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pGL4.23 D637G 2014 EBOV + Mucin-Like Domain

Plasmid

#86018

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Changed aspartic acid 637 to glycine

Promoter

CMV IE

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pMCB320-sgNC.ACOCA

Plasmid

#169836

Purpose

Expresses a negative control sgRNA

Depositor

Laura Attardi

Article

Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

Insert

safe harbor sgRNA

Use

CRISPR, Lentiviral, and Mouse Targeting

Expression

Mammalian

Promoter

mU6

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-sgZmat3.55.9/Cre

Plasmid

#167853

Purpose

Expresses Cre recombinase and an sgRNA targeting Zmat3

Depositor

Laura Attardi

Article

Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

Insert

sgRNA targeting Zmat3

Use

CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

Expression

Mammalian

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMCB320-sgZmat3.51.5

Plasmid

#169024

Purpose

Expresses an sgRNA targeting Zmat3

Depositor

Laura Attardi

Article

Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

Insert

sgRNA targeting Zmat3, mCherry, Puromycin resistance

Use

CRISPR, Lentiviral, and Mouse Targeting

Expression

Mammalian

Promoter

mU6

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMCB320-sgZmat3.55.9

Plasmid

#169026

Purpose

Expresses an sgRNA targeting Zmat3

Depositor

Laura Attardi

Article

Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

Insert

sgRNA targeting Zmat3, mCherry, Puromycin resistance

Use

CRISPR, Lentiviral, and Mouse Targeting

Expression

Mammalian

Promoter

mU6

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-NSD3-short del(384-645)

Plasmid

#72549

Purpose

expresses 3*FLAG tagged human NSD3-short with 384-645 aa deletion

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

NSD3-short (NSD3 Human)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

384-645 aa deletion

Promoter

LTR

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(WT)--IRES-hyg

Plasmid

#128667

Purpose

mammalian expression of Flag-Polß(WT) protein with hygromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Wild type

Promoter

CMV

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-YAP-S381A

Plasmid

#33090

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 )

Tags

Flag

Expression

Mammalian

Mutation

S381A

Promoter

CMV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
MSCV-NSD3-short del(1-100)

Plasmid

#72547

Purpose

expresses 3*FLAG tagged human NSD3-short with 1-100 aa deletion

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

NSD3-short (NSD3 Human)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

1-100 aa deletion

Promoter

LTR

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (-) hYAP 1-2beta

Plasmid

#124144

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (-) hYAP 1-1gamma

Plasmid

#124141

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLPC-NMYC-hTRF1deltaBLM

Plasmid

#64165

Purpose

Retroviral vector expressing human TRF1 delta aa317-374 with N-terminal MYC tag

Depositor

Titia de Lange

Article

Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

deleted amino acids aa317-374 of human TRF1

Promoter

CMV

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only
pAAV.CAG-FLEX.SF-iGluSnFR.S72A

Plasmid

#106188

Purpose

Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.S72A

Use

AAV

Mutation

GltI: S72A

Promoter

CAG

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shGDF11 #1

Plasmid

#83083

Purpose

Lentiviral shRNA vector for inducible knockdown of human GDF11 (cross reacts with mouse Gdf11)

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shGDF11 (GDF11, Gdf11 Human, Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pBABEhygro-Flag-ZO-2-delta-N

Plasmid

#27476

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Deletion of coding nucleotides from 1-351. This r…

Available Since

March 31, 2011

Availability

Academic Institutions and Nonprofits only
pBABE (hygro) mEOS2 hYAP 1-2delta

Plasmid

#124162

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

mEOS2-YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.23 A82V/T230A 2014 EBOV Delta-Mucin-Like-Domain

Plasmid

#86025

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Lacks mucin-like domain; Changed alanine 82 to va…

Promoter

CMV IE

Available Since

July 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.23 A82V/T230A 2014 EBOV + Mucin-Like Domain

Plasmid

#86019

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Jeremy Luban, Pardis Sabeti

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Expression

Mammalian

Mutation

Changed alanine 82 to valine and threonine 230 to…

Promoter

CMV IE

Available Since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pMIR-Report-RASA1-3'UTR

Plasmid

#62575

Purpose

Translational Luciferase Reporter containing a 926 bp fragment of the RASA1 3'UTR.

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

RASA1 (RASA1 Human)

Use

Luciferase

Tags

None

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pRCIscript-crhr1_TAL1B

Plasmid

#44235

Depositor

Karl Clark, Stephen Ekker

Article

Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

Insert

crhr1 pTAL scaffold 1B (crhr1 Zebrafish)

Use

Mrna transcription vector

Promoter

T3

Available Since

April 10, 2013

Availability

Academic Institutions and Nonprofits only

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