We narrowed to 7,571 results for: Lif

1. pKL09-GCaMP6f-mScarlet bb118

   Plasmid

   #158979

   Purpose

   Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.

   Depositor

   Joel Kralj

   Article

   Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

   Insert

   GCaMP6f-mScarlet fusion
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   codon optimized for e. coli from Addgene#100833

   Promoter

   Bba_J23118 (sigma 70)

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHFS:SEP-rGluA1 TKIT donor

   Plasmid

   #169438

   Purpose

   Donor DNA for TKIT knockin of SEP to GluA1 in rat

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   Super ecliptic pHluorin (SEP)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. NES-mTagBFP2-P4Mx1

   Plasmid

   #108142

   Purpose

   PI4P biosensor

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   X.leavis map2k1.L(32-44):mTagBFP2:L. penomophila SidM(546-647)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-LambdaN-NbALFA

   Plasmid

   #207627

   Purpose

   Expresses LambdaN-NbALFA (ALFA-tag nanobody) fusion protein in mammalian cells (with a CMV promoter)

   Depositor

   Jay Shendure

   Article

   Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

   Insert

   LambdaN-NbALFA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   pCMV

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-RILP

   Plasmid

   #74600

   Purpose

   used for expression in E. coli and purification

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   Rab-interacting lysosomal protein (RILP Human)
   

   Use

   Tags

   FLAG, StrepII, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-mTagBFP2-TEV-Strep-His

   Plasmid

   #210824

   Purpose

   Bacterial overexpression vector for C-terminally Strep and His tagged mTagBFP2 fluorescent protein

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   mTagBFP2
   

   Use

   Tags

   TwinStrep, 10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMiniT:SEP-GluA1 TKIT donor

   Plasmid

   #169418

   Purpose

   Donor DNA for TKIT knockin of SEP to GluA1 in mouse

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   Super ecliptic pHluorin (SEP)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. epB_CAG_TetRFlag-nls-tdTom_DEx4

   Plasmid

   #119909

   Purpose

   ePiggyBac mammalian expression plasmid for TetR-tdTom fusion

   Depositor

   Orion Weiner

   Article

   Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

   Insert

   TetRFlag-nls-tdTom
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-FLAG-R-Ras2

   Plasmid

   #102335

   Purpose

   Human R-Ras2 with FLAG tag for mammalian expression

   Depositor

   Hening Lin

   Article

   Zhang et al Elife. 2017 Apr 13;6. pii: e25158. doi:

   Insert

   RRAS2 (RRAS2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBTK1024 (pDS-Def1)

    Plasmid

    #183125

    Purpose

    Expresses double-stranded RNA targeting the Def1 gene from Apis mellifera

    Depositor

    Jeffrey Barrick

    Article

    Lariviere et al Nat Protoc. 2023 Mar;18(3):902-928. doi:

    Insert

    Fragment of Def1 gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. V5-TurboID-Mfn1E209A

    Plasmid

    #195156

    Purpose

    mammalian expression of TurboID tagged Mfn1E209A

    Depositor

    Gia Voeltz

    Article

    Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

    Insert

    TurboID
    

    Use

    Tags

    Mfn1-E209A

    Expression

    Mammalian

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Promoter

    CMV

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJCC_083 Cas12i1

    Plasmid

    #171670

    Purpose

    For bacterial expression of Cas12i1 with an N-terminal His-MBP tag

    Depositor

    Jennifer Doudna

    Article

    Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

    Insert

    Cas12i1
    

    Use

    Tags

    10X His, TEV protease cleavage site, and maltose-…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDRFLIP38

    Plasmid

    #65516

    Purpose

    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-edCit-attR1 and attR2-edCer-cMyc

    Expression

    Yeast

    Mutation

    Promoter

    pPMA1

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEF-mRuby3

    Plasmid

    #179730

    Purpose

    Mammalian expression of mRuby3 from the EF1 promoter

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    mRuby3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2+Paxillin-mKate

    Plasmid

    #105974

    Purpose

    in vitro transcription, paxillin coding sequence without stop codon-paxilin is a protein of focal adhesions

    Depositor

    Caren Norden

    Article

    Sidhaye et al Elife. 2017 Apr 4;6. pii: e22689. doi: 1

    Insert

    Paxillin
    

    Use

    Tags

    mKate

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Nsp2Cas9_AAV

    Plasmid

    #192143

    Purpose

    Expresses Nsp2Cas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

    Insert

    Nsp2Cas9
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET303-colE1TR

    Plasmid

    #155181

    Purpose

    Expresses colicin E1 residues 1-364

    Depositor

    Joanna Slusky

    Article

    Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

    Insert

    colicin E1 residues 1-364
    

    Use

    Tags

    C-terminal 6x Histidine tag

    Expression

    Bacterial

    Mutation

    truncation of colicin E1 including 1-364

    Promoter

    T7

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCSIIbsr-GCaMP6s

    Plasmid

    #184712

    Purpose

    Calcium biosensor

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

    Insert

    GCaMP6s
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pN3-mTagBFP-pHluorin-CAAX

    Plasmid

    #178345

    Purpose

    Express a pH insensitive blue fluorencent protein mTagBFP fused to the N-terminal of a membrane targeted pH sensitive green fluorescent protein pHluorin-CAAX under CMV promoter

    Depositor

    Yulong Li

    Article

    Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

    Insert

    mTagBFP-pHluorin-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDK521

    Plasmid

    #134204

    Purpose

    Baculovirus expresion of Astrin, LC8, and SKAP

    Depositor

    Iain Cheeseman

    Article

    Kern et al Elife. 2017 Aug 25;6. pii: 26866. doi: 1

    Insert

    Astrin 1-693-sGFP-His BroAstrin and LC8 and AltSKAP in pACE1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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