Natl Acad Sci U S A. 2021 Aug 24;11

Insert

SAMD9L (SAMD9L Human)

Tags

GFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-Wt-PTEN

Plasmid

#206982

Purpose

Lentiviral vector for expressing doxycyclin-inducible cDNA of wild type PTEN

Depositor

Ming Chen

Article

Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

Insert

PTEN (PTEN Human)

Use

Lentiviral

Expression

Mammalian

Promoter

tet-on promoter

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G4_Q118R_Std_BFP-to-EGFP_Dual_epegRNA_tevopreQ1

Plasmid

#187459

Purpose

Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 pegRNA and EBFP_To_EGFP epegRNA (tevopreq1) from two independent U6 promoters.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G4 Q118R pegRNA + EBFP to EGFP epegRNA (tevopreq1) (ATP1A1 Human)

Use

CRISPR; Prime editing

Expression

Mammalian

Promoter

Tandem U6 promoters

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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cmv-sfGFP-GB1-scAB-ubc-nls-pcp-12xSunTag(SRv1.2-2P)

Plasmid

#185802

Purpose

one plasmid encoding scFv GFP and pcp-10xSunTag components for SunRISER SRv1.2

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

cmv-sfGFP-GB1-scAB--ubc-nls-pcp-12xSunTag

Expression

Mammalian

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pH6HTC_IGF2BP1-Halo

Plasmid

#175300

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

IGF2BP1 (IGF2BP1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-TPD54

Plasmid

#172449

Purpose

Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry tag

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2020 Jan 6;219(1). pii: jcb

Insert

TPD54 (TPD52L2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCB301TOR-CRISPR

Plasmid

#164797

Purpose

For gene editing in oomycetes

Depositor

Miaoying Tian

Article

Gumtow et al Mol Plant Microbe Interact. 2018 Mar;31(

Inserts

PsNLS-hSpCas9
sgRNA expression cassette

Use

Oomycete expression

Promoter

HAM34 and RPL41

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pGI3EM20C

Plasmid

#135487

Purpose

Agrobacterium T-DNA with divergent Spun H2A/Bpr driving H2B-tdTomato and hph

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

hph SpunH2A/Bpr H2B-tdTomato

Tags

tdTomato

Expression

Bacterial and Yeast

Promoter

SpunH2A/Bpr (divergent)

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pGWB2-CYCD3;1

Plasmid

#140750

Purpose

Inducing plant cells to enter mitosis

Depositor

Bo Liu

Article

Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

Insert

Cyclin D3;1 (CYCD3;1 Mustard Weed)

Expression

Plant

Promoter

35S

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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p5xNF-kb_RE

Plasmid

#118050

Purpose

GBPart - Operator/DNA Response element - 5 copies of the NF-kb DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

5 copies of the NF-kb DNA binding motif

Use

Synthetic Biology; Domestication of dna parts for…

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-D169G

Plasmid

#133757

Purpose

Lentiviral expression of human TDP-43 D169G disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed aspartate 169 to glycine.

Promoter

CMV

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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ETV4_pet28a

Plasmid

#131645

Purpose

Bacterial expression of ETS gene ETV4

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV4 (ETV4 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA_RGG-GFP-RGG_withTEVsite

Plasmid

#124939

Purpose

Expresion of RGG_GFP-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

GFP

Expression

Mammalian

Mutation

TEV site between RGG and EGFP

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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