We narrowed to 28,500 results for: REP

1. pdnCas3-CDA1

   Plasmid

   #229535

   Purpose

   pMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

   Depositor

   Kevin Verstrepen

   Article

   Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

   Inserts

   dnCas3
   Cytidine deaminase
   Uracil glycosylase inhibitor
   

   Use

   Tags

   XTEN

   Expression

   Bacterial and Yeast

   Mutation

   codon optimized for S.cerevisiae and codon optimi…

   Promoter

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pdnCas3-APOBEC1

   Plasmid

   #229537

   Purpose

   pMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

   Depositor

   Kevin Verstrepen

   Article

   Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

   Inserts

   rAPOBEC1
   dnCas3
   Uracil glycosylase inhibitor
   

   Use

   Tags

   XTEN

   Expression

   Bacterial and Yeast

   Mutation

   codon optimized for S.cerevisiae and codon optimi…

   Promoter

   Available since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUC19-π-EB1_IRES-mCherry

   Plasmid

   #197427

   Purpose

   Homology-directed repair template targeting IRES-based π-element to MAPRE1 exon 5

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   IRES-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GCN4 leucine zipper, LOV2, Zdk1, and mCherry

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-π-EB1_EF1α-mCherry

   Plasmid

   #197429

   Purpose

   Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GCN4 leucine zipper, LOV2, Zdk1, and mCherry

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUC19-π-EB1_EF1α-EGFP

   Plasmid

   #197430

   Purpose

   Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   EGFP, GCN4 leucine zipper, LOV2, and Zdk1

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJET-col2a1a-KI-donor

   Plasmid

   #184876

   Purpose

   Donor template for knock-in of p2a-CreERT2 into the medaka col2a1a locus via CRISPR/Cas9-mediated homology directed repair

   Depositor

   Christoph Winkler

   Article

   Hui et al Development. 2022 May 20. pii: 275483. d

   Inserts

   Collagen2a1a 5' homology arm
   Collagen2a1a 3' homology arm
   p2a-CreERT2
   Myosin light chain 2 promoter
   EGFP
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSMT3-FBF2 SS/Y

   Plasmid

   #166973

   Purpose

   Expresses FBF-2 with SS/Y mutations in repeat 5 in E. coli for purification and crystallization studies

   Depositor

   Traci Hall

   Article

   Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

   Insert

   FBF-2 (fbf-2 Nematode)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   FBF-2 PUM RNA binding domain; amino acids 164-575…

   Promoter

   T7

   Available since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSMT3-FBF2 AS/Y

   Plasmid

   #166974

   Purpose

   Expresses FBF-2 with AS/Y mutations in repeat 5 in E. coli for purification and crystallization studies

   Depositor

   Traci Hall

   Article

   Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

   Insert

   FBF-2 (fbf-2 Nematode)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   FBF-2 PUM RNA binding domain; amino acids 164-575…

   Promoter

   T7

   Available since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHR-HOXD13(+7A)-IDR-YFP-NLS

   Plasmid

   #145278

   Purpose

   Mammalian Expression of Nuclear HOXD13 (+7A) IDR-YFP with SV40 NLS

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXD13(+7A)-IDR (HOXD13 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A-repeat expanded

   Promoter

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-45b-HOXA13(+7A)-IDR-mCherry

    Plasmid

    #145285

    Purpose

    Bacterial Expression of 6xHis HOXA13(+7A)IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXA13(+7A)-IDR (HOXA13 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A-repeat expanded

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCFP-LacI-HOXD13(+10A)-IDR

    Plasmid

    #145257

    Purpose

    Expresses fusion of ECFP-LacI and HOXD13+10A IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13(+10A)-IDR (HOXD13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeat expanded

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFP-LacI-HOXD13(+7A)-IDR

    Plasmid

    #145256

    Purpose

    Expresses fusion of ECFP-LacI and HOXD13+7A IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13(+7A)-IDR (HOXD13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeat expanded

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGAL4-DBD-HOXD11-Adel-IDR

    Plasmid

    #145241

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and HOXD11-Adel IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD11-Adel-IDR (HOXD11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeats deleted

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGAL4-DBD-HOXA13+7A-IDR

    Plasmid

    #145239

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and HOXA13+7A IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXA13+7A-IDR (HOXA13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeat expanded

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGAL4-DBD-EOMES-Adel-IDR

    Plasmid

    #145230

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and EOMES-Adel IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    EOMES-Adel-IDR (EOMES Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeats deleted

    Promoter

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGAL4-DBD-GSX2-Adel-IDR

    Plasmid

    #145232

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and GSX2-Adel IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    GSX2-Adel-IDR (GSX2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeats deleted

    Promoter

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFUW-tetO-KLF4

    Plasmid

    #139828

    Purpose

    doxycycline-inducible expression of Human KLF4 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. U6_sgRNA_CAG_hSt1Cas9_MTH17CL396

    Plasmid

    #136656

    Purpose

    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:MTH17CL396 chimera) and its U6-driven sgRNA.

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9:MTH17CL396 Chimera
    sgRNA for St1Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and hU6

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. U6_sgRNA_CAG_hSt1Cas9_TH1477

    Plasmid

    #136655

    Purpose

    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:TH1477 chimera) and its U6-driven sgRNA.

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9:TH1477 Chimera
    sgRNA for St1Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and hU6

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. U6_sgRNA_CAG_hSt1Cas9_LMG18311

    Plasmid

    #136653

    Purpose

    A single vector mammalian expression system containing a CAG promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD9:LMG18311 chimera) and its U6-driven sgRNA.

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD9:LMG18311 chimera
    sgRNA for St1Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and hU6

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge