We narrowed to 11,803 results for: nsf

1. pAAV-VTKD3

   Plasmid

   #170848

   Purpose

   AAV backbone for Flp-dependent transgene expression of any transgene in cortical interneurons under the control of the Dlx enhancer.

   Depositor

   Jordane Dimidschstein

   Article

   Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-VTKD4

   Plasmid

   #170849

   Purpose

   AAV backbone for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of any transgene in cortical interneurons under the control of the Dlx enhancer.

   Depositor

   Jordane Dimidschstein

   Article

   Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-iLMO4 (GLucM23-iChloC-EYFP)

   Plasmid

   #114102

   Purpose

   fusion protein of Gaussia luciferase variant M23, improved slowChloC anion conducting ChR, and enhanced yellow fluorescent protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Park et al J Neurosci Res. 2017 Sep 1. doi: 10.1002

   Insert

   GLucM23-iChloC-EYFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXS-IRES-Blast SHMT2res

   Plasmid

   #106301

   Purpose

   Expresses SHMT2

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   serine hydroxymethyltransferase 2 (SHMT2 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Silent mutations destroy sgRNA targeting sites

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEMS2157

   Plasmid

   #70118

   Purpose

   Promoterless AAV plasmid with MCS to insert promoter for expression of EmGFP. Contains WPRE.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-MCS-EmGFP-WPRE
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-mCherry.3xFLAG.NOS1APΔC20-WPRE

   Plasmid

   #127865

   Purpose

   pAAV plasmid expressing an mCherry.3xFLAG.NOS1APΔC20 fusion protein under the hSyn promoter

   Depositor

   Florian Freudenberg

   Article

   Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

   Insert

   Nos1ap (Nos1ap Mouse)
   

   Use

   AAV

   Tags

   3xFLAG and mCherry

   Mutation

   deleted the c-terminal 20 amino acids

   Promoter

   hSyn

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2+ cMyc Dam_f_GFPoNLS

   Plasmid

   #85820

   Purpose

   iDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

   Depositor

   Joachim Wittbrodt

   Article

   Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

   Insert

   cMyc Dam_f_GFPoNLS
   

   Tags

   cMyc and oNLS (optimized nuclear localization sig…

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   L122A.

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. KO101: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-Dnmt3a3L

   Plasmid

   #121833

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   x-dCas9(3.7)/Dnmt3a-3L (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-FLEX-tmeGFP-IRES-nls-mRuby2

   Plasmid

   #63060

   Purpose

   Expresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

   Depositor

   Bernardo Sabatini

   Article

   Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

   Insert

   nls-mRuby2
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-miRFP2

    Plasmid

    #178968

    Purpose

    Expresses miRFP2 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    miRFP2
    

    Use

    AAV

    Tags

    no

    Expression

    Mammalian

    Mutation

    NO

    Promoter

    CAG

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-C-moxGFP-Puro-H2BC11-MMEJ

    Plasmid

    #207760

    Purpose

    MMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pAAV-hSyn-mCherry.3xFLAG.NOS1APC20-WPRE

    Plasmid

    #127866

    Purpose

    pAAV plasmid expressing an mCherry.3xFLAG.NOS1APC20 fusion protein under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

    Insert

    Nos1ap (Nos1ap Mouse)
    

    Use

    AAV

    Tags

    3xFLAG and mCherry

    Mutation

    C-terminal 20 amino acids only

    Promoter

    hSyn

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. SIN-PGK-Cas9-V5-WPRE

    Plasmid

    #87876

    Purpose

    To produce lentiviral vector for Cas9 editing

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    spCas9
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    Human codon-optimized

    Promoter

    mouse PGK

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEMS1980

    Plasmid

    #49111

    Purpose

    Promoterless AAV plasmid with MCS to insert promoter for expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-MCS-iCre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pAAV-FLEXfrt-SaCas9-U6-sgGabrg1

    Plasmid

    #124870

    Purpose

    Mutagenesis of Gabrg1

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gabrg1 (Gabrg1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. p1193-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS-3XmiR122BS

    Plasmid

    #129531

    Purpose

    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme with 3x miR-122 binding sites in 3' UTR

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Inserts

    codon-optimized AcrIIC3Nme
    sgRNA_Rosa26
    

    Use

    AAV

    Tags

    FLAG/NLS

    Promoter

    U6 and cytomegalovirus-enhancer chicken β-actin p…

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEF1α-M2rtTA-T2A-Puro

    Plasmid

    #169175

    Purpose

    piggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174

    Depositor

    Igor Slukvin

    Article

    Jung et al Cell Rep. 2021 Feb 16;34(7):108758. doi:

    Insert

    M2rtTA-T2A-Puro
    

    Use

    Piggybac transposon

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Promoter

    EF1a

    Available Since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEMS2044

    Plasmid

    #79652

    Purpose

    ssAAV genome with Ple255 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    ssAAV Ple255-EmGFP WPRE
    

    Use

    AAV

    Promoter

    PAX6 HS234Z Enhancer

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-DYRK2-2A-GFP

    Plasmid

    #118284

    Purpose

    Expresses mouse DYRK2 tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.

    Depositor

    Vance Lemmon

    Article

    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

    Insert

    dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 (Dyrk2 Mouse)
    

    Use

    AAV

    Tags

    2A peptide and GFP

    Expression

    Mammalian

    Promoter

    CMV with beta global intron

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGW045 AAV-CRISPRa base vector pAAV_OE-U6sgbbSapI(MS2)-EF1a-MPH-WPRE

    Plasmid

    #192161

    Purpose

    AAV-CRISPRa base vector

    Depositor

    Sidi Chen

    Article

    Wang et al Nat Immunol. 2019 Nov;20(11):1494-1505.

    Type

    Empty backbone

    Use

    AAV

    Mutation

    NA

    Available Since

    Oct. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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