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Plasmid#236079PurposeEmpty lentiviral backbone with N-terminal HA tagDepositorTypeEmpty backboneUseLentiviralTagsHAExpressionMutationPromoterCMVAvailable sinceApril 25, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits
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pAG Lenti CMV C-HA Puro
Plasmid#236080PurposeEmpty lentiviral backbone with C-terminal HA tagDepositorTypeEmpty backboneUseLentiviralTagsHAExpressionMutationPromoterCMVAvailable sinceApril 25, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti Puro HA-Ubiquitin-K48
Plasmid#236290PurposeLentiviral vector expressing HA-tagged ubiquitin K48 mutantDepositorInsertUbiquitin K48 (UBB Human)
UseLentiviralTagsHAExpressionMutationSingle ubiquitin coding sequence containing K48 m…PromoterCMVAvailable sinceApril 10, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti Puro HA-Ubiquitin-K63
Plasmid#236291PurposeLentiviral vector expressing HA-tagged ubiquitin K63 mutantDepositorInsertUbiquitin K63 (UBB Human)
UseLentiviralTagsHAExpressionMutationSingle ubiquitin coding sequence containing K63 m…PromoterCMVAvailable sinceApril 10, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLKO.1-puro_CMV_NES-Kinprola_PKA-mEGFP
Plasmid#233369PurposeCMV driven expression of the PKA activity recorder Kinprola_PKA fused to mEGFP for neuronal expression through lentivirus transductionDepositorInsertNES-Kinprola_PKA-mEGFP
UseLentiviralTagsNES and mEGFPExpressionMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro_CMV_NES-Kinprola_on-mEGFP
Plasmid#233370PurposeCMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP for neuronal expression through lentivirus transductionDepositorInsertNES-Kinprola_on-mEGFP
UseLentiviralTagsNES and mEGFPExpressionMutationPromoterCMVAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG Retro EGFP Puro
Plasmid#234463PurposeControl retroviral vector expressing Green Fluorescent Protein. Puromycin selection.DepositorInsertGreen Fluorescent Protein
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMarch 21, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pB-Puro-DEST (JDW 925)
Plasmid#229808PurposeA puromycin selectable, PiggyBac destination vector compatible with 4-way multi-site gateway cloning systemDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-Dpi2Cas9-puro
Plasmid#232113PurposeExpresses Dpi2Cas9 and puromycin resistance genes, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-Slu1Cas9-puro
Plasmid#232111PurposeExpresses Slu1Cas9 and puromycin resistance genes, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-Slu2Cas9-puro
Plasmid#232112PurposeExpresses Slu2Cas9 and puromycin resistance genes, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-SeqCas9-puro
Plasmid#232110PurposeExpresses SeqCas9 and puromycin resistance genes, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pdest myr-AKT2 puro
Plasmid#223053Purposemyr-AKT2 gene expression in mammalian cellsDepositorInsertmyr-AKT2 (Akt2 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pdest N-FLAG METTL1-WT puro
Plasmid#223055PurposeMETTL1-WT gene expression in mammalian cellsDepositorInsertMETTL1-WT (METTL1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pdest N-FLAG EGFP puro
Plasmid#223056PurposeEGFP gene expression in mammalian cellsDepositorInsertEGFP
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CRISPRv2-sgNTC49-puro
Plasmid#231989PurposeKnockout non-targeting controlDepositorInsertsgRNA with Cas9 and hygromycin resistance
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-mGFP-FXR1-N2 (1-399)
Plasmid#225452PurposeExpress mEGFP-fusion protein of FXR1 fragment (1-399)DepositorInsertFXR1 (FXR1 Human)
UseTagsmEGFPExpressionMammalianMutationN2 fragment (1-399)PromoterCMVAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-mGFP-FXR1-N1 (1-379)
Plasmid#225450PurposeExpress mEGFP-fusion protein of FXR1 fragment (1-379)DepositorInsertFXR1 (FXR1 Human)
UseTagsmEGFPExpressionMammalianMutationN1 fragment (1-379)PromoterCMVAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMV Puro DEST P38KTRDronpa
Plasmid#205763PurposeExpression of P38 KTR Dronpa under CMV promoter (With Puromycin Resistance)DepositorInsertP38 KTR Dronpa
UseLentiviralTagsExpressionMammalianMutationwtPromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Puro DEST JNKKTRrsGreenF-E
Plasmid#205764PurposeExpression of JNK KTR rsGreenF-E under PKG promoter (With Puromycin Resistance)DepositorInsertJNK KTR rsGreenF-E
