We narrowed to 4,324 results for: Pet2;

1. pET28-eIF4E-TwStrep

   Plasmid

   #208638

   Purpose

   for E. coli expression of C-ter Twin Strep tagged human eIF4E (ENST00000450253)

   Depositor

   Quanfu Ma

   Insert

   human eIF4E (ENST00000450253) (EIF4E Human)
   

   Tags

   6xHis and Twin Strep

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21a-LysRS-His

   Plasmid

   #124114

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Lysine-tRNA ligase (lysU Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a SnoopTag-MBP

   Plasmid

   #72323

   Purpose

   Bacterial expression of Maltose Binding Protein linked to SnoopTag, a peptide tag forming a spontaneous covalent bond with its protein partner Snoopcatcher.

   Depositor

   Mark Howarth

   Article

   Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

   Insert

   pET28a SnoopTag-MBP
   

   Tags

   N-terminal His6 tag. MBP is fused at the C-termin…

   Expression

   Bacterial

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a T7pCONS sfGFP

   Plasmid

   #154462

   Purpose

   pET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP

   Depositor

   Daniel Daley

   Article

   Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

   Insert

   super folder GFP
   

   Tags

   His-thrombin

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21a-AspRS-His

   Plasmid

   #124106

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Aspartate-tRNA ligase (aspS Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21a-ProRS-His

   Plasmid

   #124117

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Prolyl-tRNA ligase (proS Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21a-TyrRS-His

   Plasmid

   #124121

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Tyrosyl-tRNA ligase (tyrS Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21a-ValRS-His

   Plasmid

   #124122

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Takuya Ueda, Sebastian Maerkl

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Valyl-tRNA ligase (valS Escherichia coli)
   

   Tags

   6x-His

   Expression

   Bacterial

   Available Since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET246-pUC57 12xMS2V6

   Plasmid

   #104390

   Purpose

   MS2V6 cloning

   Depositor

   Robert Singer, Evelina Tutucci

   Article

   Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

   Insert

   12xMS2V6
   

   Expression

   Bacterial

   Mutation

   All Loops are U-variants, interspaced by 50 nucle…

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21a-GluRS-His

    Plasmid

    #124109

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Glutamyl-tRNA ligase (gltX Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZH82 pET28a-Rai1

    Plasmid

    #231651

    Purpose

    Bacterial expression vector expressing yeast S. cerevisiae rai1, no tag

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    rai1 (RAI1 Budding Yeast)
    

    Expression

    Bacterial

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-mH6-Cas12c1

    Plasmid

    #120872

    Purpose

    Expresses E. coli codon optimized Cas12c1 effector in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Cas12c1
    

    Use

    CRISPR

    Tags

    mH6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    lac

    Available Since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21-pelB-LaG-16

    Plasmid

    #172746

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-16
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. UbcH5c S22R pET28a

    Plasmid

    #12644

    Depositor

    Rachel Klevit

    Article

    Brzovic et al Mol Cell. 2006 Mar 17. 21(6):873-80.

    Insert

    Ubch5c - Ubiquitin conjugating enzyme (UBE2D3 Human)
    

    Expression

    Bacterial

    Available Since

    July 31, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pET21 gamma-tubulin

    Plasmid

    #101825

    Purpose

    production of gamma-tubulin in bacteria

    Depositor

    Ana Carrera, Maria Alvarado-Kristensson

    Article

    Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

    Insert

    gamma-tubulin 1 (TUBG1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Whole protein

    Promoter

    T7

    Available Since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-mH6-Cas12i1

    Plasmid

    #120882

    Purpose

    Expresses E. coli codon optimized Cas12i1 effector in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Cas12i1
    

    Use

    CRISPR

    Tags

    mH6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    lac

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28b-SpRY-His

    Plasmid

    #179320

    Purpose

    Plasmid for bacterial expression and purification of SpRY-Cas9

    Depositor

    Julián Cerón, Miguel Angel Moreno-Mateos

    Article

    Vicencio et al Nat Commun. 2022 May 12;13(1):2601. doi:

    Insert

    Human codon-optimized Cas9 variant SpRY
    

    Use

    CRISPR

    Tags

    6xHis and SV40-NLS

    Expression

    Bacterial

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/ N13…

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21a-SerRS-His

    Plasmid

    #124118

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Seryl-tRNA ligase (serS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET21a-GlnRS-His

    Plasmid

    #124108

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Glutaminyl-tRNA ligase (glnS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-AviTag-SnoopLigase

    Plasmid

    #105626

    Purpose

    Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with AviTag for biotinylation

    Depositor

    Mark Howarth

    Article

    Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

    Insert

    AviTag-SnoopLigase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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