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We narrowed to 24,909 results for: promoter

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Showing: 9241 - 9260 of 24909 results

  1. 3` CC: Hygro – GFP-N – loxP – mScarlet

    Plasmid
    #219564
    Purpose
    3` circularization cassette.To induce Cre-mediated circularization or inversion of a genomic region with concomitant GFP reconstitution and mScarlet expression from the chromosome
    Depositor
    Andrea Ventura
    Article
    Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586
    Insert
    3` CC: Hygro – GFP-N – loxP – mScarlet
    Use
    Unspecified
    Promoter
    EF1-a
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    219564-plasmid-map-sequence-id-438335

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  2. CMV-minidCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA

    Plasmid
    #171385
    Purpose
    Expression vector for encoding a human codon-optimized minidCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.
    Depositor
    Hui Yang
    Article
    Xu et al Nat Methods. 2021 May;18(5):499-506. doi
    Insert
    humanized minidCas13X.1-RESCUE-S
    Expression
    Mammalian
    Promoter
    CMV, U6
    Available Since
    July 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    171385-plasmid-map-sequence-id-337353

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  4. lenti-sgRNA puro

    Plasmid
    #104990
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and puromycin resistance from an EF-1a promoter.
    Depositor
    Brett Stringer
    Article
    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104990-plasmid-map-sequence-id-443800

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  5. lenti-sgRNA neo

    Plasmid
    #104992
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and G418 (neomycin) resistance from an EF-1a promoter.
    Depositor
    Brett Stringer
    Article
    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104992-plasmid-map-sequence-id-213507

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  7. gTBEv5

    Plasmid
    #220621
    Purpose
    vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv5) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Commun. 2024 Jun 8;15(1):4897. doi:
    Insert
    nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)
    Expression
    Mammalian
    Mutation
    hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…
    Promoter
    hU6, EF1a
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220621-plasmid-map-sequence-id-436953

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  9. pCMV-T7-ABE8e-SpRY-HF1-P2A-EGFP (LM434)

    Plasmid
    #197507
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HF1(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpRY-HF1-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpRY-HF1(D10A/A61R/N…
    Promoter
    CMV and T7
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197507-plasmid-map-sequence-id-386698

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  10. dCas12Max-ABE

    Plasmid
    #195335
    Purpose
    vector for encoding a human codon-optimized dCas12Max-ABE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.
    Insert
    human codon-optimized dCas12Max-ABE
    Expression
    Mammalian
    Mutation
    N243R, E336R, D1049A
    Promoter
    CBh, CMV, hU6
    Available Since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    195335-plasmid-map-sequence-id-383124

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  12. pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2]

    Plasmid
    #84445
    Purpose
    AAV-mediated expression of Jaws-KGC-GFP-ER2 under the CAG promoter, in floxed/reversed (Cre-dependent) manner.
    Depositor
    Edward Boyden
    Article
    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi
    Has Service
    AAV Retrograde, AAV1, and AAV5
    Insert
    Jaws-KGC-GFP-ER2
    Use
    AAV
    Tags
    ER2, GFP, and KGC
    Expression
    Mammalian
    Mutation
    K200R W214F
    Promoter
    CAG
    Available Since
    April 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    84445-plasmid-map-sequence-id-177041

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  13. DBDmut-CA-RIT-NFAT1

    Plasmid
    #63669
    Purpose
    Constitutively active NFAT1 and also unable to interact with AP-1 with four point mutations in the DNA binding loop that abolish DNA binding
    Depositor
    Anjana Rao
    Article
    Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15
    Insert
    NFAT1 (Nfatc2 Mouse)
    Use
    Retroviral
    Tags
    3x HA
    Expression
    Mammalian
    Mutation
    CA: PASSGSSASF mutated to PAAAGAAAAF, SPRTSPIMSP…
    Available Since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196084
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196084-plasmid-map-sequence-id-388993

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  15. pBFC0687

    Plasmid
    #186692
    Purpose
    ShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_2xBsaI_NT gRNA, GmR+sfGFP cargo
    Depositor
    Jennifer Doudna
    Article
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsB
    tnsC
    tniQ
    Cas12k
    Sh_2xBsaI_NT (Guide Stuffer) gRNA
    Tn7 transposon
    GmR+sfGFP ShDART Cargo
    Expression
    Bacterial
    Promoter
    PLac
    Available Since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186692-plasmid-map-sequence-id-371384

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  17. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

    Plasmid
    #73796
    Purpose
    pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA
    Depositor
    Ronald Davis
    Article
    Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1
    Inserts
    dCas9-Mxi1
    Tet Repressor
    Structural gRNA for S pyogenes
    Use
    CRISPR
    Tags
    Mxi1 and NLS
    Expression
    Yeast
    Mutation
    D10A, H840A
    Promoter
    pGPM1, pRPR1(TetO), and pTef1
    Available Since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    73796-plasmid-map-sequence-id-237125

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  18. pYLCRISPR/Cas9P35S-N

    Plasmid
    #66191
    Purpose
    expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), Neo selection
    Depositor
    Yao-Guang Liu
    Article
    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(
    Insert
    Cas9
    Expression
    Plant
    Mutation
    plant-codon optimized with higher GC contents (62…
    Promoter
    cauliflower mosaic virus 35S promoter (P35S)
    Available Since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    66191-plasmid-map-sequence-id-404047

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  20. pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)

    Plasmid
    #197502
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…
    Promoter
    CMV and T7
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197502-plasmid-map-sequence-id-386612

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Showing: 9241 - 9260 of 24909 results