We narrowed to 55,051 results for: plasmid
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Plasmid#222828PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (EC1 promoter, bialaphos and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only
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pHEE-R-hyg5A
Plasmid#222829PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (RPS5A promoter, hygromycin and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-kan5A
Plasmid#222830PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (RPS5A promoter, kanamycin and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEE-R-bar5A
Plasmid#222831PurposeCRISPR/Cas9 for multi-gene knockout in Arabidopsis (RPS5A promoter, bialaphos and red fluorescent seed selection)DepositorTypeEmpty backboneUseCRISPRAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-SYN-mCherry-oPRE
Plasmid#218682PurposeAAV vector plasmid expressing mCherry under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.DepositorInsertmCherry
UseAAVExpressionMammalianAvailable SinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T11 Circuit 2 GG4-PhiC31B-Bxb1B-GG6
Plasmid#219694PurposeLevel 0 to construct history-dependent target T2BPDepositorInsertGG4-PhiC31B-Bxb1B-GG6
UseSynthetic BiologyAvailable SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-35aaNSP1SA11-NSP5(wt)
Plasmid#220823PurposeExpress the protein consisting of 35 amino acid of Rotavirus SA11 NSP1 protein and the full-length SA11 NSP5 proteinDepositorInsertRotavirus SA11 35aaNSP1-NSP5(wt)
UseOtherAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-35aaNSP1SA11-NSP5(io)
Plasmid#220824PurposeExpress the protein consisting of 35 amino acid of Rotavirus SA11 NSP1 protein and the full-length recoded NSP5 proteinDepositorInsertRotavirus SA11 35aaNSP1-NSP5(io)
UseOtherAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-NSP1delta103CSA11-GFP
Plasmid#220813PurposeExpress the Rotavirus SA11 delta103C NSP1 protein fused with the sfGFP (Pedelacq et al., 2006)DepositorInsertRotavirus SA11 NSP1delta103C-GFP
UseOtherMutationLacks C-terminal 103 aaAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-NSP3SA11-F2A-EGFP
Plasmid#220808PurposeExpress separately the Rotavirus SA11 NSP3 protein and the original enhanced GFP (Yang et al., 1996)DepositorInsertRotavirus SA11 NSP3-F2A-EGFP
UseOtherAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-rDPP9(S729A)
Plasmid#217327PurposePurification of catalytically-dead rat DPP9 from insect cellsDepositorInsertDPP9
TagsHis-TEVExpressionInsectMutationS729APromoterpolyhedrinAvailable SinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-C675S
Plasmid#216756PurposeInvestigation of the potential interaction of the FeoB Cys675 residue with ferrous ironDepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
Tags8x HisExpressionBacterialMutationAmino acid C675 is substituted with S.Available SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-C429S
Plasmid#216755PurposeInvestigation of the potential interaction of the FeoB Cys429 residue with ferrous ironDepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
Tags8x HisExpressionBacterialMutationAmino acid C429 is substituted with S.Available SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-D123N
Plasmid#216754PurposeProduction of a FeoB that is unable to bind GTP for a negative control in GTPases assaysDepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
Tags8x HisExpressionBacterialMutationAmino acid D123 is substituted with N.Available SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T12 GG2-ER-tagBFP-tUBQ10-GG3
Plasmid#219695PurposeLevel 0 to construct history-dependent targetDepositorInsertGG2-ER-tagBFP-tUBQ10-GG3
UseSynthetic BiologyAvailable SinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I75_tUBQ10_GG5-GG6
Plasmid#219714PurposeLevel 0 tUBQ10 terminator, GG5 and GG6 BsaI sites for golden gate assembly.DepositorInsertGG5-tUBQ10-GG6
UseSynthetic BiologyMutationWT terminator with added golden gate linkersAvailable SinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOCC102-MBP-eIF4G-SkA
Plasmid#219977PurposeeIF4G-SkA protein purificationDepositorInserteIF4G-SkA (TIF4631 Budding Yeast)
Tags6xHis-MBPExpressionInsectMutationeIF4A binding domain of S. kudriavzeviiAvailable SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOCC102-MBP-eIF4G-RAR
Plasmid#219973PurposeeIF4G-RAR protein purificationDepositorInserteIF4G-RAR (TIF4631 Budding Yeast)
Tags6xHis-MBPExpressionInsectMutationTruncation: amino acids 490 to 942Available SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T13 GG3-tRBCS-mCherry-NLS-GG4
Plasmid#219696PurposeLevel 0 to construct history-dependent targetDepositorInsertGG3-tRBCS-mCherry-NLS-GG4
UseSynthetic BiologyAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T14 GG0-tUBQ1-NLS-NeonGreen-GG1
Plasmid#219697PurposeLevel 0 to construct history-dependent targetDepositorInsertGG0-tUBQ1-NLS-NeonGreen-GG1
UseSynthetic BiologyAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only