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We narrowed to 2,855 results for: BFP

Showing: 1381 - 1410 of 2855 results

  3. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR BFP-guide1

    Plasmid
    #118161
    Purpose
    CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA1 against the BFP CDS
    Depositor
    Jorge Ferrer
    Article
    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK and U6
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118161-plasmid-map-sequence-id-240357

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  9. pHBS1202 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 4xΦ]

    Plasmid
    #107845
    Purpose
    To test the effect of sequence on TDP43 splicing activity
    Depositor
    Rajat Rohatgi
    Insert
    TARDBP mutant (TARDBP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    All hydrophobics combined in four clusters in spl…
    Promoter
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    107845-plasmid-map-sequence-id-203697

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  10. pHBS1203 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 2xΦ]

    Plasmid
    #107844
    Purpose
    To test the effect of sequence on TDP43 splicing activity
    Depositor
    Rajat Rohatgi
    Insert
    TARDBP mutant (TARDBP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    All hydrophobics combined in two clusters in spli…
    Promoter
    Available since
    April 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    107844-plasmid-map-sequence-id-203696

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  12. pCFJ2245 - [1-2] ENTR - Fluor - ce-tagBFP2 (1 intron, syntrons(3), no_atg, no_stop)

    Plasmid
    #159843
    Purpose
    Three-fragment Gateway compatible flourophore for expression in C. elegans
    Depositor
    Christian Froekjaer-Jensen
    Article
    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:
    Insert
    [1-2] ENTR - Fluor - ce-tagBFP2 (1 intron, syntrons(3), no_atg, no_stop)
    Use
    Tags
    Expression
    Worm
    Mutation
    Not applicable
    Promoter
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159843-plasmid-map-sequence-id-317994

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  13. pEJS2783 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-16 CMV-BFP

    Plasmid
    #240513
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Use
    Tags
    FLAG, hsa-miR-16 Target Sites, and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240513-plasmid-map-sequence-id-477190

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  16. pEJS1354 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-1 CMV-BFP

    Plasmid
    #226113
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Use
    Tags
    FLAG, hsa-miR-1-5p Target Sites, and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    226113-plasmid-map-sequence-id-448319

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  17. pEJS1352 CMV-AcrIIC3-FLAGNLS-mCherry-3xmiR-22 CMV-BFP

    Plasmid
    #226111
    Purpose
    Mammalian expression of AcrIIC3-mCherry fusion with microRNA target sites in the 3' UTR as a reporter of microRNA repression. BFP expressed separately as a transfection control.
    Depositor
    Erik Sontheimer
    Article
    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10
    Insert
    AcrIIC3
    Use
    Tags
    FLAG, hsa-miR-22 Target Sites, and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    226111-plasmid-map-sequence-id-448317

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  29. FUW-crRNA-1-U6_TRE-dLbCpf1-NLS-hCTCF_K365A_R368A-HA-P2A-tagBFP

    Plasmid
    #194890
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-K365A_R368A mutant and crRNA-1 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_K365A_R368A
    U6-crRNA1
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available since
    May 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    194890-plasmid-map-sequence-id-392583

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  30. FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_Q418A-HA-P2A-tagBFP

    Plasmid
    #194895
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-Q418A mutant and crRNA-2 targeting the human MECP2 locus.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_Q418A
    U6-crRNA2
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available since
    April 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    194895-plasmid-map-sequence-id-389971

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Showing: 1381 - 1410 of 2855 results