4,076 results

1. pLEX_305-N-dTAG

   Plasmid

   #91797

   Purpose

   Lentiviral/gateway cloning vector for N-terminally tagging proteins of interest with the dTAG system

   Depositor

   James Bradner, Behnam Nabet

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   FKBP F36V

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBOB-EF1-FastFUCCI-Puro

   Plasmid

   #86849

   Purpose

   3rd generation lentiviral vector encoding FUCCI reporters for cell cycle analysis and puro for selection

   Depositor

   Duncan Jodrell, Kevin Brindle

   Article

   Koh et al J Cell Sci. 2016 Nov 25. pii: jcs.195164

   Inserts

   mKO2-hCDT1(30-120)
   mAG-hGEM(1-110)
   pac (puromycin N-acetyl-transferase)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1 and PGK

   Available since

   Nov. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-TetOne-Puro-GFP

   Plasmid

   #171123

   Purpose

   Doxycycline inducible expression of GFP

   Depositor

   Jason Sheltzer

   Article

   Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-Foxo1 (pCMV5)

   Plasmid

   #12142

   Depositor

   Domenico Accili

   Article

   Nakae et al J Clin Invest. 2001 Nov . 108(9):1359-67

   Insert

   Foxo1 (Foxo1 Mouse)
   

   Use

   Tags

   HA and Myc

   Expression

   Mammalian

   Mutation

   Wild-type

   Promoter

   CMV

   Available since

   Aug. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9n(BB)-2A-Puro (PX462) V2.0

   Plasmid

   #62987

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Available since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

   Plasmid

   #139998

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

   Promoter

   CAG

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

   Plasmid

   #190034

   Purpose

   vector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promoters

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

   Insert

   humanized hfCas13d
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A134V,A140V,A141V,A143V

   Promoter

   CBh, SV40, hU6

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-GFP

   Plasmid

   #32395

   Depositor

   John T Gray

   Article

   Gray et al Methods Mol Biol. 2011;807:25-46.

   Insert

   GFP
   

   Use

   AAV; Adeno-associated virus

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-CreERT2

   Plasmid

   #14797

   Purpose

   Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   Cre-ERT2 fusion protein
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. HRE-luciferase

    Plasmid

    #26731

    Purpose

    Luciferase reporter construct containing three hypoxia response elements (24-mers) from the Pgk-1 gene.

    Depositor

    Navdeep Chandel

    Article

    Emerling et al Proc Natl Acad Sci U S A. 2008 Feb 19. 1

    Insert

    Hypoxia Responsive Elements (3)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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