We narrowed to 4,416 results for: Pet2

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pET21a-CysRS-His

Plasmid

#124107

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Cysteinyl-tRNA ligase (cysS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET24-ISWI-iCBD

Plasmid

#105499

Purpose

Expression of Drosophila ISWI C-terminally tagged with Combined Sce VMA intein and chitin binding domain (iCBD)

Depositor

James Kadonaga

Article

Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

Insert

ISWI-iCBD

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-4

Plasmid

#172770

Purpose

Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-4

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(41-140)

Plasmid

#240596

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 41-140 only

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET21a asyn(36-140)

Plasmid

#240595

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 36-140 only

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pET28.His.3C.86b.Tau2N4R

Plasmid

#226399

Purpose

Expresses his-tagged, 3C-cleavable, 86b (split luciferase fragment)-tagged WT 2N4R tau for recombinant protein production in bacteria

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT Human, synthetic)

Tags

His.3C.86b

Expression

Bacterial

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pET21b Hot-PETase

Plasmid

#225761

Purpose

Expresses codon-optimized Hot-PETase in E. coli with a C-terminal his-tag

Depositor

Jason Boock

Article

Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

Insert

Hot-PETase

Tags

6xHis Tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-Bsu

Plasmid

#163911

Purpose

Expresses Bsu large fragment for affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

Bsu polymerase

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-Cas9-His

Plasmid

#98158

Purpose

Purification of Cas9 protein

Depositor

Caixia Gao

Article

Liang et al Nat Commun. 2017 Jan 18;8:14261. doi: 10

Insert

NLS-Cas9-NLS

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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hPU.1ΔN165-pET28b

Plasmid

#199796

Purpose

Expressed human PU.1/Spi-1 ETS domain (residues 165 to 270) in E. coli

Depositor

Gregory Poon

Article

Xhani et al Sci Adv. 2020 Feb 21;6(8):eaay3178. doi:

Insert

spi1 (SPI1 Human)

Tags

None

Expression

Bacterial

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET29b_1_anti-GFP-nanobody

Plasmid

#210394

Purpose

Express anti-GFP nanobody in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

1_anti-GFP-nanobody

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pet28a-His6-Keap1

Plasmid

#62454

Purpose

His6 tagged Keap1 for E.coli expression

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Tag

Expression

Bacterial

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-Ec.coaBC (pCLK1210)

Plasmid

#50448

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylase

Depositor

Tadhg Begley, Erick Strauss

Article

Strauss et al J Biol Chem. 2001 Apr 27;276(17):13513-6

Insert

PPCS PPCDC

Tags

6XHIS tag

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2014

Availability

Academic Institutions and Nonprofits only

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pET21d-myc-BirA

Plasmid

#109424

Purpose

Bacterial expression of biotin ligase (BirA) with an N-terminal myc epitope

Depositor

Martin Spiess

Article

Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

BirA (birA )

Tags

myc

Expression

Bacterial

Promoter

T7

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pET28-his-3C_Tau352

Plasmid

#100092

Purpose

Expresses human full-length fetal tau protein with the N-terminal 3C-cleavable His6 tag

Depositor

Nikolai Sluchanko

Article

Tugaeva et al PLoS One. 2017 Jun 2;12(6):e0178933. doi

Insert

Fetal microtubule-associated protein tau (MAPT Human)

Tags

His6

Expression

Bacterial

Mutation

wild type sequence

Promoter

T7

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-nanoHER2-SpyTag003

Plasmid

#216312

Purpose

Expresses nanoHER2-SpyTag003 in bacterial cells. nanoHER2 is an anti-HER2 nanobody.

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

nanoHER2-SpyTag003

Tags

His6 tag and SpyTag003

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28b-zDjCas13d-His

Plasmid

#234481

Purpose

Plasmid for bacterial expression and purification of DjCas13d protein (zebrafish codon-optimized)

Depositor

Miguel Angel Moreno-Mateos

Article

Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

Insert

DjCas13d

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b-AK1-His

Plasmid

#124135

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Adenylate kinase (AK1 Chicken)

Tags

6x-His

Expression

Bacterial

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG94-15

Plasmid

#228971

Purpose

For bacterial expression of anti-GFP nanobody LaG94-15, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-15

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET22b-CPDBamHI-Leu

Plasmid

#38256

Depositor

Matthew Bogyo, Aimee Shen

Article

Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

Type

Empty backbone

Tags

6xHis and Vibrio cholerae MARTX toxin cysteine pr…

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only

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