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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,712 results for: NSI

Showing: 9481 - 9500 of 11712 results

  1. pCWU50

    Plasmid
    #232318
    Purpose
    A conditional plasmid system where RepA, required for pCWU6-based plasmid replication, is regulated by an inducible fdx promoter and theophylline-responsive riboswitch
    Depositor
    Chenggang Wu
    Insert
    Pfdx-E
    Expression
    Bacterial
    Promoter
    fdx
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    232318-plasmid-map-sequence-id-462281

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  2. pOpen-phi29pol

    Plasmid
    #165573
    Purpose
    DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    phi29 DNA Polymerase
    Use
    Synthetic Biology
    Available Since
    Aug. 24, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165573-plasmid-map-sequence-id-340935

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  3. pGR595

    Plasmid
    #213785
    Purpose
    Integrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.
    Depositor
    Chang Liu
    Insert
    BadBoy2 TP-DNAP1
    Use
    Synthetic Biology
    Expression
    Yeast
    Available Since
    March 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    213785-plasmid-map-sequence-id-424581

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  4. MUT_S1-H1

    Plasmid
    #218394
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218394-plasmid-map-sequence-id-433909

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  5. pENN.AAV.CamKII.GCaMP6f.WPRE.SV40

    Plasmid
    #100834
    Purpose
    AAV expression of ultrasensitive protein calcium sensor from CamKII promoter
    Depositor
    James M. Wilson
    Has Service
    AAV1, AAV5, and AAV9
    Insert
    GCaMP6f
    Use
    AAV
    Expression
    Mammalian
    Mutation
    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G
    Promoter
    CamKII
    Available Since
    March 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    100834-plasmid-map-sequence-id-259370

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  6. pSL1180-HR-PUbECFP

    Plasmid
    #47917
    Purpose
    Donor plasmid for homologous recombination that expresses ECFP consitutively using the Aedes aegypti polyubiquitin (PUb) promoter.
    Depositor
    Leslie Vosshall
    Insert
    ECFP
    Tags
    NLS
    Expression
    Insect
    Promoter
    Aedes aegypti polyubiquitin (PUb)
    Available Since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only
    47917-plasmid-map-sequence-id-68604

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  7. p-dCas9-VP64-Hygro

    Plasmid
    #104405
    Purpose
    transient expression of dCas9-VP64 fusion protein
    Depositor
    Stephan Beck
    Insert
    VP64
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104405-plasmid-map-sequence-id-204462

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  8. FFTTH--GFP-Amotl2

    Plasmid
    #112833
    Purpose
    transient/stable overexpression
    Depositor
    Tomasz Prószyński
    Insert
    Amotl2 (Amotl2 Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Available Since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112833-plasmid-map-sequence-id-217927

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  9. TAP-GFP-Amotl2

    Plasmid
    #112835
    Purpose
    transient/stable overexpression
    Depositor
    Tomasz Prószyński
    Insert
    Amotl2 (Amotl2 Mouse)
    Tags
    GFP and TAP
    Expression
    Mammalian
    Available Since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112835-plasmid-map-sequence-id-217938

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  10. CL20_mEGFP_FOidr_131

    Plasmid
    #244683
    Purpose
    Express mEGFP-tagged intrinsically disordered protein (IDR), FOidr_131, derived from human fusion protein PAX5_BZW1; PAX5_CBFA2T3; PAX5_ESRRA; PAX5_FOXP1; PAX5_JAK2; PAX5_KANK1; PAX5_NCOA5; PAX5_NOL4L; PAX5_PAX5; PAX5_ZCCHC7; PAX5_ZNF276; PAX5_ZNF521; ZCCHC7_PAX5
    Depositor
    Richard Kriwacki
    Insert
    FOidr_131
    Tags
    monomeric EGFP
    Expression
    Mammalian
    Mutation
    Unmutated
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    244683-plasmid-map-sequence-id-488154

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  11. MUT_S7-H1

    Plasmid
    #218396
    Purpose
    3-exon minigene, with SRSF7 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218396-plasmid-map-sequence-id-433914

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  12. TAP-Amotl2

    Plasmid
    #112834
    Purpose
    transient/stable overexpression
    Depositor
    Tomasz Prószyński
    Insert
    Amotl2 (Amotl2 Mouse)
    Tags
    TAP
    Expression
    Mammalian
    Available Since
    Aug. 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    112834-plasmid-map-sequence-id-217933

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  15. pAM-35s-CBL4-YFPc-1

    Plasmid
    #102396
    Purpose
    split YFP. Plant expression of CBL4-YFPc-1
    Depositor
    Silke Robatzek
    Insert
    CBL4 (SOS3 Mustard Weed)
    Tags
    YFPc
    Expression
    Plant
    Promoter
    35S
    Available Since
    Nov. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    102396-plasmid-map-sequence-id-197841

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  16. p2B-58

    Plasmid
    #177928
    Purpose
    Expresses yeGFP under control of doxycycline-responsive TET4 promoter (contains four rtTA binding sites)
    Depositor
    Vadim Gladyshev
    Article
    Barre et al (unpublished)
    Insert
    EGFP
    Expression
    Bacterial and Yeast
    Promoter
    TET4 (contains four rtTA binding sites)
    Available Since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177928-plasmid-map-sequence-id-349996

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  17. pGR596

    Plasmid
    #213787
    Purpose
    Integrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette
    Depositor
    Chang Liu
    Insert
    BadBoy2 TP-DNAP1
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Mutation
    See manuscript
    Available Since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213787-plasmid-map-sequence-id-424625

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  20. pGR618

    Plasmid
    #213786
    Purpose
    Integrates at the CAN1 locus the BB-Tv TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette
    Depositor
    Chang Liu
    Insert
    BB-Tv TP-DNAP1
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213786-plasmid-map-sequence-id-424624

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Showing: 9481 - 9500 of 11712 results