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pLB62_PB_pGK-H2B-mIFP-T2A-rTetR-humanKRAB-zeo

Plasmid

#179439

Purpose

Expresses rTetR-KRAB(human) for gene silencing, integration by Piggybac

Depositor

Lacra Bintu

Article

Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

Insert

H2B-mIFP-T2A-rTetR-KRAB

Expression

Mammalian

Promoter

pGK

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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DNM2 delta PRD

Plasmid

#168275

Purpose

Express PRD-deleted DNM2 mutant (aa 1-742) in pEGFP-N1 vector.

Depositor

Cheng-han Yu

Article

Cao et al Commun Biol. 2020 Mar 13;3(1):117. doi:

Insert

DNM2 (DNM2 Human)

Tags

GFP

Expression

Mammalian

Mutation

aa 1-742

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSF11-wmiRFP2-T2A-HO1

Plasmid

#179485

Purpose

Expresses miRFP2 and HO1in worm cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

wmIRFP2-T2A-HO1

Expression

Mammalian

Mutation

NO

Promoter

tag-168 promoter

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE1-gRNA#2

Plasmid

#107727

Purpose

Knock out of EB1 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 gRNA #1 (targets Exon 1) (MAPRE1 Human)

Expression

Mammalian

Promoter

U6

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pMT-Pav-GFP

Plasmid

#24286

Depositor

Ron Vale

Article

Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

Insert

Pavarotti (pav Fly)

Tags

GFP

Expression

Insect

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
pBabe EGFR Del2(T790M)

Plasmid

#32075

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

Delete S752-I759, T790M

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
sgRNA BRCA1 E638K

Plasmid

#139327

Purpose

Plasmid expressing a sgRNA to introduce BRCA1 E638K using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA1 E638K using base editing

Expression

Mammalian

Promoter

U6

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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pBabe EGFR Del4(T790M)

Plasmid

#32074

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

Delete L747-P753, insert S, T790M

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pCIneoLuc-PP1A

Plasmid

#128348

Purpose

Vector for LUMIER assay

Depositor

Yutaka Hata

Article

Kawano et al J Biochem. 2015 May 14. pii: mvv051.

Insert

PP1A (PPP1CA Human)

Tags

Luciferase

Expression

Mammalian

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pEX-YFP-hLC3ΔG

Plasmid

#24990

Depositor

Isei Tanida

Article

Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

Insert

a mutant human MAP1-LC3B fused with EYFP (MAP1LC3B Human)

Tags

EYFP

Expression

Mammalian

Mutation

deleted Gly at 121

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only