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Plasmid#224447PurposeRep/Cap plasmid for the production of MyoAAV 3F, a muscle-tropic AAV capsid in mice and macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVMutationRGDHASW insert between amino acids 588 and 589 of…Promoterp41Available SinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-MyoAAV3A
Plasmid#224442PurposeRep/Cap plasmid for the production of MyoAAV 3A, a muscle-tropic AAV capsid in mice and macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVMutationRGDYVGL insert between amino acids 588 and 589 of…Promoterp41Available SinceSept. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNeonGreen-C1-Nir2-LNS2
Plasmid#220086PurposeC-terminal PA binding domain of Nir2DepositorInsertPITPNM1 (PITPNM1 Human)
TagsNeonGreen-GGSGGMExpressionMammalianMutationamino acids 896-1216PromoterCMVAvailable SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGKCMV)-nucTomato-hygro
Plasmid#208573PurposeLV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an PGK/CMV promoter driving nuclear d2eGFP E2A HygromycinDepositorInsertsmnucTomato
d2nucEGFP
HygroR
UseLentiviral and Synthetic BiologyExpressionMammalianPromoterPGK/CMV and minPAvailable SinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-DAG1
Plasmid#205149PurposeOverexpress DAG1DepositorInsertDystroglycan 1 (DAG1 Human)
UseLentiviralExpressionMammalianMutationCarries common missense variant c.41C>G (p.Ser…PromoterEF-1aAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-PARP1-Apex2–eGFP
Plasmid#211555PurposeA piggyBac vector containing CMV-PARP1-Apex2-eGFP-IRES-Neo cassette.DepositorAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNKX2-5-T2A-GFP-PGK-Puro
Plasmid#203607PurposeDonor plasmid to create 3' knockin of NKX2-5 with T2A-GFPDepositorInsertNKX2-5-T2A-GFP-PGK-Puro-NKX2-5 (NKX2-5 Human)
UseHuman targetingAvailable SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP777_SnoopCatcher-p65HSF1-CO
Plasmid#211770PurposeSnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional activator p65HSF1, with GFP selectionDepositorInsertSnoopCatcher-p65HSF1
Tags2xOLLASExpressionMammalianMutationLast 300 bp codon-optimised (CO) to detect p65HSF…PromoterpEF1a and pSV40Available SinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW-GemininΔDbox-HA-Puro
Plasmid#193770PurposeDoxycyline-inducible (TetOn) human Geminin (C-terminal HA tag) with Dbox removed to prevent degradation by APC/C, expressed from all-in-one pCW vector (TetOn + rtTA)DepositorInsertGemininΔDbox-HA (GMNN Human)
UseLentiviralTagsHAMutationDbox removed (R23A, L26A). Codons switched at siR…PromoterTREAvailable SinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
BcLOV4 fusion tools screening plasmid #2
Plasmid#174512PurposeCloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP in a pcDNA3.1 backbone.DepositorInsertplasmid B [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP
Tags3xFLAG and mCherryExpressionMammalianPromoterCMVAvailable SinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
betaTrCP mVenus mutant reporter
Plasmid#192459PurposeFluorescent reporter for beta-TrCP with a non-degradable mutation. Used as a control.DepositorInsertCdC25B (CDC25B Human)
UseLentiviralTagsSV40 NLS and mVenusExpressionMammalianMutationaa198-338 of CDC25B. betaTrCP degron sequence (aa…PromoterEF1alphaAvailable SinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-HNF1B-WT-Flag-UTR
Plasmid#183239PurposeExpression of FLAG tagged HNF1BDepositorAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJT304 Ec07 Lenti pEF-attB LSR GFP
Plasmid#184947PurposeLentiviral delivery of recombinase landing padDepositorInsertEc07 Lenti pEF1a-attB LSR GFP
UseLentiviral and Synthetic BiologyExpressionMammalianAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
canxTM-mEmerald (cytosolic)
Plasmid#186962PurposeExpresses mEmerald connected with the C-terminus of Calnexin transmembrane domain.DepositorInsertCANX (CANX Human)
TagsmEmeraldExpressionMammalianMutationOnly express calnexin (482-end) and add ER signal…PromoterCMV PromoterAvailable SinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
GLI1_pLENTI-CAG-IRES-GFP
Plasmid#176992PurposeMammalian lentiviral expression vector encoding GLI1DepositorAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Anti-Npas4 [N408/79]
Plasmid#177547PurposeMammalian Expression Plasmid of anti-Npas4 (Rat). Derived from hybridoma N408/79.DepositorInsertanti-Npas4 (Rattus norvegicus) recombinant mouse monoclonal antibody (Npas4 Mouse)
ExpressionMammalianPromoterDual CMVAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiDP1.2
Plasmid#171095Purposelentiviral construct (3rd generation) for the expression of cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (AKA ROLECCS G2) and miniAID-fused mCherry2DepositorInsertOsTIR1-mEmerald-Geminin-P2A-miniAID-mCherry2
UseLentiviral and Synthetic BiologyTagsEmerald, Geminin, miniAID, mCherry2ExpressionMammalianPromoterCMVAvailable SinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRI009-pKW20088-PelcA-mCherry-TtrpC-NotI-PacI
Plasmid#140202PurposeCRISPRa proof-of-concept test target with fluorescent reporter. PelcA is fused to mCherry, with low basal expression in Aspergillus nidulans. Fungal vector with AMA1 and pyrG selection marker.DepositorInsertmCherry
UseCRISPR and Synthetic Biology; Proof-of-concept te…MutationG174DPromoterPelcAAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_GP1Fc Cl13
Plasmid#138599PurposeExpresses GP1 of LCMV Cl13 fused with human IgG (Fc fusion)DepositorInsertLCMV GP1-Fc
TagsFc fusion proteinExpressionMammalianPromoterCMVAvailable SinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP
Plasmid#135766PurposeThis plasmid can be used to express independently eGFP and a zebrafish wt1b dominant negative isoform (N-terminal domain) from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.DepositorAvailable SinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only