We narrowed to 63,782 results for: cell

1. pMRX-IP-GFP-LC3-mRuby3

   Plasmid

   #184898

   Purpose

   Stable expression of GFP-LC3-mRuby3 in mammalian cells to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Okawa et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   LC3B
   

   Tags

   EGFP and mRuby3

   Expression

   Mammalian

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCVpuro-T7-hAIM2-EGFP

   Plasmid

   #51608

   Purpose

   Express T7 and GFP-tagged hAIM2 in mammalian cells

   Depositor

   Emad Alnemri

   Article

   Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

   Insert

   AIM2 (AIM2 Human)
   

   Use

   Retroviral

   Tags

   EGFP and T7

   Expression

   Mammalian

   Promoter

   MESV

   Available Since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX-uORF-HA-birA*-STOP-IRES-Puro

   Plasmid

   #120558

   Purpose

   Expresses control HA-birA* in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   birA
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   R118G

   Promoter

   CMV

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS SK mCherryROSAbsr

   Plasmid

   #54321

   Purpose

   Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.

   Depositor

   Silvestro Conticello

   Article

   Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

   Insert

   mCherry and BlasticidinS-resistance
   

   Use

   CRISPR, Mouse Targeting, and TALEN

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tet-FRNK-GFP

   Plasmid

   #83472

   Purpose

   Lentiviral vector for doxycycline-inducible expression of FRNK in mammalian cells

   Depositor

   Barry Gumbiner

   Article

   Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

   Insert

   FRNK (PTK2 Chicken)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   FRNK is the non-catalytic c-term domain of FAK ki…

   Promoter

   doxycycline-inducible promoter

   Available Since

   Oct. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Beclin-HA S-D

   Plasmid

   #46995

   Purpose

   Expresses Beclin1 in mammalian cells

   Depositor

   Kunliang Guan

   Article

   Russell et al Nat Cell Biol. 2013 Jul;15(7):741-50. do

   Insert

   becn1
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S14D

   Available Since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

7. Nano-lantern-mitochondria/pcDNA3

   Plasmid

   #51973

   Purpose

   mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to mitochondria

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern
   

   Tags

   CoxVIIIx2

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Available Since

   March 28, 2014

   Availability

   Academic Institutions and Nonprofits only

8. rat APOBEC1

   Plasmid

   #112858

   Purpose

   plasmid for expression of rat APOBEC1 in mammalian cells

   Depositor

   Silvestro Conticello

   Article

   Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108

   Insert

   APOBEC1 (Apobec1 Rat)
   

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Oct. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMRXIP-Myc-YKT6

   Plasmid

   #116945

   Purpose

   Expressing Myc-YKT6 for mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Matsui et al J Cell Biol. 2018 Aug 6;217(8):2633-2645

   Insert

   Synaptobrevin homolog YKT6 (YKT6 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lmajor_gp63_10_0460_E265A

    Plasmid

    #171641

    Purpose

    Expression of catalytically inactive L. major glcyoprotein-63 (E265A) from chromosome 10 in mammalian cells

    Depositor

    Dennis Ko

    Article

    Antonia et al Front Cell Infect Microbiol. 2019 Aug 7;

    Insert

    LmjF.10.0460 E265A
    

    Tags

    Myc-His

    Expression

    Mammalian

    Mutation

    E265A

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. RCAS-myc

    Plasmid

    #11548

    Depositor

    Sandra Orsulic

    Article

    Orsulic et al Cancer Cell. 2002 Feb . 1(1):53-62.

    Insert

    c-myc (MYC Human)
    

    Use

    Retroviral

    Available Since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pAcGFP1-N1-RvLEAM

    Plasmid

    #90035

    Purpose

    To express GFP-tagged RvLEAM in mammalian cells transiently

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

    Insert

    RvLEAM
    

    Tags

    AcGFP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6-mir144 EF1Alpha-puro-T2A-BFP

    Plasmid

    #164791

    Purpose

    Express miR-144 under the U6 promoter in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR-144 (MIR144 Human)
    puro-T2A-BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1Alpha and U6

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. GL2 shRNA

    Plasmid

    #86083

    Purpose

    A lentiviral construct for building stable cell lines expressing GL2 shRNA via puromycin selection.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    control shRNA
    

    Promoter

    U6

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. EK0302 SFFV-mCherry-SG(Bdnf.s2)SG-EGFP (FLP-IN)

    Plasmid

    #191179

    Purpose

    Negative control sensor in human cells.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:Bdnf.s2:EGFP (negative control)
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. JP1520 PIK3CA H1047R HA

    Plasmid

    #14572

    Depositor

    Joan Brugge

    Article

    Isakoff et al Cancer Res. 2005 Dec 1. 65(23):10992-100

    Insert

    PIK3CA H1047R (PIK3CA Bovine)
    

    Use

    Retroviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    H1047R

    Available Since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pDEST-CMV 3xFLAG-LC3B2-GFP

    Plasmid

    #123109

    Purpose

    Expresses 3xFLAG-LC3B2-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3B2

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)
    

    Tags

    3xFLAG and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHTN HaloTag CMV-neo/ERGIC2-variant

    Plasmid

    #51477

    Purpose

    Expresses ERGIC2-variant in mammalian cells, HaloTag for cellular localization

    Depositor

    Simon Kwok

    Article

    Kwok et al DNA Cell Biol. 2013 Dec 4.

    Insert

    ERGIC2 (ERGIC2 Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    4 base deletion at codon #189

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

19. OMM PKA Reporter

    Plasmid

    #104550

    Purpose

    Expresses Tom20-mTurquoise2-VASP at the outer mitochondrial membrane of mammalian cells.

    Depositor

    David Lawrence

    Article

    O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

    Insert

    Tom20 - mTurquoise2 - VASP 148-164
    

    Tags

    mTurqouise2

    Expression

    Mammalian

    Promoter

    EF1 alpha

    Available Since

    March 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. LR (bact: HyPer)

    Plasmid

    #162069

    Purpose

    Used for the diffuse visualization of H2O2 production in cells.

    Depositor

    Philipp Niethammer

    Article

    Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

    Insert

    beta actin 2 (actb2 Zebrafish)
    

    Use

    Gateway donor vector

    Tags

    HyPer

    Promoter

    T3

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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