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Plasmid#235633PurposeEncodes V5-GFP with an N-terminal nuclear export sequence and a C-terminal FLAG-TEV cleavage site-AviTag. Used to express a control substrate for ALIBi.DepositorInsertGFP
UseTagsFLAG-TEV cleavage site-AviTag and NES-V5ExpressionMammalianMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRB133.2
Plasmid#181950PurposeaTc-inducible expression of PhoP with C-terminal mNeonGreen fusion. Also contains mCherry under PhoP-controlled promoter PvirKDepositorInsertPhoP-Salmonella enterica subsp. enterica serovar Typhimurium (AX04_RS23485 Synthetic, PhoP-Salmonella enterica subsp. enterica serovar Typhimurium)
UseTagsmNeonGreenExpressionBacterialMutationPromoterPhoP-mNG-PLtetO-1; mCherry-PvirK; tetR-J23106Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Sig10(M1)-Fc-IG
Plasmid#183723PurposeExpresses the fusion protein containing the extracellular part of the human Siglec-10 R47Q mutant and the Fc region of human IgG1, and GFPDepositorInsertSiglec-10
UseRetroviralTagsFc of human IgG1ExpressionMutationR47QPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Sig10(M2)-Fc-IG
Plasmid#183724PurposeExpresses the fusion protein containing the extracellular part of the human Siglec-10 A108V mutant and the Fc region of human IgG1, and GFPDepositorInsertSiglec-10
UseRetroviralTagsFc of human IgG1ExpressionMutationA108VPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Sig10(DM)-Fc-IG
Plasmid#183725PurposeExpresses the fusion protein containing the extracellular part of the human Siglec-10 R47Q A108V mutant and the Fc region of human IgG1, and GFPDepositorInsertSiglec-10
UseRetroviralTagsFc of human IgG1ExpressionMutationR47Q and A108VPromoterAvailable sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
KG#797
Plasmid#110916PurposeExpresses the active zone - enriched protein Sentryn-GFP in a subset of cholinergic ventral nerve cord motor neuronsDepositorInsertsunc-129 promoter
STRN-1 (Sentryn)-GFP
unc-54 3' control region with 1 intron just upstream and 1 intron in control region
UseTagsGFP (contains 3 artificial introns)ExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNG1304
Plasmid#162488PurposeExpresses HelD K239A from Bacillus subtillisDepositorInsertHelD (helD Bacillus subtillis 168)
UseTags6His-V5ExpressionBacterialMutationChanged Lysine 239 to Alanine (K239A)PromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCR4-*mRFP
Plasmid#47118PurposeCan be used to make an RNA probe for whole-mount in situ hybridization, for in-frame fusion proteins or as a subcloning step for mRFP. Note that the coding sequence for mRFP lacks the start codon.DepositorInsert*mRFP
UseTagsExpressionBacterialMutationNo start codonPromoterLacAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
Cherry-Fibrillarin-C1
Plasmid#134539PurposeExpresses Cherry-Fibrillarin fusion proteinDepositorInsertFibrillarin (FBL Human)
UseTagsCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Cdc42-T17N
Plasmid#12976DepositorInsertCdc42 DN (CDC42 Human)
UseTagsEGFPExpressionMammalianMutationT17N Dominant negativePromoterCMVAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLX307-CRBN-3F-BirA
Plasmid#220146PurposeExpresses CRBN-BirA for CRBN substrate identification by E-STUBDepositorInsertCereblon (CRBN Human)
UseLentiviralTags3F-BirAExpressionMammalianMutationPromoterEF-1aAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-RIα-EGFP
Plasmid#181840PurposeGFP-tagged PKA RIα subunit for mammalian expression.DepositorInsertRIα-EGFP (PRKAR1A Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-CCO-IRES-Puromycin
Plasmid#134861PurposeExpresses EGFP-tagged inner mitochondrial membrane markerDepositorInsertcytochrome c oxidase subunit VIII (COX8A Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pECE HA AMPKa1 WT
Plasmid#69504PurposeExpresses AMPK alpha1 in mammalian cellsDepositorInsert5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterSV40 early promoterAvailable sinceApril 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSKC12 - DelQ
Plasmid#110493PurposeMonitor the expression of Luciferase driven by a mutated NRAS 5'-UTR.DepositorInsertNRAS 5'- DelQ (bp 30-254) (NRAS Human)
UseLuciferaseTagsFirefly luciferaseExpressionMutationDeletion of the first 29 base pairs of the NRAS 5…PromoterT7Available sinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Synaptophysin-TdTomato-IRES2-OHT-Cre
Plasmid#134308PurposeExpresses Synaptophysin-TdTomato and also tamoxifen dependant Cre (OHT-Cre, aka Cre-ER)DepositorInsertsUseTagsTdTomatoExpressionMammalianMutationPromoterCAGAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM354 pET28a-GGG-Titin[V15P]-23-K-19-C-15
Plasmid#226350PurposeExpression of proteasome substrate with tail for molecular rulerDepositorInserttitin (TTN Human)
UseTagsExpressionBacterialMutationV15PPromoterAvailable sinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-PKAcat-mCherry
Plasmid#181841PurposemCherry-tagged PKA catalytic subunit for mammalian expression.DepositorInsertPKAcat-mCherry (PRKACA Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMito-LSSmKate1-N1
Plasmid#31878DepositorInserthuman cytochrome C oxidase subunit VIII (COX8A Human)
UseTagsExpressionMammalianMutationK69Y/P131T/S148G/M167E/T183S/M196V compared to mK…PromoterAvailable sinceSept. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pECE-M2-PPP1R12C wt
Plasmid#31659DepositorInsertProtein Phosphatase 1 Regulatory Subunit 12C (PPP1R12C Human)
UseTagsM2ExpressionMammalianMutationcodon-optimizedPromoterAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only