We narrowed to 9,400 results for: BLI

1. pMinDis.X513-iNicSnFR3b-(V7)-ER

   Plasmid

   #125123

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

   Depositor

   Henry Lester

   Article

   Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

   Insert

   iNicSnFR3a-(V7)-ER
   

   Tags

   Fusion protein -Endoplasmic retention motif

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. KL001: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-VPR

   Plasmid

   #121836

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS/VPR scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. KL101: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-KRAB

   Plasmid

   #121837

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS/KRAB scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. KL202: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-LSD1

   Plasmid

   #121838

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS/LSD1 scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. KL401: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-Dnmt3a3L

   Plasmid

   #121840

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS/Dnmt3a-3L scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHHM.X513-iNicSnFR3a-(CC93)

   Plasmid

   #124880

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

   Depositor

   Henry Lester

   Article

   Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

   Insert

   iNicSnFR3a-(CC93)
   

   Tags

   HA Tag and His Tag

   Expression

   Bacterial

   Mutation

   NA

   Promoter

   T7

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHHM.X513-iNicSnFR2-(CC90)

   Plasmid

   #124836

   Purpose

   Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

   Depositor

   Henry Lester

   Article

   Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

   Insert

   iNicSnFR2-(CC90)
   

   Tags

   HA tags and His Tag

   Expression

   Bacterial

   Mutation

   NA

   Promoter

   T7

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.2 mir-1-1 reporter mir16-1.3

   Plasmid

   #46682

   Purpose

   Expresses a mutant version of the wild-type miR-16-1 in mammalian cells

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   mir16-1.3 (MIR16-1 Human, Synthetic)
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   see sequence and publication

   Promoter

   CMV

   Available Since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.2 mir-1-1 reporter mir16-1.1

   Plasmid

   #46680

   Purpose

   Expresses a mutant version of the wild-type miR-16-1 in mammalian cells

   Depositor

   David Bartel

   Article

   Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

   Insert

   mir16-1.1 (MIR16-1 Human, Synthetic)
   

   Use

   RNAi

   Tags

   V5 and hsa-mir-1-1

   Expression

   Mammalian

   Mutation

   see sequence and publication

   Promoter

   CMV

   Available Since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. KHBD00165

    Plasmid

    #34318

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG3411 (bs Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

11. LentiGuide-Puro-P2A-EGFP_mRFPstuf

    Plasmid

    #137730

    Purpose

    Expresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNA

    Depositor

    Fredrik Wermeling

    Article

    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, Mouse Targeting, and Syntheti…

    Expression

    Bacterial and Mammalian

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCIBN-EYFP-TetR

    Plasmid

    #103798

    Purpose

    BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-EYFP-TetR (CIB1, tetR E. coli, Mustard Weed)
    

    Expression

    Mammalian

    Mutation

    TetR: A4G (M2V)

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCIBN-EYFP-hPMLIII

    Plasmid

    #103808

    Purpose

    BLInCR 'Localizer' construct that marks the PML nuclear bodies and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-EYFP-hPMLIII (CIB1, PML Human, Mustard Weed)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. ABEmax-Puro V3

    Plasmid

    #226956

    Purpose

    ABEmax plasmid enabling A:T to G:C base editing using a single plasmid. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-MYO10-BioID

    Plasmid

    #194858

    Purpose

    Express MYO10 tagged with eGFP (Nter) and BioID (Cter) in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

    Insert

    MYO10 (MYO10 Human)
    

    Tags

    BioID and EGFP

    Expression

    Mammalian

    Mutation

    MyoX contains a Q680R variant compared to the NCB…

    Promoter

    CMV

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT2-hUbC-dCas9-KRAB-T2a-Puro

    Plasmid

    #225999

    Purpose

    To establish stable dCas9-KRAB knock-in line

    Depositor

    Miguel Ramalho-Santos

    Article

    Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

    Insert

    humanized dCas9-KRAB T2A Puro
    

    Use

    Sleeping beauty transposon

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21a-C2m2-SNAP

    Plasmid

    #208773

    Purpose

    To express C2m2-SNAP in bacteria

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2m2-SNAP (Mfge8 Mouse)
    

    Tags

    SNAP-tag

    Expression

    Bacterial

    Mutation

    Mutated 24 and 45 lysine to asparagine in C2 doma…

    Promoter

    T7 promoter

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21a-C2m2-mKate

    Plasmid

    #208765

    Purpose

    To express C2m2-mKate in bacteria

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2m2-mKate (Mfge8 Mouse)
    

    Tags

    mKate

    Expression

    Bacterial

    Mutation

    Mutated 24 and 45 lysine to asparagine in C2 doma…

    Promoter

    T7 promoter

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCIBN-tagBFP-hTRF2

    Plasmid

    #103803

    Purpose

    BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-tagBFP-hTRF2 (CIB1, TERF2 Human, Mustard Weed)
    

    Expression

    Mammalian

    Mutation

    hTRF2: deletion of amino acids 1-42 and 476 compa…

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK5 FLAG DEPTOR (13×S/T->A)

    Plasmid

    #21702

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

    Insert

    DEPTOR 13xA (DEPTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Nonphosphorylatable mutant. 13xA. Also has N204S …

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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