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pMOD_A0808

Plasmid

#91024

Purpose

Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_dead (D10A + H840A), Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9_dead (D10A + H840A)

Use

CRISPR

Mutation

D10A, H840A

Promoter

EC1.2

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A0408

Plasmid

#91010

Purpose

Module A, Promoter: EC1.2, Gene: AtCas9_dead (D10A + H840A), Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_dead (D10A + H840A)

Use

CRISPR

Mutation

D10A, H840A

Promoter

EC1.2

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_A0708

Plasmid

#91021

Purpose

Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_H840A nickase, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-AtCas9_H840A nickase

Use

CRISPR

Mutation

H840A

Promoter

EC1.2

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1d(C94S)-Myc

Plasmid

#91935

Purpose

Bacterial expression of maize SUMO1a and inactive SCE1d

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1d(C94S)-Myc

Tags

6His and Myc

Expression

Bacterial

Mutation

C94S active site mutation and Truncation of ZmSUM…

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1b(C94S)-Myc

Plasmid

#91933

Purpose

Bacterial expression of maize SUMO1a and inactive SCE1b

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1b(C94S)-Myc

Tags

6His and Myc

Expression

Bacterial

Mutation

C94S active site mutation and Truncation of ZmSUM…

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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px330-MRPsg3

Plasmid

#87191

Purpose

disruption of human RMRP gene via CRISPR-Cas9, guide 3

Depositor

Thomas Cech

Article

Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

Insert

human MRP RNA CRISPR guide #3 (RMRP Human)

Use

CRISPR

Expression

Mammalian

Promoter

human U6

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBRC169-DXMT

Plasmid

#79822

Purpose

DsREDmonomer-based BiFC vector for plants (Positive control: DXMT)

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Insert

CaDXMT1

Use

Reporter plasmid

Tags

RC169/DsREDmonomer (169–225)

Expression

Plant

Promoter

CaMV 35S promoter and CaMV35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pBRN168-DXMT

Plasmid

#79821

Purpose

DsREDmonomer-based BiFC vector for plants (Positive control: DXMT)

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Insert

CaDXMT1

Use

Reporter plasmid

Tags

RN168/DsREDmonomer (1–168)

Expression

Plant

Promoter

CaMV 35S promoter and CaMV35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pBRC169-MXMT

Plasmid

#79820

Purpose

DsREDmonomer-based BiFC vector for plants (Positive control: MXMT)

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Insert

CaMXMT1

Use

Reporter plasmid

Tags

RC169/DsREDmonomer (169–225)

Expression

Plant

Promoter

CaMV 35S promoter and CaMV35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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csd5_41-192/pET28b(+)

Plasmid

#83296

Purpose

csd5, construct: 41-192, pET28b(+) N-tag

Depositor

SeWon Suh

Article

Kim et al Acta Crystallogr D Biol Crystallogr. 201

Insert

hp1250

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-40

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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csd4_22-438/pET28b(+)

Plasmid

#83295

Purpose

csd4, construct: 22-438, pET28b(+) N-tag

Depositor

SeWon Suh

Article

Kim et al Acta Crystallogr D Biol Crystallogr. 201

Insert

hp1075

Tags

His tag

Expression

Bacterial

Mutation

deleted amino acid 1-21

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only
pmcsg7-mCRT(D311A)

Plasmid

#83509

Purpose

Bacterial expression of Calreticulin mutant

Depositor

Malini Raghavan

Article

Wijeyesakere et al J Biol Chem. 2011 Mar 18;286(11):8771-85

Insert

CALR (Calr Mouse)

Tags

His

Expression

Bacterial

Mutation

D311A

Promoter

T7

Available Since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only
pmcsg7-mCRT(1-351)

Plasmid

#83508

Purpose

Bacterial expression of Calreticulin mutant

Depositor

Malini Raghavan

Article

Wijeyesakere et al J Biol Chem. 2011 Mar 18;286(11):8771-85

Insert

CALR (Calr Mouse)

Tags

His

Expression

Bacterial

Mutation

1-351 only

Promoter

T7

Available Since

Oct. 12, 2016

Availability

Academic Institutions and Nonprofits only
pDONR-IAA8_no stop

Plasmid

#83874

Purpose

Gateway donor vector for IAA8 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

indole-3-acetic acid inducible 8 (IAA8 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA5_no stop

Plasmid

#83868

Purpose

Gateway donor vector for IAA5 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA5 (IAA5 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA3_no stop

Plasmid

#83863

Purpose

Gateway donor vector for IAA3 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA3 (SHY2 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA10_no stop

Plasmid

#83848

Purpose

Gateway donor vector for IAA10 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

indoleacetic acid-induced protein 10 (IAA10 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA4_no stop

Plasmid

#83866

Purpose

Gateway donor vector for IAA4 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA4 (ATAUX2-11 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA28_no stop

Plasmid

#83861

Purpose

Gateway donor vector for IAA28 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

NP_568478.1 (IAA28 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA15_no stop

Plasmid

#83853

Purpose

Gateway donor vector for IAA15 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

indole-3-acetic acid inducible 15 (IAA15 Mustard Weed)

Use

Gateway donor vector

Mutation

no stop codon

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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