We narrowed to 12,067 results for: 110

Showing: 9321 - 9340 of 12067 results

Results per page:

Sort results by:

pC3.1_CAG_oROS-Gr

Plasmid

#216113

Purpose

Expresses the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.

Depositor

Andre Berndt

Article

Lee et al bioRxiv [Preprint]. 2024 Feb 4:2024.01.3

Insert

CAG_oROS-Gr

Expression

Mammalian

Promoter

CAG

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
GAL FLAG103QDProGFPp303

Plasmid

#15587

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 103Q Delta Pro (HTT Human)

Tags

FLAG and GFP

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pETM33_SIN3A_PAH

Plasmid

#178486

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

SIN3A_PAH (SIN3A Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW57.1-4EBP1_4xAla

Plasmid

#38240

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

4EBP1 (Eif4ebp1 hybrid gene of rat and human, Rat)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Mutation

T37A, T46A, S65A, T70A (dominant negative)

Promoter

Tet ON

Available Since

Nov. 15, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
dSaCas9-VPR

Plasmid

#205251

Purpose

In vitro transcription plasmid for production of dSaCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #68495

Depositor

Rasmus Bak

Article

Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

Insert

dSaCas9-VPR

Use

CRISPR

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
SiRES COR1C-GFP

Plasmid

#195154

Purpose

image COR1C

Depositor

Gia Voeltz

Article

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

CORO1C (CORO1C Human)

Expression

Mammalian

Mutation

mutations to make si RNA resistant

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
lentiTet-P2A-mCherry BlastR

Plasmid

#186743

Purpose

Dox inducible P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

Depositor

Richard Sherwood

Article

Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

Insert

P2A-mcherry

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn1-FLEX-CybGam-WPRE

Plasmid

#207662

Purpose

Control for CybSEP2

Depositor

Sung Han

Article

Kim et al bioRxiv. 2023 Jan 20:2023.01.19.524797.

Insert

Gamillus

Use

AAV

Mutation

Histidine 86 and 159 to alanines, inserted gamill…

Promoter

hSynapsin

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Cas9 MDC123

Plasmid

#59184

Purpose

2x35S promoting Glycing Max codon optimized Cas9 with bar plant selection

Depositor

Robert Stupar

Article

Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

Insert

Glycine Max codon optimized Cas9

Use

CRISPR

Expression

Plant

Promoter

2X35S

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBK1302-AAV-EFSNC-dCjCas9-KRAB

Plasmid

#223147

Purpose

Expression of KRAB with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD_B2520

Plasmid

#91077

Purpose

Module B, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Esp3I ccdb cassette for single gRNA spacer cloning

Use

CRISPR

Promoter

OsU6

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Stat3-C Flag pRc/CMV

Plasmid

#8722

Depositor

Jim Darnell

Article

Bromberg et al Cell 1999 Aug 6;98(3):295-303.

Insert

Stat3-C (Stat3 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

A662C and N664C. Residues changed to Cys renders…

Available Since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)/AUG-nLuc-3XFLAG

Plasmid

#127299

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDIRECT_22A

Plasmid

#91133

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:AtCas9 + AtU6:gRNA

Use

CRISPR

Expression

Plant

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMMP-puro-Flag-HA-NR6A1

Plasmid

#46833

Depositor

Phil Sharp

Article

Gurtan et al Genes Dev. 2013 Apr 15;27(8):941-54. doi

Insert

NR6A1 (Nr6a1 Mouse)

Use

Retroviral

Tags

Flag-HA, plus 8-amino acid residue linker

Expression

Mammalian

Mutation

None

Available Since

Nov. 25, 2013

Availability

Academic Institutions and Nonprofits only
qs5

Plasmid

#24498

Depositor

Bruce Conklin

Article

Conklin et al Mol Pharmacol. 1996 Oct . 50(4):885-90.

Insert

Gq alpha (Gnaq Mouse)

Tags

HA (see comments)

Expression

Mammalian

Mutation

Replacement of the five carboxyl-terminal amino a…

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
G10 Cas9 MDC123

Plasmid

#59187

Purpose

G10 promoting Glycing Max codon optimized Cas9 (N and C terminus NLS) with bar plant selection

Depositor

Robert Stupar

Article

Michno et al GM Crops Food. 2015;6(4):243-52. doi: 10

Insert

Glycine Max codon optimized Cas9

Use

CRISPR

Expression

Plant

Promoter

G10

Available Since

Dec. 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.0 FLAG TRPV4

Plasmid

#45751

Depositor

Robert Lefkowitz

Article

Shukla et al J Biol Chem. 2010 Sep 24;285(39):30115-2

Insert

TRPV4 (Trpv4 Rat)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
dSpCas9-VPR

Plasmid

#205247

Purpose

In vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.

Depositor

Rasmus Bak

Article

Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

Insert

dSpCas9-VPR

Use

CRISPR

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSBtet-Hygro-GADD34_Δ1-240/P2A/K3L

Plasmid

#196136

Purpose

SB vector for the mammalian expression of GADD34-K3L

Depositor

Jamie Cate

Article

Aleksashin et al bioRxiv. 2023 Feb 10:2023.02.09.527910.

Insert

GADD34 Δ1-240 - P2A - K3L (PPP1R15A Human, vaccinia virus)

Tags

P2A

Expression

Mammalian

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge