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mNeonGreen-mTurquoise2

Plasmid

#98886

Purpose

Produces a fusion between mTurquoise2 and mNeonGreen. It can be used as a positive control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mNeonGreen-mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLPCX cyto Grx1-roGFP2

Plasmid

#64975

Purpose

Mammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)

Depositor

Tobias Dick

Article

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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pAF0036 Ef1a-Si74-2a-EGFP

Plasmid

#193463

Purpose

Expression of Si74 recombinase from Ef1a promoter

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Si74

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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PB-TO-NGN2-EMX1 BSD-mApple

Plasmid

#182311

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 and EMX1 expression, bsd selection, mApple

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Inserts

hNGN2-P2A-EMX1 (NEUROG2, EMX1 Human)
bsd-T2A-mycNLS-mApple

Tags

T2A-mycNLS-mApple

Expression

Mammalian

Promoter

EF1a and TRE3G

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1ab-Prkd1

Plasmid

#139314

Purpose

Diacylglycerol biosensor

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

mCherry:Mus musculus Prkd1(138-343) (Prkd1 Mouse)

Expression

Mammalian

Mutation

T208A, N313D

Available Since

April 8, 2020

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-OSKM

Plasmid

#20321

Purpose

Lentiviral plasmid for tet-inducible expression of mouse Oct4, Sox2, Klf4 and Myc for iPS cell generation

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Oct-4 (Pou5f1, Sox2, Klf4, Myc Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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MCS-TurboID MSCV-IRES-GFP

Plasmid

#207957

Purpose

Retroviral vector for cloning a gene of interest with a glycine-serine linker and TurboID on the C-terminus

Depositor

Timothy Ley

Article

Day et al J Clin Invest. 2023 Dec 7:e176311. doi:

Insert

TurboID

Use

Retroviral; Turboid biotin proximity ligase

Tags

TurboID

Expression

Mammalian

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAK005

Plasmid

#233642

Purpose

For bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagB.

Depositor

Zak Swartz

Article

Clarke et al Development. 2024 Oct 15;151(20):dev2028

Insert

eSpCas9(1.1) with c-terminal VitelloTagB

Tags

6-His

Expression

Mammalian

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hSTING (R232)

Plasmid

#214152

Purpose

Transient expression of STING R232

Depositor

Nan Yan

Article

Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

Insert

STING (STING1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLX302_FOXA1-V5

Plasmid

#70090

Purpose

Expression of human FOXA1 with C-terminal V5 tag, puromycin selection marker, 3rd generation lentiviral vector

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

FOXA1 (FOXA1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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