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pCMV-deltaR8.2(A14C,E45C,A92E)

Plasmid

#79048

Purpose

mammalian expression, lentiviral packaging

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pIRES.Puro.EGFP

Plasmid

#24176

Depositor

Robert Sobol

Article

Jelezcova et al Mutat Res. 2010 Apr 1;686(1-2):57-67. do

Insert

EGFP

Expression

Mammalian

Mutation

x

Available Since

April 9, 2010

Availability

Academic Institutions and Nonprofits only
pFastBac GST-Plk4

Plasmid

#80264

Purpose

baculovirus creation plasmid for GST-Plk4

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

Plk4 (PLK4 Human)

Tags

GST

Expression

Insect

Available Since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pCR1003

Plasmid

#71821

Purpose

Express Streptococcus pyogenes dCas9 (D10A, H840A)

Depositor

Jacob Corn

Article

Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

Insert

T7pr_10xHis-MBP-TEV-SPydCas9 (D10A, H840A)

Use

CRISPR

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA-FRT-FAK0

Plasmid

#45047

Purpose

FAK fret sensor without helix

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pFUS_A

Plasmid

#31028

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

LacZ + BsaI restriction sites

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pRS406-PHO5-GFP-2xOsh2 PH domain [short]

Plasmid

#58829

Purpose

Expresses GFP-Tagged Osh2 PH domain in yeast

Depositor

Tim Levine

Article

Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

Insert

2xOsh2 PH domain [short] (OSH2 Budding Yeast)

Tags

GFP and Myc

Expression

Yeast

Promoter

PHO5

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pQF-mNGsacB

Plasmid

#203673

Purpose

pQF with mNeonGreen and sacB under control of PQ5

Depositor

Urs Jenal

Article

Klotz et al Appl Environ Microbiol. 2023 May 18:e002

Inserts

mNeonGreen
sacB

Expression

Bacterial

Mutation

Has synonymous (silent) mutations to remove BsrGI…

Promoter

PQ5

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pMLC-BB1

Plasmid

#166922

Purpose

Backbone for assembly of multi-level gene expression controllers

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

CyOFP - Orange fluorescent protein - flanked by BsaI sites

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23100 constitutive

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pJB089

Plasmid

#98914

Purpose

Express Dronpa tagged ZapA and pAmCherry1 tagged FtsZ in E coli

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Inserts

FtsZ
ZapA

Tags

Dronpa and pAmCherry1

Expression

Bacterial

Promoter

T5-lac

Available Since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pRetroSuper-GFP TIF1gamma

Plasmid

#15721

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

TIF1 gamma shRNA (TRIM33 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pCR1055

Plasmid

#71823

Purpose

Express Streptococcus pyogenes nCas9 (H840A) nickase carrying two C-terminal SV40 NLS

Depositor

Jacob Corn

Article

Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

Insert

T7pr_6xHis-MBP-TEV-SPynCas9 (H840A)-2xNLS

Use

CRISPR

Available Since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pTHS

Plasmid

#166938

Purpose

Toehold switch multi-level gene expression controller (MLC)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Genetic circuit expressing GFP- Toehold switch

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTAC

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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CMVTCRa_candidate5

Plasmid

#164999

Purpose

Expression of candidate TCR #5 TCRalpha cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRAV (TRA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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CMVTCRb_candidate5

Plasmid

#165000

Purpose

Expression of candidate TCR #5 TCRbeta cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRB (TRB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pJY3948

Plasmid

#113338

Purpose

6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expression

Depositor

Suzanne Sandmeyer

Article

Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

Insert

2-pyrone synthase, codon optimized for Y. lipolytica

Tags

6XHis

Expression

Yeast

Mutation

Please see depositor comments

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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GGE038

Plasmid

#120785

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by NotI restriction enzyme after the assembly

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

InsDown_Zeta-NotI

Use

Cloning/assembly plasmid

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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GGE067

Plasmid

#120784

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by NotI restriction enzyme after the assembly

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

InsUp_Zeta-NotI

Use

Cloning/assembly plasmid

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLX304 LacZ-V5 blast

Plasmid

#83099

Purpose

Lentiviral vector for constitutive expression of LacZ with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

LacZ

Use

Lentiviral

Tags

2x V5 tag

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pCfB2190

Plasmid

#67535

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae XI-2 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Expression

Yeast

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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