162,408 results

1. pET21a-LysRS-His

   Plasmid

   #124114

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Lysine-tRNA ligase (lysU Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-C1-AR

   Plasmid

   #28235

   Purpose

   Mammalian expression of Androgen receptor fused to EGFP

   Depositor

   Michael Mancini

   Article

   Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

   Insert

   Androgen receptor (AR Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMesh1*

   Plasmid

   #175591

   Purpose

   Lac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.

   Depositor

   Greg Bokinsky

   Article

   Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

   Insert

   Mesh1 (Mesh1 Fly)
   

   Use

   Tags

   Fused with a flexible GS linker to cerulean

   Expression

   Bacterial

   Mutation

   Codon optimized for translation in E.coli. AGGAGG…

   Promoter

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRRL-mU6-mScarletGO-P2A-Blas-PGK-Neo

   Plasmid

   #136904

   Purpose

   Lentivirus for base editing activatable mScarletI expression in mammalian cells. All-in-one vector with sgmScarletGO.

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   mScarletGo-p2a-Blas
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   ATG>ACG

   Promoter

   SFFV

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-CAG-dCasRx-RBM25-IRES-BleoR

   Plasmid

   #221002

   Purpose

   Stable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.

   Depositor

   Benjamin Blencowe, Mikko Taipale

   Article

   Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

   Insert

   dCasRx-RBM25-IRES-BleoR
   

   Use

   CRISPR

   Tags

   NLS and NLS-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5-EGFP-Tau AP

   Plasmid

   #46905

   Purpose

   expresses EGFP tagged Tau AP in mammalian cells

   Depositor

   Karen Ashe

   Article

   Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

   Insert

   Tau AP (MAPT Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   all 14 S/P or T/P amino acid residues (T111, T153…

   Promoter

   CMV

   Available since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST-SorLA-v1

   Plasmid

   #154892

   Purpose

   Expresses SorLA fused to Venus fragment 1 (v1) for use in bimolecular fluorescence complementation (BiFC) assays

   Depositor

   Johanna Ivaska

   Article

   Al-Akhrass et al Oncogene. 2021 Feb;40(7):1300-1317. doi:

   Insert

   SORL1 (SORL1 Human)
   

   Use

   Tags

   Venus fragment 1 (v1)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBMN(CMV-copGFP-Luc2-Puro)

   Plasmid

   #80389

   Purpose

   To make lentiviral vector expressing copGFP, firefly Luciferase and puromycin selection marker

   Depositor

   Magnus Essand

   Article

   Jin et al EMBO Mol Med. 2016 May 6. pii: e20150586

   Insert

   copGFP-T2A-Luc2-F2A-Puromycin
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiV2-hU6-evopreQ1

   Plasmid

   #210189

   Purpose

   pegRNA/ngRNA library cloning

   Depositor

   Yin Shen

   Article

   Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH-CMV

    Plasmid

    #72265

    Purpose

    Express gene of interest under CMV promoter

    Depositor

    Kazuhiro Oka

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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