We narrowed to 9,895 results for: EPO

1. AAV.CBA.eGFP.2A.P301L-Tau

   Plasmid

   #140425

   Purpose

   AAV.CBA.eGFP.2A.P301L-Tau propagation reporter P301L-2N4R-hTau

   Depositor

   Bradley Hyman

   Article

   Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi:

   Insert

   eGFP-2A-Tau(P301L) (MAPT Synthetic, Human)
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mutation

   changed Proline 301 to Leucine

   Promoter

   CBA

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXJ385-pAAV-PB3’IR-U6-gRNA-EF1a-mScarlet-KASH-PB5’IR

   Plasmid

   #224435

   Purpose

   AAV piggybac transposon vector, with mScarlet-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

   Depositor

   Xin Jin

   Article

   Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

   Inserts

   mScarlet-KASH
   U6-gRNA
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXJ220-pAAV-PB3'IR-U6-gRNA-EF1a-GFP-KASH-PB5'IR

   Plasmid

   #213481

   Purpose

   AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

   Depositor

   Xin Jin

   Article

   Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

   Inserts

   GFP-KASH
   U6-gRNA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXs-IP-mVenus-TOSI

   Plasmid

   #172491

   Purpose

   mVenus-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.

   Depositor

   David Scadden

   Article

   Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

   Insert

   Pdcd4 (Pdcd4 Mouse)
   

   Use

   Retroviral

   Tags

   mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   MLV LTR

   Available Since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

   Plasmid

   #99278

   Purpose

   Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance

   Depositor

   Jordan Miller

   Article

   Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

   Inserts

   H2B-mVenus-IRES-mCherry
   Puro
   

   Use

   Lentiviral

   Tags

   H2B, IRES, PGK, mCherry, and mVenus

   Expression

   Mutation

   Promoter

   EF1 alpha-human and PGK-mouse

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W

   Plasmid

   #67980

   Purpose

   Cas9 activity reporter with BFP and GFP.

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcs2-ATF4uORF1-2-GFP-IRES-mCherry

   Plasmid

   #226105

   Purpose

   ATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   atf4 (ATF4 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contains uORF1-2 of ATF4, whose stability can ser…

   Promoter

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL4-AARE-luc2P-Hygro

   Plasmid

   #101787

   Purpose

   Luferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)

   Depositor

   Seiichi Oyadomari

   Article

   Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

   Insert

   3xAARE
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   minimal TATA-box promoter with low basal activity

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRRL-SV40-Puro-CMV-mCherry-EGFP-LC3B

   Plasmid

   #223712

   Purpose

   Expresses autophagy flux LC3B fluorescence reporter

   Depositor

   Benjamin Wolozin

   Article

   Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

   Insert

   LC3B (MAP1LC3B Synthetic, Human)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   mCherry::EGFP::LC3B

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. PCR4-Shh::lacZ-H11

    Plasmid

    #139098

    Purpose

    enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Shh minimal promoter)

    Depositor

    Len Pennacchio

    Article

    Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Shh

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti_EF1a_Flag_TP53_WT

    Plasmid

    #183112

    Purpose

    Expresses flag-tagged p53 in mammalian cells

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    TP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Promoter

    EF1a

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. PCR4-Hsp68::lacZ-H11

    Plasmid

    #139099

    Purpose

    enSERT LacZ reporter vector for site-specific integration into the H11 locus (contains Hsp68 minimal promoter)

    Depositor

    Len Pennacchio

    Article

    Kvon et al Cell. 2020 Mar 6. pii: S0092-8674(20)302

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Hsp68

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSMALB-ATF4.5

    Plasmid

    #155032

    Purpose

    Fluorescent reporter for ATF4 translation

    Depositor

    Peter van Galen, John Dick

    Article

    van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

    Inserts

    ATF4 5' UTR (Atf4 Mouse)
    ATF4-eGFP fusion ORF
    TagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiCMV Puro DEST p38KTRmCerulean3

    Plasmid

    #59155

    Purpose

    Lentiviral vector to express p38 KTR mCerulean3 under CMV promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
    

    Use

    Lentiviral

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. TDP-REGv2(Gaussia Luciferase)#5 in pTwist-CMV

    Plasmid

    #216164

    Purpose

    Expression of secreted Gaussia Luciferase specifically in cells with TDP-43 loss of function. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    Gaussia princeps luciferase (with double mutation to improve stability)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXJ218-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-mtagBFP2-KASH-PB5'IR

    Plasmid

    #213480

    Purpose

    AAV piggybac transposon vector, with mtagBFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

    Depositor

    Xin Jin

    Article

    Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

    Inserts

    mtagBFP-KASH
    U6-gRNA-directcapture
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-THRA-RFP-EGFP

    Plasmid

    #228537

    Purpose

    Bichromatic fluorescent reporter of THRA isoform 1 and isoform 2 splicing in mammalian cells

    Depositor

    Markus Schuelke

    Article

    Graceffo et al Int J Mol Sci. 2024 Dec 17;25(24):13512.

    Insert

    Thyroid Hormone Receptor Alpha (THRA Human)
    

    Use

    Tags

    eGFP and tagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-hygro-STING HAQ+V155M

    Plasmid

    #102601

    Purpose

    Express STING V55M HAQ for IFN-beta Luciferase reporter assay.

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    V155M R71H-G230A-R293Q

    Promoter

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-N-Flag-ASC1

    Plasmid

    #75134

    Purpose

    Expresses N-terminal Flag-tagged ASC1 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    PYD and CARD domain containing (Pycard Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F23S (please see depositor comments below)

    Promoter

    CMV

    Available Since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSMALB-ATF4.12

    Plasmid

    #155033

    Purpose

    Fluorescent reporter for ATF4 translation, Negative Control

    Depositor

    Peter van Galen, John Dick

    Article

    van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

    Inserts

    ATF4 5' UTR (Atf4 Mouse)
    ATF4-eGFP fusion ORF
    TagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    uORF1 start codon mutated

    Promoter

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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