57,916 results
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Plasmid#24317DepositorInserthuman AGO2 (AGO2 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
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AARS_C2_pNIC-Bio3
Plasmid#153047PurposeExpression construct for producing Avi tagged Alanyl-tRNA synthetaseDepositorInsertAARS (AARS Human)
UseTagsAvi and His6-TEVExpressionBacterialMutationPromoterT7Available sinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMVHA E2F1
Plasmid#24225PurposeMammalian expression of human E2F1 with HA tagDepositorInsertE2F1 (E2F1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hM3D(Gq)-mCherry
Plasmid#50474PurposeGq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoterDepositorUseAAVTagsmCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
856 pBabe puroL PTEN C124S
Plasmid#10931DepositorInsertPTEN (PTEN Human)
UseRetroviralTagsExpressionMammalianMutationC124SPromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCDFDuet-1 MKK4(EE)-MKK7a1(EE)
Plasmid#47580PurposeBacterial expression plasmid for co-expression of constitutively active MKK4 and MKK7a1DepositorUseTagsExpressionBacterialMutationLacks the first 36 residues of the RefSeq protein…PromoterAvailable sinceSept. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-15b_LC3B
Plasmid#73949PurposeFor recombinant expression of human LC3B/MAP1LC3B in E. coliDepositorInsertLC3B (MAP1LC3B Human)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-hNGN2
Plasmid#172115PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expressionInsertsUseTagsT2A-mycNLS-mTagBFP2ExpressionMammalianMutationPromoterEF1a and TRE3GAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTK168_mCherry-H2B
Plasmid#46363Purposevisualizes chromosomesDepositorInsertH2B (H2BC11 Human)
UseRetroviralTagsmCherryExpressionMammalianMutationD26G, V119I and S125DPromoterpBABEAvailable sinceAug. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGS_101
Plasmid#160541PurposeExpresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoterDepositorInsertApolipoprotein E e4 (APOE Human)
UseTagsKar2 signal sequenceExpressionYeastMutatione4 allelePromoterGAL1Available sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-tet-iCas9-BFP2
Plasmid#125519Purposeall-in-one-inducible Cas9DepositorInsertAAVS1-TET-ON 3G-SpCas9-P2A-BFP2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
myc-Rictor corrected
Plasmid#11367PurposeMammalian expression of myc tagged human RICTORDepositorInsertRictor (RICTOR Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceApril 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pXR002: EF1a-dCasRx-2A-EGFP
Plasmid#109050PurposeCatalytically inactive dCasRx with 2A-EGFPDepositorInsertdCasRx
UseCRISPR and LentiviralTags2A-eGFP, HA, and NLSExpressionMammalianMutationR239A/H244A/R858A/H863APromoterAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
mClY-N1
Plasmid#90457PurposeA YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.DepositorInsertmClY-N1
UseTagsExpressionMammalianMutationEYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…PromoterAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
LPAR1-Tango
Plasmid#66418PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertLPAR1 (LPAR1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO1-shYAP1
Plasmid#27368DepositorInsertshYAP1
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
FLAG-hMYOD1 (#794)
Plasmid#78329PurposeOverexpression of human MYOD1 ORF with a N-terminal FLAG tagDepositorInsertMYOD1 (MYOD1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX_TRC311/NLS-Cas13d-P2A-Blast
Plasmid#212196PurposeCasRx (NLS-RfxCas13d-NLS) with 2A-Blasticidin for RNA targeting. CasRx-2A-Blasticidin is driven by EF1a promoter. EGFP is driven by SV40DepositorInsertCasRx (NLS-RfxCas13d-NLS)-HA with 2A-Blasticidin
UseCRISPR and LentiviralTagsHA, NLS, and blasticidin S deaminaseExpressionMammalianMutationPromoterEF-1α promoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCW-HA-hRb-WT-puro
Plasmid#212672PurposeExpress tagged hRbDepositorInsertRb (RB1 Human)
UseLentiviralTagsHAExpressionMutationPromoterTREAvailable sinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only