We narrowed to 21,826 results for: Sis

1. pCS020

   Plasmid

   #207915

   Purpose

   Level 0 for gRNA in D3 module (5' TCCC-3' CTGC)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU6-AarI-scaffold-term
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS017

   Plasmid

   #207909

   Purpose

   Level 0 for gRNA in C1 module (5' GGCT-3' AGCC)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU6-AarI-scaffold-term
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKE012

   Plasmid

   #207914

   Purpose

   Level 0 for gRNA in AD2 module (5' TGAC-3' TCCC)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU3-AarI-scaffold-term
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKE010

   Plasmid

   #207910

   Purpose

   Level 0 for gRNA in C2 module (5' AGCC-3' TTCG)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU3-AarI-scaffold-term
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCS019

   Plasmid

   #207913

   Purpose

   Level 0 for gRNA in D1 module (5' TCAG-3' TGAC)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU6-AarI-scaffold-term
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Dox-SOScat

   Plasmid

   #207329

   Purpose

   PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized catalytic domain of SOS (SOScat)

   Depositor

   Jared Toettcher

   Article

   Underhill et al Development. 2023 Aug 15;150(16):dev2016

   Insert

   son of sevenless homolog 2 variant, catalytic domain
   

   Use

   Tags

   myristoylation tag - BFP

   Expression

   Bacterial

   Mutation

   range: 593 to 1078

   Promoter

   Tet Response Element

   Available since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-C1-hEFA6C

   Plasmid

   #206414

   Purpose

   Mammalian expression of hEFA6C

   Depositor

   Kazuhisa Nakayama, Hye-Won Shin

   Article

   Ueda et al FEBS Lett. 2013 Jun 5;587(11):1617-23. d

   Insert

   EFA6C (PSD2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZCS41 (U6p::GCGAAGTGACGGTAGACCGT)

   Plasmid

   #193050

   Purpose

   Encodes guide RNA expression targeting PX740 landing pad

   Depositor

   Patrick Phillips

   Article

   Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

   Insert

   Guide RNA
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 NKX6-2-Venus S246D

   Plasmid

   #203716

   Purpose

   human NKX6-2 S246D phosphomimic mutant

   Depositor

   Federico Herrera

   Article

   Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

   Insert

   NKX6-2 (S246D) fused with Venus (NKX6-2 Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   NKX6-2 (S246D)

   Promoter

   CMV

   Available since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1 NKX6-2-Venus T252D

    Plasmid

    #203718

    Purpose

    human NKX6-2 T252D phosphomimic mutant

    Depositor

    Federico Herrera

    Article

    Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

    Insert

    NKX6-2 (T252D) fused with Venus (NKX6-2 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    NKX6-2 (S252D)

    Promoter

    CMV

    Available since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 NKX6-2-Venus

    Plasmid

    #203712

    Purpose

    Constitutive mammaliam expression of human NKX6-2 cDNA fused with the Venus fluorescent protein

    Depositor

    Federico Herrera

    Article

    Ferreira-Peralta et al Biochim Biophys Acta Mol Basis Dis. 2023

    Insert

    NKX6-2 fused with Venus (NKX6-2 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTet-Bxb1-Xis-mScarlet

    Plasmid

    #195570

    Purpose

    Expresses Bxb1 excisionase fused with mScarlet for fluorescence

    Depositor

    Richard Murray

    Article

    Pandey et al ACS Synth Biol. 2023 Feb 17;12(2):511-52

    Insert

    Bxb1 Excisionase Fused With mScarlet
    

    Use

    Synthetic Biology

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    P7

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. NK809

    Plasmid

    #196406

    Purpose

    Strong expression of firefly and nanoluc luciferases codon-optimized for S. rosetta

    Depositor

    Nicole King

    Article

    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

    Insert

    Sro5EFL-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    N/A (synthetic)

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. NK810

    Plasmid

    #196407

    Purpose

    Nanoluc luciferase under S. rosetta SPAG6 promoter with internal actin-firefly control

    Depositor

    Nicole King

    Article

    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

    Insert

    Sro5spag6-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    N/A (synthetic)

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. NK811

    Plasmid

    #196408

    Purpose

    Nanoluc luciferase under S. rosetta SPAG6 promoter with mutated RFX binding site with internal actin-firefly control

    Depositor

    Nicole King

    Article

    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

    Insert

    Sro5spag6_mut-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    N/A (synthetic)

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCA528 AMSHLP 16-218

    Plasmid

    #180609

    Purpose

    Bacterial expression for AMSHLP MIT domain residues 16-218. Internal ID: WISP20-83

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    AMSHLP residues 16-218
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 16-218

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. TEG +Q111T

    Plasmid

    #187435

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1
    ATP1B1
    

    Use

    Tags

    Expression

    Insect

    Mutation

    changed glutamine at 117 to threonine

    Promoter

    PH and p10

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSF-NgPglO

    Plasmid

    #198204

    Purpose

    pSN18 derivative encoding N. gonorrhoeae PglO with C-terminal FLAG epitope tag

    Depositor

    Michael Jewett

    Article

    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

    Insert

    NgPglO
    

    Use

    Tags

    Flag tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJL1-AcrA

    Plasmid

    #198214

    Purpose

    pJL1 plasmid encoding wildtype AcrA from C. jejuni containing 2 native internal glycosylation sites recognized by PglB and a C-terminal 6x-His tag

    Depositor

    Michael Jewett

    Article

    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

    Insert

    AcrA
    

    Use

    Tags

    6x His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOBD2

    Plasmid

    #196826

    Purpose

    Yeast two-hybrid ; Gal4 DNA binding domain

    Depositor

    Stan Fields

    Article

    Uetz et al Nature. 2000 Feb 10;403(6770):623-7. doi

    Type

    Empty backbone

    Use

    Tags

    Gal4 DNA binding domain

    Expression

    Yeast

    Mutation

    Promoter

    ADH

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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