UseLentiviralTagsExpressionMammalianMutationwtPromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCMV Puro DEST ERKKTRrsFastLime
Plasmid#205765PurposeExpression of ERK KTR rsFastLime under CMV promoter (With Puromycin Resistance)DepositorInsertERK KTR rsFastLime
UseLentiviralTagsExpressionMammalianMutationwtPromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-mGFP-FMR1iso1-N1(1-445)
Plasmid#225464PurposeExpress mEGFP-fusion protein of FMR1 fragment (1-445)DepositorInsertFMR1 (FMR1 Human)
UseTagsmEGFPExpressionMammalianMutationN1 fragment (1-445)PromoterCMVAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-mGFP-FMR1iso1-N2(1-491)
Plasmid#225465PurposeExpress mEGFP-fusion protein of FMR1 fragment (1-491)DepositorInsertFMR1 (FMR1 Human)
UseTagsmEGFPExpressionMammalianMutationN2 fragment (1-491)PromoterCMVAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
3xMS-TR-100-PURO HRD
Plasmid#207582Purposehomologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locusDepositorInsert3xMS2-TR and 100 bp of downstream sequence followed by a PuroR and flanked by endogenous TR sequences
UseTags3xMS2ExpressionMammalianMutationPromoterSV40Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR v2 puro mGSDME gRNA2
Plasmid#223522PurposeKnocking out mGSDME in mouse cellsDepositorInsertGSDME (GSDME Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPR v2 puro mGSDME gRNA1
Plasmid#223521PurposeKnocking out mGSDME in mouse cellsDepositorInsertGSDME (GSDME Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
CROP-seq-puro-v2-no-scaffold
Plasmid#228521PurposeCROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequencesDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAS_pb_Ef1a-TIR1_Ubc-mCherry-Puro
Plasmid#195567PurposePiggyBac plasmid for stable expression of TIR1, mCherry, and Puromycin resistance in mammalian cellsDepositorInsertTIR1
UseTagsExpressionMammalianMutationPromoterEf1-alphaAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-mStayGold-LMNB1
Plasmid#227329PurposeDonor template for Puro-2A-mStayGold insertion into the N-terminus of the LMNB1 locus. For nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 (Addgene #207770)DepositorInsertLMNB1 Homology Arms flanking a Puro-2A-mStayGold (LMNB1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-TRE-miRFP703_PGK-Puro
Plasmid#216667PurposeExpresses tTa-inducible miRFP703, with puromycin resistanceDepositorInsertsmiRFP703
PuroR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mStayGold-Puro-H2BC11
Plasmid#227332PurposeDonor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)DepositorInsertH2BC11 Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-mStayGold-TUBA1B
Plasmid#227321PurposeDonor template for Puro-2A-mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)DepositorInsertTUBA1B Homology Arms flanking a Puro-2A-mStayGold Cassette (TUBA1B Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL2368 MTK PB puro backbone
Plasmid#226673PurposeEmpty MTK PB cargo backbone marked with puromycin resistance.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYX-N-Puro-mNeon-TSC22D1
Plasmid#222667Purposeendogenously tag TSC22D1 with N-terminal mNeonDepositorInsertTSC22D1 (TSC22D1 Human)
UseTagsmNeonExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-mGFP-FXR1a-Ccswap
Plasmid#225460PurposeExpress EGFP-fusion protein of FXR1 isoform a (CCswap)DepositorInsertFXR1 isoform a (FXR1 Human)
UseTagsEGFPExpressionMammalianMutationCCswap (swapped positions of CC2 and CC1)PromoterCMVAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-EGFP-GAPDH-R rich
Plasmid#225480PurposeExpress mEGFP-GAPDH-Rrich fusionDepositorInsertGAPDH fused with R-rich region of TDRD3 (GAPDH Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-mGFP-FXR1a-I304N
Plasmid#225461PurposeExpress mEGFP-fusion protein of FXR1 isoform a (I304N)DepositorInsertFXR1 isoform a (FXR1 Human)
UseTagsmEGFPExpressionMammalianMutationI304NPromoterCMVAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-EGFP-GAPDH-Tudor
Plasmid#225479PurposeExpress mEGFP-GAPDH-Tudor fusionDepositorInsertGAPDH fused with Tudor domain of TDRD3 (GAPDH Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-EGFP-GAPDH-RGG
Plasmid#225477PurposeExpress mEGFP-GAPDH-RGG fusionDepositorInsertGAPDH fused with RGG domain of TOP3B (GAPDH Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS puro 3XFLAG-hATXN7L3
Plasmid#225725PurposeTransfection of ATXN7L3 with 3XFLAG tag in mammalian cellsDepositorInsertATXN7L3 (ATXN7L3 Human)
UseTags3XFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only