We narrowed to 17,594 results for: cript

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pLenti-CaMaKIIalpha-mKO2

Plasmid

#86156

Purpose

Lentiviral fluorescent reporter using the neuronal cell type-specific promoter of the CaM Kinase II alpha gene.

Depositor

James Ellis

Article

Rodrigues et al Cell Rep. 2016 Oct 11;17(3):720-734. doi

Insert

mKO2

Use

Lentiviral

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-TTF-1-HDD

Plasmid

#74991

Purpose

Retroviral vector holding human TTF-1 (NKX2-1) with the homeodomain deleted

Depositor

David Mu

Article

Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

Insert

TTF-1 HDD mutant (NKX2-1 Human)

Use

Retroviral

Tags

None

Expression

Mammalian

Mutation

HDD = homeodomain deleted

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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1130 pBabe puroL HA FKHR H215R AAA

Plasmid

#9035

Depositor

William Sellers

Article

Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

Insert

FKHR H215R AAA (FOXO1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

H215R T24A S256A S319A

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pAAV-DRD1-NNES-eLOV-TEVcs-Flag-Gal4-V5

Plasmid

#125227

Purpose

Expresses SPARK DRD1 transmembrane component in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

DRD1-NNES-eLOV-TEVcs-Flag-Gal4-V5

Use

AAV

Tags

Flag, V5

Expression

Mammalian

Promoter

CMV

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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-111 hTF m1

Plasmid

#15448

Depositor

Nigel Mackman

Article

Cui et al J Biol Chem. 1996 Feb 2. 271(5):2731-9.

Insert

Tissue factor promoter (F3 Human)

Use

Luciferase

Expression

Mammalian

Available Since

July 27, 2007

Availability

Academic Institutions and Nonprofits only
Pmyo3::tag:sl1(G16C)

Plasmid

#79003

Purpose

Expression of exogenous SL1 in C. elegans muscle

Depositor

Xiao Liu, Ge Zhan

Article

Ma et al Nucleic Acids Res. 2016 Aug 23. pii: gkw

Insert

sls-1.2 (sls-1.2 Nematode)

Tags

illumina-adapter

Expression

Worm

Mutation

G16C

Promoter

myo-3

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJZC625

Plasmid

#62321

Purpose

sgRNA with 1x MS2, Pol II promoter with ribozyme-gRNA-ribozyme design for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA +1x MS2, Pol II promoter with ribozyme cleavage

Expression

Yeast

Promoter

pAdh

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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MAC_N_IRF9

Plasmid

#187738

Purpose

MAC-tagged gene expression of human IRF9

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

IRF9 (IRF9 Human)

Expression

Mammalian

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_Myt1

Plasmid

#64845

Purpose

A lentiviral vector for the expression of mouse Myt1 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Myt1 (Myt1 Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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pIhh742bp-Luc

Plasmid

#53605

Purpose

Reporter construct containing a 742 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assays

Depositor

Xiangli Yang

Article

Wang et al Development. 2009 Dec;136(24):4143-53. d

Insert

Ihh promoter

Use

Luciferase; Reporter

Available Since

June 30, 2014

Availability

Academic Institutions and Nonprofits only
p5A0-T

Plasmid

#92016

Purpose

Expresses TALE targeted to lac operator without TEV cut sites, anhydrotetracycline inducible TEV protease

Depositor

Brian Pfleger

Article

Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

Inserts

TALE targeting lac operator
Tobacco Etch Virus Protease

Use

TALEN

Tags

5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

Mutation

S219V

Promoter

J23102 Promoter (from Anderson promoter library) …

Available Since

Jan. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCFL-mPML-2CAMut-iB

Plasmid

#140286

Purpose

Expression of mouse PML Ca mutant

Depositor

Yusuke Miyanari

Article

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

PML (Pml Mouse)

Expression

Mammalian

Mutation

CA mutant (RING finger mutant)

Promoter

CAG

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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459 pME-etv2

Plasmid

#49002

Purpose

full length coding sequence for zebrafish etv2 gene in gateway middle entry vector

Depositor

Nathan Lawson

Article

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ets variant gene 2 (etsrp Zebrafish)

Use

Gateway middle entry

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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GST-FOXO3a 1-506aa

Plasmid

#10826

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

GST-FOXO3a 1-506aa (FOXO3 Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pT7-SpCas9_sgRNA-site2 (RTW448)

Plasmid

#160137

Purpose

T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)

Use

In vitro transcription of sgrna from t7 promoter

Promoter

T7

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-2x

Plasmid

#172282

Purpose

Expressing EGFP mRNA fused with 2 tandem repeats of a 50-base sequence

Depositor

Antony K Chen

Article

Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

Insert

EGFP-N1-2x

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-4x

Plasmid

#172283

Purpose

Expressing EGFP mRNA fused with 4 tandem repeats of a 50-base sequence

Depositor

Antony K Chen

Article

Chen et al Sci Rep. 2017 May 8;7(1):1550. doi: 10.1

Insert

EGFP-N1-4x

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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GST-FOXO3a TM 1-506aa

Plasmid

#8351

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

FOXO3a 1-506aa (FOXO3 Human)

Tags

GST

Expression

Bacterial

Mutation

T32A, S253A, S315A

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

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miR-34aM3

Plasmid

#50829

Purpose

luciferase reporter for human miR-34a

Depositor

Yun Bai, Xueqing Xu

Article

Li et al BMC Mol Biol. 2012 Jan 31;13:4.

Insert

miR-34A promoter M3 (MIR34A Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

third NF-kB binding site mutated (-149)

Promoter

miR34a

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only
miR-34aM1

Plasmid

#50827

Purpose

luciferase reporter for human miR-34a

Depositor

Yun Bai, Xueqing Xu

Article

Li et al BMC Mol Biol. 2012 Jan 31;13:4.

Insert

miR-34A promoter M1 (MIR34A Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

first NF-kB binding site mutated, (-539)

Promoter

miR34a

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only