We narrowed to 12,028 results for: LEA
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Plasmid#222429PurposeFRET-based reporter for monitoring deltaPKC activity at the plasma membrane in cells.DepositorInsertPlasma membrane delta-selective C kinase activity reporter
TagsCFP and YFPExpressionMammalianAvailable SinceJan. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
Cdk13_intron4_sg3_pX330
Plasmid#127340PurposesgRNA that cuts within intron 4 of mouse CDK13 genomic locus- guide #3DepositorInsertMouse Cdk13 Intron 4 Targeting sgRNA (Cdk13 Mouse)
UseCRISPR and Mouse TargetingPromoterU6 PromoterAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMDC Renilla Luc SRE
Plasmid#191657PurposeExpressed Renilla Luciferase with SOX resistance elementDepositorInsertRenilla Luciferase SRE
ExpressionMammalianPromoterCMVAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac SOX Q129H
Plasmid#192057PurposeExpression vector for SOX (ORF37) Q129HDepositorInsertSOX (ORF37) Q129H
ExpressionInsectMutationQ129HPromoterPolyhedrinAvailable SinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-PKP3(305-797)
Plasmid#202599PurposeExpresses ARM domain of plakophilin3DepositorInsertplakophilin3 (PKP3 Human)
TagsHis8 tag with precision protease cleavage siteExpressionBacterialMutationDeleted amino acids 1 to 304Available SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NRCH-CGBE1
Plasmid#198557PurposeExpresses human codon-optimized SpCas9-NRCH-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NRCH-CGBE1-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRCH-CGBE1
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
CPH3949_HIS-MBP-TEV-Tom1p-HECT-2850-3268
Plasmid#227089PurposeBacterial expression of HIS-MBP-TEV tagged Tom1p HECT domain (2850-3268)DepositorInserttom1p-HECT (TOM1 Budding Yeast)
TagsHIS-MBP-TEV_cleavage_siteExpressionBacterialPromoterT7Available SinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
CPH3747_GST-TEV-Spt6p-tSH2-1223-1452
Plasmid#227086PurposeBacterial expression of R1282H single-mutant GST-tagged Spt6 tSH2 domain (1223-1452)DepositorInsertspt6-tsh2 (SPT6 Budding Yeast)
TagsGST-TEV_cleavage_siteExpressionBacterialMutationR1282HPromoterT7Available SinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKM-U6-reRNA-mmSCN9A
Plasmid#205636PurposereRNA guide targeting Mus Musculus SCN9ADepositorAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET41-FeoB-OL2del
Plasmid#216742PurposeStudy the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 2 (OL2) by examining the variant lacking OL2 in its interaction with exogenous peptides.DepositorInsertfeoB (PA4358 P. aeruginosa (Bacteria))
Tags8x HisExpressionBacterialMutationOuter loop 2 (OL2) with the sequence of [LEDSGYMA…Available SinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
mTph2p(330bp)-Luc
Plasmid#223663PurposeFirefly-luciferase reporter expression driven by proximal 330-bp of mouse Tph2 promoterDepositorInsertmTph2 promoter (Tph2 Mouse)
UseLuciferaseTagsFirefly luciferaseExpressionMammalianPromotermTph2 promoterAvailable SinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-ScChd1[R126A/R130A]
Plasmid#221197PurposeScChd1-aa118-1274 chromatin remodeler with R126A and R130A mutationsDepositorInsertScChd1 (aa118-1274)
Tags6xHis-tag (Precission protease cleavable)ExpressionBacterialMutationR126A, R130APromoterT7Available SinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-ScChd1[∆ChEx]
Plasmid#221199PurposeScChd1-aa142-1274 chromatin remodeler with ChEx domain deletedDepositorInsertScChd1 (aa142-1274)
Tags6xHis-tag (Precission protease cleavable)ExpressionBacterialMutationdeleted amino acids 1-117 and 1275-1468Available SinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAVED-CHAT-cbm_PCaspase
Plasmid#214047PurposeBacterial expression plasmid for SAVED-CHAT cA3 binding pocket mutant and PCaspase from Haliangium ochraceumDepositorInsertSAVED-CHAT-PCaspase
ExpressionBacterialMutationK121E, Q122A, N274A, R276E, Y285A, F286APromoterlacUV5 promoterAvailable SinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-ArgiNLS-mVenus-Q69M(ME)
Plasmid#220597PurposeConstitutive expression of a single-cell discriminating version of mVenus-Q69M (ME) fluorescent protein.DepositorInsertmVenus-Q69M (ME)
UseAAVTagsArgiNLSExpressionMammalianPromoterEF1aAvailable SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tol2-U6.3-sgRNA-DNM1L -GFP
Plasmid#221845PurposeTol2 transposon expressing sgRNA targeting chick DNM1L from chick U6.3 promoter expresses GFP reporter from GAGC promoterDepositorInsertsEGFP
DNM1L sgRNA-gTGTTTTCCGACCATCCTCTG
UseCRISPR; Tol2 transposon optimised for chick expre…ExpressionMammalianPromoterCAGC and U6.3 chickAvailable SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tol2-U6.3-sgRNA-MFN1-GFP
Plasmid#221846PurposeTol2 transposon expressing sgRNA targeting chick MFN1 from chick U6.3 promoter expresses GFP reporter from GAGC promoteDepositorInsertsEGFP
MFN1 sgRNA-GAGAAGAAGAGCGTCAAGGT
UseCRISPR; Tol2 transposon optimised for chick expre…ExpressionMammalianPromoterCAGC and U6.3 chickAvailable SinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHis-WzzE
Plasmid#196661PurposeExpression of N-terminally His6-tagged WzzE from Pectobacterium atrosepticum in E.coliDepositorInsertWzzE
TagsTEV-cleavable His6ExpressionBacterialPromoterT7Available SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGST-WzzE
Plasmid#196662PurposeExpression of N-terminally GST-tagged WzzE from Pectobacterium atrosepticum in E.coliDepositorInsertWzzE
TagsTEV-cleavable GSTExpressionBacterialMutationValine insertion at position 2.PromoterT7Available SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWzyE-GST-distrepII
Plasmid#196666PurposeExpression of C-terminally GST-distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
TagsGST and TEV-cleavable di-strep tag IIExpressionBacterialPromoterT7Available SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only