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pCL0918

Plasmid

#252411

Purpose

Mitochondria-tethered construct for the RAPID-Release approach

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

Histone H4 (HIST1H4F Human)

Tags

EGFP-TVMVx2-FRB-OMP25

Expression

Mammalian

Mutation

Truncation: aa 1-46 only

Promoter

CMV

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pCL0946

Plasmid

#252416

Purpose

Mitochondria-tethered construct for the RAPID-Release approach

Depositor

Andrew Bowman

Article

Apta-Smith et al EMBO J. 2018 Oct 1;37(19):e98714. doi: 1

Insert

Histone H4 (HIST1H4F Human)

Tags

EGFP-TVMVx2-FRB-OMP25

Expression

Mammalian

Mutation

Truncation: aa 1-20 only

Promoter

CMV

Available Since

March 10, 2026

Availability

Academic Institutions and Nonprofits only

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pKG0893_pHarbor-LentiX1_backbone

Plasmid

#239704

Purpose

PaqCI Golden Gate assembly plasmid encoding the LentiX1 backbone

Depositor

Kate Galloway

Article

Peterman et al Nucleic Acids Research, Volume 53, Issue

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pYPQ166-TREX2-N

Plasmid

#244022

Purpose

Gateway entry plasmid (attL1& attR5) expressing TREX2 exonuclease fused to the N-terminus of zSpCas9, connected by a flexible XTEN linker, without promoter

Depositor

Yiping Qi

Article

Wang et al Plant Physiol. 2025 Oct 31;199(3):kiaf50

Insert

Cas9

Use

CRISPR

Tags

TREX2

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ166-AtEXO1B-N

Plasmid

#244023

Purpose

Gateway entry plasmid (attL1& attR5) expressing AtEXO1B exonuclease fused to the N-terminus of zSpCas9, connected by a flexible XTEN linker, without promoter

Depositor

Yiping Qi

Article

Wang et al Plant Physiol. 2025 Oct 31;199(3):kiaf50

Insert

Cas9

Use

CRISPR

Tags

AtEXO1B

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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AGG1984 UbL40-Bs

Plasmid

#242439

Purpose

Ae aegypti UbL40 promoter expressing barstar

Depositor

Luke Alphey

Article

Nevard et al Commun Biol. 2025 Aug 4;8(1):1154. doi:

Insert

Barstar

Expression

Insect

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAttBF3Neo

Plasmid

#244886

Purpose

Neo resistant attB phiC31 recombination-mediation insertion (RMI) vector for C. elegans

Depositor

Michael Nonet

Article

Beck et al MicroPubl Biol. 2025 Sep 2;2025:10.17912

Type

Empty backbone

Use

Phic31 recombinase mediated insertion vector

Expression

Worm

Promoter

none

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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Strep-Halo

Plasmid

#219682

Purpose

Mammalian expression of Strep-HaloTag fusion protein

Depositor

Alison Twelvetrees

Article

Smith et al iScience. 2026 Feb 2;29(3):114875. doi:

Insert

StrepTag-HaloTag

Expression

Mammalian

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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NFfshb-t2a-GFP

Plasmid

#194356

Purpose

over expression vector for killifish fshb tagged with GFP

Depositor

Itamar Harel

Article

Moses et al eLife. 2023 Oct 24;12:e85960. doi: 10.75

Insert

follicle stimulating hormone beta subunit

Use

Lentiviral

Tags

t2a-GFP

Expression

Mammalian

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Tol2-TLCV2

Plasmid

#196331

Purpose

the TLCV2 plasmid with Tol2 recombination sites for integration

Depositor

Itamar Harel

Article

Moses et al eLife. 2023 Oct 24;12:e85960. doi: 10.75

Insert

Cas9-2A-eGFP

Use

CRISPR; Fish expression

Expression

Bacterial, Mammalian, and Y…

Promoter

Tight TRE promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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JD134

Plasmid

#229490

Purpose

Adenoviral E4 gene

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

E4

Use

AAV and Adenoviral

Expression

Mammalian

Promoter

wt promoter

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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SJZ6-HIS5

Plasmid

#228259

Purpose

Template plasmid for yeast gene knockout, uses auxotrophic marker his5 (Schizosaccharomyces Pombe) (complements S.c. his3)

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

his5

Expression

Yeast

Promoter

TEF1 S.c.

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGLU_103

Plasmid

#225785

Purpose

This is the promoter region taken from upstream of Tn5 transposase in pBAM1. It can be considered the "default" promoter to use.

Depositor

Nili Ostrov

Article

Gilbert et al bioRxiv 2025.12.22.696024

Insert

Ptn5_[Tn5]

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGLU_85

Plasmid

#225800

Purpose

This is the promoter region taken from upstream of Himar transposase in pMarC9-Kan. It can be considered the "default" promoter to use.

Depositor

Nili Ostrov

Article

Gilbert et al bioRxiv 2025.12.22.696024

Insert

PmarC9_[Himar]

Use

Synthetic Biology

Mutation

N/A

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAM292

Plasmid

#226129

Purpose

Expression of His6-SUMO-Ub-mEos3.2-intein-CBD

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

His6-SUMO-Ub-mEos3.2-intein-CBD

Expression

Bacterial

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM296

Plasmid

#226132

Purpose

Expression of Ufd1L55R-3C-His6

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

Ufd1L55R-3C-His6

Expression

Bacterial

Mutation

L55R

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM295

Plasmid

#226131

Purpose

Expression of Ufd1R64C-3C-His6

Depositor

Andreas Martin

Article

Williams et al Mol Cell. 2023 Mar 2;83(5):759-769.e7. d

Insert

Ufd1R64C-3C-His6

Expression

Bacterial

Mutation

R64C

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pSpy

Plasmid

#220184

Purpose

sgRNA expression vector - promoterless backbone

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

promoterless backbone

Use

CRISPR

Expression

Bacterial

Promoter

promoterless

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyA

Plasmid

#220185

Purpose

sgRNA expression vector - pEM7 promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pEM7 promoter

Use

CRISPR

Expression

Bacterial

Promoter

pEM7

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyC

Plasmid

#220187

Purpose

sgRNA expression vector - pBG42 promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG42 promoter

Use

CRISPR

Expression

Bacterial

Promoter

pBG42

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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